Construct: ORF TRCN0000469531
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013915.1_s317c1
- Derived from:
- ccsbBroadEn_11778
- DNA Barcode:
- TGATGTTGGCGCACACCTGGGAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- POC1A (25886)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469531
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25886 | POC1A | POC1 centriolar protein A | NM_001161581.2 | 98.6% | 98.6% | 1_15del |
| 2 | human | 25886 | POC1A | POC1 centriolar protein A | NM_015426.5 | 89.4% | 89.4% | 1_129del |
| 3 | human | 25886 | POC1A | POC1 centriolar protein A | XM_017006104.1 | 85.6% | 85.6% | 1_15del;866_867ins144 |
| 4 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533560.1 | 81.3% | 81.3% | 1_129del;882_883ins99 |
| 5 | human | 25886 | POC1A | POC1 centriolar protein A | NM_001161580.2 | 77.6% | 77.6% | 1_129del;980_981ins144 |
| 6 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533561.1 | 69.5% | 69.5% | 1_129del;882_883ins243 |
| 7 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533562.2 | 62.9% | 61.9% | (many diffs) |
| 8 | human | 25886 | POC1A | POC1 centriolar protein A | XR_001740088.1 | 51% | 1_169del;1021_1262del;1504_2140del | |
| 9 | human | 25886 | POC1A | POC1 centriolar protein A | XR_940402.1 | 50.4% | 1_169del;719_720ins134;1128_1764del | |
| 10 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533563.1 | 49.8% | 44.7% | 1_129del;679_680ins134;738_739ins349 |
| 11 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533564.3 | 49.8% | 44.7% | 1_129del;679_680ins134;738_739ins349 |
| 12 | human | 25886 | POC1A | POC1 centriolar protein A | XM_011533565.2 | 49.8% | 44.7% | 1_129del;679_680ins134;738_739ins349 |
| 13 | human | 25886 | POC1A | POC1 centriolar protein A | XR_940403.1 | 42.8% | (many diffs) | |
| 14 | mouse | 70235 | Poc1a | POC1 centriolar protein A | NM_027354.2 | 79.3% | 81% | (many diffs) |
| 15 | mouse | 70235 | Poc1a | POC1 centriolar protein A | XM_006511830.3 | 69.5% | 64.6% | (many diffs) |
| 16 | mouse | 70235 | Poc1a | POC1 centriolar protein A | XM_006511831.3 | 67.2% | 66.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1158
- ORF length:
- 1092
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ctggcacatg aagccgcagt cacgcgccta ccgcttcact ggccacaagg 121 atgccgtcac ctgtgtgaac ttctctcctt cgggacacct gcttgcttcc ggctcccgag 181 acaagactgt ccgcatctgg gtacccaatg tcaaaggtga gtccactgtg tttcgtgcac 241 acacagccac agtgaggagt gtccacttct gcagtgatgg ccagtccttc gtgacagcct 301 ctgacgacaa gacagtcaaa gtgtgggcaa ctcatcgcca gaaattcctg ttctccctga 361 gccagcatat caactgggtc cgctgtgcca agttctcccc cgacgggcgg ctcatcgtgt 421 ctgccagtga tgacaagact gttaagctgt gggacaagag cagccgggaa tgtgtccact 481 cgtattgtga gcatggcggc tttgtcacct atgtggactt ccaccccagt gggacgtgca 541 ttgccgctgc cggcatggac aacacagtga aggtgtggga cgtgcggact caccggctgc 601 tgcagcatta tcagttgcac agtgcagcag tgaacgggct ctctttccac ccGTCGGGAA 661 ACTACCTGAT CACAGCCTCC AGTGACTCAA CCCTGAAGAT CCTGGACCTG ATGGAGGGCC 721 GGCTGCTCTA CACACTCCAC GGGCATCAGG GACCAGCCAC CACTGTTGCC TTTTCAAGAA 781 CGGGGGAGTA TTTTGCTTCT GGAGGCTCTG ATGAACAAGT GATGGTTTGG AAGAGTAACT 841 TTGATATTGT TGATCATGGA GAAGTCACGA AAGTGCCGAG GCCCCCAGCC ACACTGGCCA 901 GCTCCATGGG GAATCTGCCA GAAGTGGACT TCCCTGTCCC CCCAGGCAGA GGCAGGAGTG 961 TGGAGTCTGT GCAGAGCCAG CCCCAGGAGC CCGTGAGTGT GCCCCAGACA CTGACTAGCA 1021 CGCTGGAGCA CATTGTGGGC CAGCTGGATG TCCTCACTCA GACAGTCTCC ATTCTGGAGC 1081 AGCGGTTGAC ACTGACAGAA GACAAGCTGA AGCAGTGTCT GGAGAACCAG CAGCTAATCA 1141 TGCAGAGAGC AACACCATGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1201 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1261 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGATGTT GGCGCACACC 1321 TGGGAACACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt