Construct: ORF TRCN0000468757
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015896.1_s317c1
- Derived from:
- BRDN0000395080
- DNA Barcode:
- CAAGGTTGCAACAGACTCTACTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFPM2 (23414)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000468757
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23414 | ZFPM2 | zinc finger protein, FOG fa... | NM_012082.4 | 99.8% | 99.7% | 20_22delGCAinsAGG;2917_2918delAAinsGC |
| 2 | human | 23414 | ZFPM2 | zinc finger protein, FOG fa... | XM_011516947.3 | 98.8% | 98.7% | (many diffs) |
| 3 | human | 23414 | ZFPM2 | zinc finger protein, FOG fa... | NM_001362836.2 | 95.2% | 95.1% | 20_22delGCAinsAGG;40_41ins159;2758_2759delAAinsGC |
| 4 | human | 23414 | ZFPM2 | zinc finger protein, FOG fa... | NM_001362837.2 | 88.4% | 88.4% | 0_1ins396;2521_2522delAAinsGC |
| 5 | mouse | 22762 | Zfpm2 | zinc finger protein, multit... | NM_011766.5 | 88.9% | 93.6% | (many diffs) |
| 6 | mouse | 22762 | Zfpm2 | zinc finger protein, multit... | XM_006520897.2 | 85.1% | 90% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3522
- ORF length:
- 3453
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtcccggcga aagcaaaagg aaccccggca gatcaaacgg ccgcttgaag 121 atgccattga agatgaggaa gaagaatgtc catcagagga aacagacatc atctccaaag 181 gagactttcc attggaggaa agcttttcca cagaatttgg gcctgaaaat ctgagctgcg 241 aagaagtgga atacttttgt aacaaaggtg atgatgaagg aatccaggag acagcagaat 301 cagatgggga cacacagtca gagaaaccgg ggcaacctgg agttgagaca gacgactggg 361 atggaccagg agagctggag gtgtttcaga aagatgggga acgaaaaatt cagagtcgac 421 agcaacttcc agtgggaaca acctgggggc cgtttcctgg gaagatggac ttgaataata 481 attctttgaa gacaaaggct caggtcccaa tggtgctgac tgctggtccc aagtggttgc 541 tggatgtgac ttggcaagga gtggaagaca acaaaaacaa ctgcattgtg tacagcaaag 601 ggggtcagct ttggtgtaca actacgaagg ccatctctga gggtgaagag ctaattgcct 661 ttgtggtgga ttttgactca aggctacaag ctgccagtca gatgactctc acagaaggga 721 tgtaccctgc acgcctgctg gactcaattc agctgcttcc tcagcaagct gccatggctt 781 ctattttgcc cacagctatt gtcaataagg atatattccc ttgcaagtcc tgtggcatct 841 ggtatcggag tgagcggaat ctgcaggccc atttgatgta ctactgcagt gggaggcaaa 901 gagaagctgc tccggtgtca gaggaaaatg aagacagtgc ccatcagatt tccagcctgt 961 gccccttccc acagtgcacc aagagctttt caaatgctcg agctctagaa atgcacctga 1021 attcacacag tggagtgaaa atggaagaat tcctgccccc tggtgctagt ctaaaatgca 1081 ccgtctgtag ctacactgct gattccgtga tcaactttca ccaacacctg ttctcccatc 1141 tcactcaagc tgccttccga tgtaatcact gccatttcgg cttccagact cagagggagt 1201 tattgcagca ccaggagctc catgtcccta gcggcaaact tcccagagaa agtgacatgg 1261 aacactctcc aagtgcaact gaagacagct tacagccagc cacagactta ttgaccagaa 1321 gcgaacttcc ccagagccaa aaggccatgc agactaaaga tgcgagctct gacacagagc 1381 tggacaagtg tgagaaaaag actcagctct ttctcacgaa ccagagacca gagatacagc 1441 ctacaacaaa taaacaaagc ttttcttaca caaaaataaa gtctgagccc tctagcccaa 1501 gacttgcctc atctccagtt cagcctaata ttgggccttc tttccctgtg ggccctttcc 1561 tatctcagtt ttctttcccc caagatatca ccatggtccc tcaagcttca gagatcttag 1621 ctaagatgtc tgaactggtg catcggcgac tgaggcatgg cagtagtagc taccctcccg 1681 tcatttacag ccctttgatg cccaaggggg ctacttgttt tgagtgtaac ataacattca 1741 ataatttgga taattatcta gtgcacaaaa agcattattg cagcagccga tggcagcaga 1801 tggctaagtc cccagagttc cctagtgtgt cagaaaagat gcctgaagct ttgagtccca 1861 acactggcca aacctccata aaccttctca acccagctgc tcattctgct gatcctgaga 1921 atccacttct tcaaacatct tgcatcaatt cttccactgt cttagattta attgggccaa 1981 atgggaaggg ccatgacaag gacttttcca ctcaaactaa gaagctctcc acctccagta 2041 acaatgatga caaaattaat ggaaaacctg ttgatgtgaa aaatcccagt gtccccttag 2101 tggatgggga aagtgaccca aataagacta cctgtgaagc ttgcaacatt accttcagcc 2161 ggcacgaaac atacatggtc cacaaacagt attactgtgc tacacgccac gaccctccac 2221 tgaagaggtc tgcttccaac aaagtgcctg ccatgcagag aaccatgcgc acacgcaagc 2281 gcagaaagat gtatgagatg tgcctacctg agcaggaaca aaggcctcca ctggttcagc 2341 agagatttct tgacgtagcc aacctcaata atccttgtac ctccactcaa gaacccacag 2401 aagggctagg agagtgctac cacccaagat gtgatatctt tccaggaatt gtctctaaac 2461 acttggaaac ttctctgacg atcaacaagt gtgttccagt ttccaaatgt gatactactc 2521 attccagtgt ttcctgccta gagatggacg tgcccataga tctcagcaaa aagtgtttat 2581 ctcagtctga gcggacgacc acgtctccca aaaggctgct ggactatcac gagtgcactg 2641 tgtgcaagat cagtttcaat aaggtagaaa actatctggc ccacaagcag aatttctgcc 2701 cggttactgc acatcagcgt aatgacctgg gtcaactgga cggcaaagtg tttccgaatc 2761 cagaaagcga acgaaacagc cctgatgtca gctacgaaag aagcataata aaatgtgaga 2821 aaaatgggaa tttgaagcag ccttccccca atggaaactt attttcatcc cacctagcaa 2881 ccctgcaagg cttgaaggtc tttagtgaag ctgctcagct cattgctaca aaagaagaaa 2941 acagacattt gtttcttcca caatgccttt accctggagc aatagcgaaa gcaaaaggag 3001 ccgaccagct ttctccatat tatggaatca agccaagtga ttatatttct ggttctcttg 3061 tcatccataa cactgacatc gagcaaagca gaaatgcaga aaatgaatct ccTAAAGGCC 3121 AGGCTTCCTC AAATGGGTGT GCTGCGCTGA AGAAAGATTC TCTGCCATTG TTGCCCAAAA 3181 ATCGAGGAAT GGTAATAGTG AATGGTGGAC TGAAACAAGA TGAGAGACCT GCTGCCAACC 3241 CACAGCAAGA GAACATTTCC CAGAATCCTC AGCACGAAGA CGACCACAAA TCTCCCTCGT 3301 GGATCTCTGA GAACCCATTA GCTGCCAATG AGAATGTCTC ACCAGGAATT CCCTCAGCAG 3361 AGGAACAGTT GTCTAGTATA GCAAAAGGTG TGAATGGTTC CAGCCAGGCT CCAACCAGTG 3421 GGAAATATTG CCGGCTATGT GATATCCAGT TCAACAACCT TTCAAACTTT ATAACTCACA 3481 AGAAGTTTTA TTGCTCATCA CATGCAGCAG AACATGTCAA ATTGCCAACT TTCTTGTACA 3541 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 3601 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 3661 AGGACGACAA GGTTGCAACA GACTCTACTT TACGCGTTAA GTCgacaatc aacctctgga 3721 ttacaaaatt tgtgaaagat t