Transcript: Mouse XM_006537659.2

PREDICTED: Mus musculus muscle, skeletal, receptor tyrosine kinase (Musk), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Musk (18198)
Length:
7189
CDS:
388..3054

Additional Resources:

NCBI RefSeq record:
XM_006537659.2
NBCI Gene record:
Musk (18198)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006537659.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000361387 TGCACAAGACTGCCATATTTA pLKO_005 1786 CDS 100% 15.000 21.000 N Musk n/a
2 TRCN0000361321 ATAACAACATTCCCGTCAATA pLKO_005 1825 CDS 100% 13.200 18.480 N Musk n/a
3 TRCN0000023688 CCGAGGAATAACATTGAGTAT pLKO.1 2155 CDS 100% 4.950 6.930 N Musk n/a
4 TRCN0000023685 GCCCGAGTCTATCTTCTACAA pLKO.1 2754 CDS 100% 4.950 6.930 N Musk n/a
5 TRCN0000023684 GCTACCAATAAGCACGGAGAA pLKO.1 1297 CDS 100% 4.050 3.240 N Musk n/a
6 TRCN0000356283 ACATGCATAGCTACCAATAAG pLKO_005 1288 CDS 100% 13.200 9.240 N MUSK n/a
7 TRCN0000361449 ACATGCATAGCTACCAATAAG pLKO_005 1288 CDS 100% 13.200 9.240 N Musk n/a
8 TRCN0000023686 CCTGCACAAGACTGCCATATT pLKO.1 1784 CDS 100% 13.200 9.240 N Musk n/a
9 TRCN0000195290 CAAGTGAAGATGAAACCTAAA pLKO.1 733 CDS 100% 10.800 7.560 N MUSK n/a
10 TRCN0000002165 CCAGGACTCTACACATGCATA pLKO.1 1276 CDS 100% 4.950 3.465 N MUSK n/a
11 TRCN0000023687 CCCAACATTGTGAAACTCTTA pLKO.1 2350 CDS 100% 4.950 3.465 N Musk n/a
12 TRCN0000166364 CACACACACACACACACACAA pLKO.1 5307 3UTR 100% 4.950 2.475 Y KAAG1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006537659.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14704 pDONR223 0% 85.9% 91% None (many diffs) n/a
2 ccsbBroad304_14704 pLX_304 0% 85.9% 91% V5 (many diffs) n/a
3 ccsbBroadEn_06606 pDONR223 100% 77.5% 82.5% None (many diffs) n/a
4 ccsbBroad304_06606 pLX_304 0% 77.5% 82.5% V5 (many diffs) n/a
5 TRCN0000465498 ACCCGCCCGAGAACTTTAAAAATA pLX_317 15.3% 77.5% 82.5% V5 (many diffs) n/a
6 TRCN0000488630 CAGTCTGCATCGTGCCGCATACGT pLX_317 24% 31.5% 34.5% V5 (not translated due to prior stop codon) (many diffs) n/a
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