Construct: ORF TRCN0000467359
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000275.1_s317c1
- Derived from:
- BRDN0000393861
- DNA Barcode:
- GCGGCTCATTGTCAACTTTCAGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FOXP4 (116113)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000467359
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 116113 | FOXP4 | forkhead box P4 | NM_138457.3 | 99.7% | 99.7% | 21_23delGGAinsCCT;1244_1245delGCinsAA |
| 2 | human | 116113 | FOXP4 | forkhead box P4 | XM_024446319.1 | 99.6% | 99.5% | 21_23delGGAinsCCT;657_659delAGC;1247_1248delGCinsAA |
| 3 | human | 116113 | FOXP4 | forkhead box P4 | XM_011514291.3 | 98.5% | 98.5% | 21_23delGGAinsCCT;657_680del;1268_1269delGCinsAA |
| 4 | human | 116113 | FOXP4 | forkhead box P4 | XM_006714991.3 | 97.9% | 97.9% | 21_23delGGAinsCCT;1147_1182del;1280_1281delGCinsAA |
| 5 | human | 116113 | FOXP4 | forkhead box P4 | NM_001012426.1 | 97.8% | 97.7% | (many diffs) |
| 6 | human | 116113 | FOXP4 | forkhead box P4 | NM_001012427.2 | 97.5% | 97.5% | (many diffs) |
| 7 | human | 116113 | FOXP4 | forkhead box P4 | XM_011514289.2 | 96.8% | 96.7% | (many diffs) |
| 8 | human | 116113 | FOXP4 | forkhead box P4 | XM_017010233.1 | 96.8% | 96.7% | (many diffs) |
| 9 | human | 116113 | FOXP4 | forkhead box P4 | XM_011514290.2 | 96.5% | 96.5% | (many diffs) |
| 10 | human | 116113 | FOXP4 | forkhead box P4 | XM_011514292.3 | 92.1% | 92.1% | (many diffs) |
| 11 | human | 116113 | FOXP4 | forkhead box P4 | XM_017010234.2 | 89.6% | 90.1% | (many diffs) |
| 12 | human | 116113 | FOXP4 | forkhead box P4 | XM_011514293.3 | 81.5% | 81.5% | (many diffs) |
| 13 | human | 116113 | FOXP4 | forkhead box P4 | XR_926052.3 | 51.9% | (many diffs) | |
| 14 | human | 116113 | FOXP4 | forkhead box P4 | XR_926053.2 | 50% | (many diffs) | |
| 15 | mouse | 74123 | Foxp4 | forkhead box P4 | NM_028767.2 | 87.6% | 91.8% | (many diffs) |
| 16 | mouse | 74123 | Foxp4 | forkhead box P4 | NM_001110825.1 | 87.5% | 91.6% | (many diffs) |
| 17 | mouse | 74123 | Foxp4 | forkhead box P4 | XM_030250108.1 | 86.1% | 90.2% | (many diffs) |
| 18 | mouse | 74123 | Foxp4 | forkhead box P4 | NM_001110824.1 | 86% | 90% | (many diffs) |
| 19 | mouse | 74123 | Foxp4 | forkhead box P4 | XM_030250107.1 | 86% | 90% | (many diffs) |
| 20 | mouse | 74123 | Foxp4 | forkhead box P4 | XM_017317688.2 | 85.2% | 89.3% | (many diffs) |
| 21 | mouse | 74123 | Foxp4 | forkhead box P4 | XM_017317686.2 | 85.1% | 89.1% | (many diffs) |
| 22 | mouse | 74123 | Foxp4 | forkhead box P4 | XM_017317687.2 | 85.1% | 89.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2067
- ORF length:
- 2001
