Construct: ORF TRCN0000469479
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008436.1_s317c1
- Derived from:
- ccsbBroadEn_10208
- DNA Barcode:
- CACTGCACGGTTCACGCTGAGAAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- IGF2BP2 (10644)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469479
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10644 | IGF2BP2 | insulin like growth factor ... | NM_006548.6 | 99.8% | 99.8% | 1_3delATG |
| 2 | human | 10644 | IGF2BP2 | insulin like growth factor ... | NM_001291869.3 | 98.8% | 98.8% | 1_3delATG;339_356del |
| 3 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_017005557.2 | 93.7% | 89.7% | 1_3delATG;177_178ins61;277_328del |
| 4 | human | 10644 | IGF2BP2 | insulin like growth factor ... | NM_001007225.3 | 92.6% | 92.6% | 1_3delATG;1071_1072ins129 |
| 5 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_017005558.2 | 91.7% | 91.7% | 1_3delATG;339_356del;1089_1090ins129 |
| 6 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_017005559.2 | 91.5% | 91.5% | 1_3delATG;339_356del;1479_1480ins132 |
| 7 | human | 10644 | IGF2BP2 | insulin like growth factor ... | NM_001291873.2 | 88.1% | 86.7% | (many diffs) |
| 8 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_017005560.2 | 87.9% | 87.9% | 0_1ins201;135_152del |
| 9 | human | 10644 | IGF2BP2 | insulin like growth factor ... | NM_001291872.3 | 87.2% | 85.8% | (many diffs) |
| 10 | human | 10644 | IGF2BP2 | insulin like growth factor ... | NM_001291874.3 | 80.9% | 79.6% | (many diffs) |
| 11 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_017005561.1 | 78.9% | 77% | (many diffs) |
| 12 | human | 10644 | IGF2BP2 | insulin like growth factor ... | NM_001291875.3 | 77.4% | 77.4% | 0_1ins405 |
| 13 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_024453316.1 | 77.4% | 77.4% | 0_1ins405 |
| 14 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_017005562.1 | 76.9% | 76.1% | (many diffs) |
| 15 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XR_001739984.2 | 61.2% | 1_88del;424_441del;1901_2929del | |
| 16 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_017005563.1 | 60.4% | 59.6% | (many diffs) |
| 17 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_017005564.1 | 60.4% | 59.6% | (many diffs) |
| 18 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_006713477.4 | 57.3% | 57.3% | 0_1ins765 |
| 19 | human | 10644 | IGF2BP2 | insulin like growth factor ... | XM_006713478.4 | 50.1% | 50.1% | 0_1ins765;303_304ins129 |
| 20 | human | 10644 | IGF2BP2 | insulin like growth factor ... | NR_138486.2 | 47.1% | 1_85del;1153_1154ins129;1751_3400del | |
| 21 | mouse | 319765 | Igf2bp2 | insulin-like growth factor ... | NM_183029.2 | 89.7% | 94.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1860
- ORF length:
- 1794
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa caagctttac atcgggaacc tgagccccgc cgtcaccgcc gacgacctcc 121 ggcagctctt tggggacagg aagctgcccc tggcgggaca ggtcctgctg aagtccggct 181 acgccttcgt ggactacccc gaccagaact gggccatccg cgccatcgag accctctcgg 241 gtaaagtgga attgcatggg aaaatcatgg aagttgatta ctcagtctct aaaaagctaa 301 ggagcaggaa aattcagatt cgaaacatcc ctcctcacct gcagtgggag gtgttggatg 361 gacttttggc tcaatatggg acagtggaga atgtggaaca agtcaacaca gacacagaaa 421 ccgccgttgt caacgtcaca tatgcaacaa gagaagaagc aaaaatagcc atggagaagc 481 taagcgggca tcagtttgag aactactcct tcaagatttc ctacatcccg gatgaagagg 541 tgagctcccc ttcgccccct cagcgagccc agcgtgggga ccactcttcc cgggagcaag 601 gccacgcccc tgggggcact tctcaggcca gacagattga tttcccgctg cggatcctgg 661 tccccaccca gtttgttggt gccatcatcg gaaaggaggg cttgaccata aagaacatca 721 ctaagcagac ccagtcccgg gtagatatcc atagaaaaga gaactctgga gctgcagaga 781 agcctgtcac catccatgcc accccagagg ggacttctga agcatgccgc atgattcttg 841 aaatcatgca gaaagaggca gatgagacca aactagccga agagattcct ctgaaaatct 901 tggcacacaa tggcttggtt ggaagactga ttggaaaaga aggcagaaat ttgaagaaaa 961 ttgaacatga aacagggacc aagataacaa tctcatcttt gcaggatttg agcatataca 1021 acccggaaag aaccatcact gtgaagggca cagttgaggc ctgtgccagt gctgagatag 1081 agattatgaa gaagctgcgt gaggcctttg aaaatgatat gctggctgtt aaccaacaag 1141 ccaatctgat cccagggttg aacctcagcg cacttggcat cttttcaaca ggactgtccg 1201 tgctatctcc accagcaggg ccccgcggag ctccccccgc tgccccctac caccccttca 1261 ctacccactc cggatacttc tccagcctgt acccccatca ccagtttggc ccgttcccgc 1321 atcatcactc ttatccagag caggagattg tgaatctctt catcccaacc caggctgtgg 1381 gcgccatcat cgggaagaag ggggcacaca tcaaacagct ggcgagattc gccggagcct 1441 ctatcaagat tgccccTGCG GAAGGCCCAG ACGTCAGCGA AAGGATGGTC ATCATCACCG 1501 GGCCACCGGA AGCCCAGTTC AAGGCCCAGG GACGGATCTT TGGGAAACTG AAAGAGGAAA 1561 ACTTCTTTAA CCCCAAAGAA GAAGTGAAGC TGGAAGCGCA TATCAGAGTG CCCTCTTCCA 1621 CAGCTGGCCG GGTGATTGGC AAAGGTGGCA AGACCGTGAA CGAACTGCAG AACTTAACCA 1681 GTGCAGAAGT CATCGTGCCT CGTGACCAAA CGCCAGATGA AAATGAGGAA GTGATCGTCA 1741 GAATTATCGG GCACTTCTTT GCTAGCCAGA CTGCACAGCG CAAGATCAGG GAAATTGTAC 1801 AACAGGTGAA GCAGCAGGAG CAGAAATACC CTCAGGGAGT CGCCTCACAG CGCAGCAAGT 1861 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 1921 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1981 TTTATATATC TTGTGGAAAG GACGACACTG CACGGTTCAC GCTGAGAAAA CGCGTTAAGT 2041 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt