Construct: ORF TRCN0000479746
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004511.1_s317c1
- Derived from:
- ccsbBroadEn_11249
- DNA Barcode:
- TGGCTCGCTACCTCCTCGCAGCAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ST7 (7982)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479746
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7982 | ST7 | suppression of tumorigenici... | NM_018412.4 | 98.7% | 98.7% | 344_364del |
| 2 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369598.1 | 94.8% | 94.8% | 344_364del;641_709del |
| 3 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369604.1 | 94.3% | 92.5% | (many diffs) |
| 4 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369601.1 | 93.9% | 93.7% | 344_364del;641_709del;1390_1391insGTACGTGGGAAGGCA |
| 5 | human | 7982 | ST7 | suppression of tumorigenici... | NM_021908.3 | 90.6% | 88.9% | (many diffs) |
| 6 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369600.1 | 90.5% | 89.7% | (many diffs) |
| 7 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369603.1 | 90.1% | 89.8% | (many diffs) |
| 8 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369607.1 | 89.6% | 89.5% | 1_1delAins151;194_214del |
| 9 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369599.1 | 86.9% | 86.1% | (many diffs) |
| 10 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369602.1 | 86.5% | 86.3% | (many diffs) |
| 11 | human | 7982 | ST7 | suppression of tumorigenici... | NM_001369606.1 | 86% | 85.9% | 1_1delAins151;194_214del;491_559del |
| 12 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161423.1 | 76.9% | (many diffs) | |
| 13 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161421.1 | 75.9% | (many diffs) | |
| 14 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161422.1 | 74.4% | (many diffs) | |
| 15 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161420.1 | 73.6% | (many diffs) | |
| 16 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161419.1 | 67.2% | (many diffs) | |
| 17 | human | 7982 | ST7 | suppression of tumorigenici... | NR_161418.1 | 65.3% | (many diffs) | |
| 18 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001289626.1 | 90.6% | 98% | (many diffs) |
| 19 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001289624.1 | 87% | 94.1% | (many diffs) |
| 20 | mouse | 64213 | St7 | suppression of tumorigenici... | XM_011241074.1 | 87% | 94.1% | (many diffs) |
| 21 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001289625.1 | 86.1% | 93% | (many diffs) |
| 22 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001083315.2 | 83.4% | 89.7% | (many diffs) |
| 23 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001289629.1 | 83.1% | 89.7% | (many diffs) |
| 24 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_022332.3 | 80.1% | 86.1% | (many diffs) |
| 25 | mouse | 64213 | St7 | suppression of tumorigenici... | NM_001289627.1 | 79.8% | 86.1% | (many diffs) |
| 26 | mouse | 64213 | St7 | suppression of tumorigenici... | XM_011241076.2 | 62.9% | 68.4% | (many diffs) |
| 27 | mouse | 64213 | St7 | suppression of tumorigenici... | XM_011241075.2 | 55.9% | 61.6% | (many diffs) |
| 28 | mouse | 64213 | St7 | suppression of tumorigenici... | XR_377291.3 | 50.9% | (many diffs) | |
| 29 | mouse | 64213 | St7 | suppression of tumorigenici... | XR_868763.2 | 50.5% | (many diffs) | |
| 30 | mouse | 64213 | St7 | suppression of tumorigenici... | XR_377290.3 | 49.5% | (many diffs) | |
| 31 | mouse | 64213 | St7 | suppression of tumorigenici... | XR_868764.2 | 33.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1707
- ORF length:
- 1641
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgaagcggcc acgggctttc tggagcagct caagtcctgc atagtttggt 121 cttggacgta tctgtggacc gtgtggttct tcatcgtgct attcctggtc tacatcctgc 181 gggtgccttt gaaaatcaac gacaacttga gcacagtgag catgtttttg aacacattaa 241 caccgaagtt ctacgtggcc ctaacaggca cttcctcact aatatcaggg cttattttga 301 tatttgaatg gtggtatttt cgcaaatacg gaacttcatt cattgaacaa gtctcagtaa 361 gccacttgcg cccccttctg ggaggggttg acaacaactc ttccaacaac tcagattcca 421 ataggcaaag tgtctcagaa tgcaaagtat ggcgaaatcc actaaattta tttaggggtg 481 ctgaatacaa tcggtatact tgggtgacag gacgagagcc tcttacttac tatgacatga 541 atctctctgc ccaagaccac cagacattct ttacttgtga ctcggaccat ctgcgtcccg 601 cagatgcaat aatgcagaaa gcctggagag agagaaaccc ccaagctagg atttctgcag 661 ctcatgaagc cttggagata aatgagtgtg caactgctta tattctcttg gctgaagagg 721 aagcaacaac cattgctgaa gcagaaaaat tatttaagca ggccctgaag gctggagatg 781 gctgttaccg acgctctcag cagctacaac atcatggatc ccagtatgaa gcccaacata 841 gacgagacac caatgtcttg gtgtacatca aaagaaggct agcaatgtgt gccagaagac 901 tcgggaggac cagggaagca gtgaaaatga tgagagattt aatgaaggag ttcccccttc 961 tgagtatgtt caatatccat gaaaaccttt tagaagccct tctggaacta caagcatatg 1021 ctgatgttca ggcagtctta gcaaagtatg atgatataag cttaccaaag tcagcaacaa 1081 tatgctacac agctgctttg ctcaaagcaa gagctgtctc tgacaaattc tctcctgagg 1141 ctgcatctcg gcgggggctg agcacagcag agatgaatgc agtagaggcc attcatagag 1201 ctgtggaatt caatcctcat gtgccaaaat acctactaga aatgaaaagc ttaatcctac 1261 ccccagaaca tatcctgaag agaggagaca gtgaagcaat agcatatgca ttctttcatc 1321 ttgcacactg gaagagagtg gaaggggctt tgaaTCTTTT GCATTGTACG TGGGAAGGCA 1381 CTTTTCGGAT GATCCCTTAT CCCTTGGAAA AGGGGCACCT ATTTTATCCT TACCCAATCT 1441 GTACAGAAAC AGCAGACCGA GAGCTGCTTC CATCTTTCCA TGAAGTCTCA GTTTACCCAA 1501 AGAAGGAGCT TCCCTTCTTT ATTCTCTTTA CTGCTGGATT ATGTTCCTTC ACAGCCATGC 1561 TGGCCCTCCT GACACATCAG TTCCCGGAAC TTATGGGGGT CTTCGCAAAA GCTTTCCTCA 1621 GCACTTTGTT TGCCCCCTTA AACTTTGTCA TGGAGAAAGT GGAGAGCATC CTCCCATCCA 1681 GTCTGTGGCA CCAGCTAACA CGGATCTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1741 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1801 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GATGGCTCGC 1861 TACCTCCTCG CAGCACACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1921 aagatt