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgat ggtggaatct gcctccctga caatcaggtc ggctccatct ggtcagaatg 121 gcgtgggcag cctctctggg caagccgatg gcagcagcgg cggggccaca gggacaactg 181 caagtggcac gggcagggaa gtgaccacgg gtgcagacag caatggtgag atgagtcccg 241 cagagctgct gcacttccag cagcaacagg ctctccaagt ggcccggcag ttcctgctgc 301 agcaggcctc aggcctgagc tccccaggga acaatgacag caaacagtct gcctctgctg 361 tgcaggtgcc tgtgtcggtg gccatgatgt cgccgcagat gcttaccccg caacagatgc 421 agcagatcct gtcgcccccg cagctgcagg ccttgctcca gcagcagcaa gccctcatgc 481 tccagcagct acaggagtac tacaagaagc agcaggagca gctccacctg cagctcctca 541 cccagcagca ggctgggaaa ccgcagccca aagaggcact ggggaacaag cagctggcct 601 tccagcagca gctcctgcaa atgcaacagt tgcagcagca gcacctgctc aacctgcaga 661 ggcaggggct ggtcagcctg cagcccaacc aagcctcggg gcccctccag acccttccgc 721 aagctgtttg cccaacagac ctgccccagc tgtggaaggg cgagggtgcc cccgggcagc 781 ctgccgagga cagcgtcaag caggaggggc tggacctcac tggcacggcc gccaccgcta 841 cctcgtttgc cgctcccccc aaggtctcac cccccctctc ccaccatacc ctgcccaacg 901 gacagcctac tgtgctcaca tctcggagag acagctcttc ccacgaggag acccccggct 961 cccaccccct gtacggacac ggagagtgca agtggccagg ctgtgagacc ctgtgtgaag 1021 acctgggcca gtttatcaaa cacctcaaca cagagcacgc cctggatgac cggagtacag 1081 cccagtgccg ggtacagatg caggtggtgc agcagctgga gatccagctc gccaaggaga 1141 gcgagcggct gcaggccatg atggcccacc tgcacatgcg gccctcggag cccaagccct 1201 tcagccagcc agtgaccgtc tctgcagcag actcattccc agatggtctc gtgcaccccc 1261 cgacctcggc cgcagcccct gtcacccctc tacggccccc tggcctggaa tctgcctccc 1321 tgcatggtgg gggcccagcc cgtcggagaa gcagtgacaa gttctgctcc cccatctcct 1381 cagagctggc ccagaatcat gagttctaca agaacgccga cgtccggccc cccttcacct 1441 acgcctccct catccgccag gccatcctgg aaacccctga caggcagctg accctgaatg 1501 agatctataa ctggttcacc aggatgttcg cctatttccg cagaaacact gccacctgga 1561 agaacgccgt gcgccacaac ctcagcctgc acaagtgctt cgtccgcgtg gagaacgtca 1621 agggtgccgt gtggactgtg gacgagcggg agtatcagaa gcggagaccg ccaaagatga 1681 cagggagccc caccctggtg aagaacatga tctctggccT CAGCTATGGA GCACTTAATG 1741 CCAGCTACCA GGCCGCCCTG GCCGAGAGCA GCTTCCCCCT CCTCAACAGC CCTGGCATGC 1801 TGAACCCTGG CTCCGCCAGC AGCCTGCTGC CCCTCAGCCA CGATGACGTG GGTGCCCCCG 1861 TGGAGCCGCT GCCCAGCAAC GGCAGCAGCA GCCCTCCTCG CCTCTCCCCG CCCCAGTACA 1921 GCCACCAGGT GCAGGTGAAG GAGGAGCCAG CAGAGGCAGA GGAAGACAGG CAGCCCGGGC 1981 CTCCCCTGGG CGCCCCTAAC CCCAGCGCCT CGGGGCCTCC GGAAGACAGG GACCTGGAGG 2041 AGGAGCTGCC GGGAGAAGAA CTGTCCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG 2101 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 2161 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCGGCTCA 2221 TTGTCAACTT TCAGGTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 2281 aagatt