Construct: ORF TRCN0000466541

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006136.1_s317c1
Derived from:
ccsbBroadEn_07408
DNA Barcode:
ACCCTTTCAGGCTTCGACCGAGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TGFBRAP1 (9392)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000466541
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9392 TGFBRAP1 transforming growth factor ... NM_001142621.3 99.8% 100% (many diffs)
2 human 9392 TGFBRAP1 transforming growth factor ... NM_004257.6 99.8% 100% (many diffs)
3 human 9392 TGFBRAP1 transforming growth factor ... NM_001328646.2 94.7% 92.8% (many diffs)
4 human 9392 TGFBRAP1 transforming growth factor ... XM_017005339.2 51.1% 51.1% 0_1ins1260
5 human 9392 TGFBRAP1 transforming growth factor ... NR_137328.1 46% (many diffs)
6 mouse 73122 Tgfbrap1 transforming growth factor,... NM_001013025.2 86% 89.3% (many diffs)
7 mouse 73122 Tgfbrap1 transforming growth factor,... XM_006496301.3 86% 89.3% (many diffs)
8 mouse 73122 Tgfbrap1 transforming growth factor,... XM_006496302.3 86% 89.3% (many diffs)
9 mouse 73122 Tgfbrap1 transforming growth factor,... XM_006496303.2 86% 89.3% (many diffs)
10 mouse 73122 Tgfbrap1 transforming growth factor,... XM_011238603.2 85.2% 88.4% (many diffs)
11 mouse 73122 Tgfbrap1 transforming growth factor,... XM_011238604.2 85.2% 88.4% (many diffs)
12 mouse 73122 Tgfbrap1 transforming growth factor,... XM_006496300.2 85.1% 88.3% (many diffs)
13 mouse 73122 Tgfbrap1 transforming growth factor,... XM_017312455.1 57.7% 59.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2646
ORF length:
2580
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat gagcatcaaa gcctttacgc ttgtctctgc tgtggagcgg gagctgctga
121tgggcgacaa ggagcgcgtc aacatagagt gcgtggagtg ctgcggcagg gacctctacg
181tgggcaccaa cgactgcttc gtctaccact tcctgttgga ggagaggcca gtgcctgctg
241ggccagccac gttcactgcc accaaacagc tgcagagaca cttgggcttc aagaagcccg
301tgaacgagct gcgcgcggcc tcagcactca acaggctgct ggtgctgtgt gacaactcca
361tcagcctggt caacatgctg aacctcgagc cagtgccttc gggggcccgc atcaaggggg
421cagccacgtt tgcactgaac gagaaccctg tgagtgggga ccccttctgt gtagaagttt
481gcatcatctc tgtcaaacgc agaaccatcc agatgtttct ggtgtacgag gaccgggtgc
541agatcgtcaa ggaggtgtcg actgccgaac agcccctcgc tgtggctgtg gacggccact
601tcctgtgtct ggctctgacc actcagtaca tcatccacaa ttacagcaca ggcgtctccc
661aggacctttt tccctactgc agtgaggaga ggccgccgat cgtcaagagg atagggagac
721aggagttcct gctggcgggc cccggagggc tgggcatgtt tgccacagtc gcagggatat
781cccagcgtgc ccccgtgcac tggtcggaga atgtgattgg ggcggctgtg tcctttccat
841acgtcatagc gctcgatgac gaattcatca cagtccacag catgttggat cagcaacaga
901agcagacgct gccctttaag gagggccata tcctacagga ctttgaagga agagtgatcg
961ttgccacaag taaaggagtt tacatcttgg ttccattacc tttggaaaaa caaatacagg
1021atcttctagc aagccgcaga gtagaagagg ctttggtttt agcaaaagga gcccggagga
1081acattccaaa ggaaaaattt caggtaatgt acagaaggat tctgcagcag gcgggattta
1141tacagtttgc acaacttcag ttcctggaag ctaaagagct cttcagaagc ggccagcttg
1201atgtccggga gctgatctct ctctacccct tcctgttgcc cacctcctcc tccttcaccc
1261ggtcccaccc tcctcttcat gagtacgcag acctgaacca gctgacccag ggggaccagg
1321agaagatggc caagtgcaaa cgcttcctca tgagctacct gaacgaggtc cgcagcacag
1381aggtagcaaa tggctacaag gaggacatcg acacagcctt gctcaaactg tatgcagagg
1441ctgaccacga cagcctgctg gacctcctgg tcactgagaa cttctgtctt ctgacggaca
1501gtgctgcctg gctagagaag cacaaaaagt attttgcact tggactgctc tatcattata
1561ataaccaaga tgctgctgca gttcagttgt gggtgaacat tgtgaatggc gatgtccagg
1621actccacacg ctcagacctg tatgaataca tcgtggattt tcttacctac tgcttagacg
1681aggaactagt gtgggcctat gctgattggg tcctgcagaa aagtgaagag gtcggagttc
1741aggttttcac caagagacct ttggatgaac agcagaagaa cagttttaat ccagacgaca
1801ttatcaattg ccttaaaaaa taccctaaag cccttgtgaa gtatctggaa catcttgtga
1861tagacaagag actgcagaaa gaagagtatc acacccactt agctgtgctg tacctggaag
1921aggtgctgct gcagagggcc tccgccagtg gcaagggtgc agaggccacc gagacgcagg
1981ccaagctgcg gcggctgctc cagaaatctg atttataccg agtccacttt cttctcgaga
2041ggctgcaggg agctggcctg cccatggaga gcgccatcct gcacgggaag ctgggcgagc
2101atgagaaggc gctgcatatc ctggtgcacg agctgcagga ctttgcagcg gccgaggact
2161actgcctgtg gtgctccgag ggccgagacc caccccaccg ccagcaactc tttcacacgc
2221tgctggccat ctacctgcat gctggcccca ctgcccacga gctggccgtg gctgccgtgg
2281acctgctgaa ccgccacgcc accgaatttg atgcagccca ggtgctgcag atgctgccTG
2341ACACCTGGTC AGTGCAGCTC CTCTGCCCAT TCCTGATGGG GGCCATGAGG GACAGCATCC
2401ATGCCAGGAG GACCATGCAG GTGGCTCTCG GCCTGGCCAG GTCCGAAAAC TTAATCTACA
2461CCTACGATAA GATGAAGTTG AAAGGAAGCT CAATCCAACT CTCAGACAAA AAGCTTTGTC
2521AGATATGCCA AAATCCCTTT TGTGAGCCTG TGTTTGTTAG ATACCCAAAT GGTGGTCTTG
2581TGCACACCCA CTGTGCCGCC AGCAGACACA CAAACCCCAG CTCATCCAGT CCTGGCACTC
2641GGACTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
2701TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
2761CTTGGCTTTA TATATCTTGT GGAAAGGACG AACCCTTTCA GGCTTCGACC GAGTTACGCG
2821TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)