Construct: ORF TRCN0000469015

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF013173.1_s317c1
Derived from:
ccsbBroadEn_09020
DNA Barcode:
GGAGGAGGTCTCCTAAGCCCCTAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RUFY1 (80230)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469015
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 80230 RUFY1 RUN and FYVE domain contain... NM_001040451.3 99.9% 100% 1218G>A
2 human 80230 RUFY1 RUN and FYVE domain contain... NM_001040452.3 99.9% 100% 1218G>A
3 human 80230 RUFY1 RUN and FYVE domain contain... XM_017009891.1 96.2% 96.1% 566_567ins66;1152G>A
4 human 80230 RUFY1 RUN and FYVE domain contain... NM_025158.5 84.6% 84.7% 1_324del;1542G>A
5 human 80230 RUFY1 RUN and FYVE domain contain... XM_006714921.3 81.5% 81.4% 1_324del;890_891ins66;1476G>A
6 human 80230 RUFY1 RUN and FYVE domain contain... XM_005265993.4 77.4% 77.5% 0_1ins405;813G>A
7 human 80230 RUFY1 RUN and FYVE domain contain... XR_245277.3 77% (many diffs)
8 human 80230 RUFY1 RUN and FYVE domain contain... XM_024446220.1 72.5% 71.9% (many diffs)
9 human 80230 RUFY1 RUN and FYVE domain contain... XM_017009893.2 68.9% 68.1% (many diffs)
10 human 80230 RUFY1 RUN and FYVE domain contain... XR_001742278.1 64.6% (many diffs)
11 human 80230 RUFY1 RUN and FYVE domain contain... XR_245276.3 63.7% (many diffs)
12 human 80230 RUFY1 RUN and FYVE domain contain... XR_001742279.1 62.2% (many diffs)
13 human 80230 RUFY1 RUN and FYVE domain contain... XM_006714922.3 61.2% 61.1% (many diffs)
14 human 80230 RUFY1 RUN and FYVE domain contain... XM_017009894.1 61.2% 61.1% (many diffs)
15 human 80230 RUFY1 RUN and FYVE domain contain... XM_017009895.2 51.6% 51.6% 0_1ins870;348G>A
16 mouse 216724 Rufy1 RUN and FYVE domain contain... XM_006532841.3 85.5% 91.9% (many diffs)
17 mouse 216724 Rufy1 RUN and FYVE domain contain... NM_172557.2 74.3% 79.9% (many diffs)
18 mouse 216724 Rufy1 RUN and FYVE domain contain... XR_001779949.1 47.9% (many diffs)
19 mouse 216724 Rufy1 RUN and FYVE domain contain... XM_017314442.1 32.6% 32.8% (many diffs)
20 mouse 216724 Rufy1 RUN and FYVE domain contain... XR_001779948.1 30.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1866
ORF length:
1800
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat ggaggagcgt gccaacctga tgcacatgat gaaactcagc atcaaggtgt
121tgctccagtc ggctctgagc ctgggccgca gcctggatgc ggaccatgcc cccttgcagc
181agttctttgt agtgatggag cactgcctca aacatgggct gaaagttaag aagagtttta
241ttggccaaaa taaatcattc tttggtcctt tggagctggt ggagaaactt tgtccagaag
301catcagatat agcgactagt gtcagaaatc ttccagaatt aaagacagct gtgggaagag
361gccgagcgtg gctttatctt gcactcatgc aaaagaaact ggcagattat ctgaaagtgc
421ttatagacaa taaacatctc ttaagcgagt tctatgagcc tgaggcttta atgatggagg
481aagaagggat ggtgattgtt ggtctgctgg tgggactcaa tgttctcgat gccaatctct
541gcttgaaagg agaagacttg gattctcagg ttggagtaat agatttttcc ctctacctta
601aggatgtgca ggatcttgat ggtggcaagg agcatgaaag aattactgat gtccttgatc
661aaaaaaatta tgtggaagaa cttaaccggc acttgagctg cacagttggg gatcttcaaa
721ccaagataga tggcttggaa aagactaact caaagcttca agaagagctt tcagctgcaa
781cagaccgaat ttgctcactt caagaagaac agcagcagtt aagagaacaa aatgaattaa
841ttcgagaaag aagtgaaaag agtgtagaga taacaaaaca ggataccaaa gttgagctgg
901agacttacaa gcaaactcgg caaggtctgg atgaaatgta cagtgatgtg tggaagcagc
961taaaagagga gaagaaagtc cggttggaac tggaaaaaga actggagtta caaattggaa
1021tgaaaaccga aatggaaatt gcaatgaagt tactggaaaa ggacacccac gagaagcagg
1081acacactagt tgccctccgc cagcagctgg aagaagtcaa agcgattaat ttacagatgt
1141ttcacaaagc tcagaatgca gagagcagtt tgcagcagaa gaatgaagcc atcacatcct
1201ttgaaggaaa aaccaaccaa gttatgtcca gcatgaaaca aatggaagaa aggttgcagc
1261actcggagcg ggcgaggcag ggagctgagg agcggagcca caagctgcag caggagctgg
1321gcgggaggat cggcgccctg cagctgcagc tctcccagct gcacgagcaa tgctcaagcc
1381tggagaaaga attgaaatca gaaaaagagc aaagacaggc tcttcagcgc gaattacagc
1441acgagaaaga cacttcctct ctactcagga tggagctgca acaagtggaa ggactgaaaa
1501aggagttgcg ggagcttcag gacgagaagg cagagctgca gaagaTCTGC GAGGAGCAGG
1561AACAAGCCCT CCAGGAAATG GGCCTGCACC TCAGCCAGTC CAAGCTGAAG ATGGAAGATA
1621TAAAAGAAGT GAACCAGGCA CTGAAGGGCC ACGCCTGGCT GAAAGATGAC GAAGCGACAC
1681ACTGTAGGCA GTGTGAGAAG GAGTTCTCCA TTTCCCGGAG AAAGCACCAC TGCCGGAACT
1741GTGGCCACAT CTTCTGCAAC ACCTGCTCCA GCAACGAGCT GGCCCTGCCC TCCTACCCCA
1801AGCCGGTGCG AGTGTGCGAC AGCTGCCACA CCCTGCTCCT GCAGCGCTGC TCCTCCACGG
1861CCTCCTGCCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC
1921TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT
1981CTTGGCTTTA TATATCTTGT GGAAAGGACG AGGAGGAGGT CTCCTAAGCC CCTAGACGCG
2041TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt

Download FASTA (ORF) (Full)