Construct: ORF TRCN0000466541
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006136.1_s317c1
- Derived from:
- ccsbBroadEn_07408
- DNA Barcode:
- ACCCTTTCAGGCTTCGACCGAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TGFBRAP1 (9392)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000466541
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9392 | TGFBRAP1 | transforming growth factor ... | NM_001142621.3 | 99.8% | 100% | (many diffs) |
| 2 | human | 9392 | TGFBRAP1 | transforming growth factor ... | NM_004257.6 | 99.8% | 100% | (many diffs) |
| 3 | human | 9392 | TGFBRAP1 | transforming growth factor ... | NM_001328646.2 | 94.7% | 92.8% | (many diffs) |
| 4 | human | 9392 | TGFBRAP1 | transforming growth factor ... | XM_017005339.2 | 51.1% | 51.1% | 0_1ins1260 |
| 5 | human | 9392 | TGFBRAP1 | transforming growth factor ... | NR_137328.1 | 46% | (many diffs) | |
| 6 | mouse | 73122 | Tgfbrap1 | transforming growth factor,... | NM_001013025.2 | 86% | 89.3% | (many diffs) |
| 7 | mouse | 73122 | Tgfbrap1 | transforming growth factor,... | XM_006496301.3 | 86% | 89.3% | (many diffs) |
| 8 | mouse | 73122 | Tgfbrap1 | transforming growth factor,... | XM_006496302.3 | 86% | 89.3% | (many diffs) |
| 9 | mouse | 73122 | Tgfbrap1 | transforming growth factor,... | XM_006496303.2 | 86% | 89.3% | (many diffs) |
| 10 | mouse | 73122 | Tgfbrap1 | transforming growth factor,... | XM_011238603.2 | 85.2% | 88.4% | (many diffs) |
| 11 | mouse | 73122 | Tgfbrap1 | transforming growth factor,... | XM_011238604.2 | 85.2% | 88.4% | (many diffs) |
| 12 | mouse | 73122 | Tgfbrap1 | transforming growth factor,... | XM_006496300.2 | 85.1% | 88.3% | (many diffs) |
| 13 | mouse | 73122 | Tgfbrap1 | transforming growth factor,... | XM_017312455.1 | 57.7% | 59.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2646
- ORF length:
- 2580
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat gagcatcaaa gcctttacgc ttgtctctgc tgtggagcgg gagctgctga 121 tgggcgacaa ggagcgcgtc aacatagagt gcgtggagtg ctgcggcagg gacctctacg 181 tgggcaccaa cgactgcttc gtctaccact tcctgttgga ggagaggcca gtgcctgctg 241 ggccagccac gttcactgcc accaaacagc tgcagagaca cttgggcttc aagaagcccg 301 tgaacgagct gcgcgcggcc tcagcactca acaggctgct ggtgctgtgt gacaactcca 361 tcagcctggt caacatgctg aacctcgagc cagtgccttc gggggcccgc atcaaggggg 421 cagccacgtt tgcactgaac gagaaccctg tgagtgggga ccccttctgt gtagaagttt 481 gcatcatctc tgtcaaacgc agaaccatcc agatgtttct ggtgtacgag gaccgggtgc 541 agatcgtcaa ggaggtgtcg actgccgaac agcccctcgc tgtggctgtg gacggccact 601 tcctgtgtct ggctctgacc actcagtaca tcatccacaa ttacagcaca ggcgtctccc 661 aggacctttt tccctactgc agtgaggaga ggccgccgat cgtcaagagg atagggagac 721 aggagttcct gctggcgggc cccggagggc tgggcatgtt tgccacagtc gcagggatat 781 cccagcgtgc ccccgtgcac tggtcggaga atgtgattgg ggcggctgtg tcctttccat 841 acgtcatagc gctcgatgac gaattcatca cagtccacag catgttggat cagcaacaga 901 agcagacgct gccctttaag gagggccata tcctacagga ctttgaagga agagtgatcg 961 ttgccacaag taaaggagtt tacatcttgg ttccattacc tttggaaaaa caaatacagg 1021 atcttctagc aagccgcaga gtagaagagg ctttggtttt agcaaaagga gcccggagga 1081 acattccaaa ggaaaaattt caggtaatgt acagaaggat tctgcagcag gcgggattta 1141 tacagtttgc acaacttcag ttcctggaag ctaaagagct cttcagaagc ggccagcttg 1201 atgtccggga gctgatctct ctctacccct tcctgttgcc cacctcctcc tccttcaccc 1261 ggtcccaccc tcctcttcat gagtacgcag acctgaacca gctgacccag ggggaccagg 1321 agaagatggc caagtgcaaa cgcttcctca tgagctacct gaacgaggtc cgcagcacag 1381 aggtagcaaa tggctacaag gaggacatcg acacagcctt gctcaaactg tatgcagagg 1441 ctgaccacga cagcctgctg gacctcctgg tcactgagaa cttctgtctt ctgacggaca 1501 gtgctgcctg gctagagaag cacaaaaagt attttgcact tggactgctc tatcattata 1561 ataaccaaga tgctgctgca gttcagttgt gggtgaacat tgtgaatggc gatgtccagg 1621 actccacacg ctcagacctg tatgaataca tcgtggattt tcttacctac tgcttagacg 1681 aggaactagt gtgggcctat gctgattggg tcctgcagaa aagtgaagag gtcggagttc 1741 aggttttcac caagagacct ttggatgaac agcagaagaa cagttttaat ccagacgaca 1801 ttatcaattg ccttaaaaaa taccctaaag cccttgtgaa gtatctggaa catcttgtga 1861 tagacaagag actgcagaaa gaagagtatc acacccactt agctgtgctg tacctggaag 1921 aggtgctgct gcagagggcc tccgccagtg gcaagggtgc agaggccacc gagacgcagg 1981 ccaagctgcg gcggctgctc cagaaatctg atttataccg agtccacttt cttctcgaga 2041 ggctgcaggg agctggcctg cccatggaga gcgccatcct gcacgggaag ctgggcgagc 2101 atgagaaggc gctgcatatc ctggtgcacg agctgcagga ctttgcagcg gccgaggact 2161 actgcctgtg gtgctccgag ggccgagacc caccccaccg ccagcaactc tttcacacgc 2221 tgctggccat ctacctgcat gctggcccca ctgcccacga gctggccgtg gctgccgtgg 2281 acctgctgaa ccgccacgcc accgaatttg atgcagccca ggtgctgcag atgctgccTG 2341 ACACCTGGTC AGTGCAGCTC CTCTGCCCAT TCCTGATGGG GGCCATGAGG GACAGCATCC 2401 ATGCCAGGAG GACCATGCAG GTGGCTCTCG GCCTGGCCAG GTCCGAAAAC TTAATCTACA 2461 CCTACGATAA GATGAAGTTG AAAGGAAGCT CAATCCAACT CTCAGACAAA AAGCTTTGTC 2521 AGATATGCCA AAATCCCTTT TGTGAGCCTG TGTTTGTTAG ATACCCAAAT GGTGGTCTTG 2581 TGCACACCCA CTGTGCCGCC AGCAGACACA CAAACCCCAG CTCATCCAGT CCTGGCACTC 2641 GGACTTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 2701 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 2761 CTTGGCTTTA TATATCTTGT GGAAAGGACG AACCCTTTCA GGCTTCGACC GAGTTACGCG 2821 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt