Construct: ORF TRCN0000469993
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018828.2_s317c1
- Derived from:
- ccsbBroadEn_14715
- DNA Barcode:
- CCAAGGCATCGCGTCTCAAAGTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NME2 (4831)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469993
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018137.3 | 100% | 100% | |
| 2 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018138.1 | 100% | 100% | |
| 3 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001018139.2 | 100% | 100% | |
| 4 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_002512.4 | 100% | 100% | |
| 5 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_000269.2 | 81.4% | 88.1% | (many diffs) |
| 6 | human | 4830 | NME1 | NME/NM23 nucleoside diphosp... | NM_198175.1 | 69.9% | 75.7% | (many diffs) |
| 7 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NM_001018136.3 | 56.9% | 56.9% | 1_345del |
| 8 | human | 4831 | NME2 | NME/NM23 nucleoside diphosp... | NM_001198682.2 | 53.9% | 49.6% | 227_228ins113;246_247ins97 |
| 9 | human | 654364 | NME1-NME2 | NME1-NME2 readthrough | NR_037149.2 | 35.8% | 1_673del;1130_1272del | |
| 10 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_001077529.2 | 90.3% | 98% | (many diffs) |
| 11 | mouse | 18103 | Nme2 | NME/NM23 nucleoside diphosp... | NM_008705.5 | 90.3% | 98% | (many diffs) |
| 12 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | NM_008704.2 | 82.7% | 90.1% | (many diffs) |
| 13 | mouse | 18102 | Nme1 | NME/NM23 nucleoside diphosp... | XM_030245646.1 | 75.7% | 82.5% | (many diffs) |
| 14 | mouse | 433968 | Gm5566 | predicted pseudogene 5566 | XR_868740.1 | 69.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 525
- ORF length:
- 456
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggccaacctg gagcgcacct tcatcgccat caagccggac ggcgtgcagc 121 gcggcctggt gggcgagatc atcaagcgct tcgagcagaa gggattccgc ctcgtggcca 181 tgaagttccT CCGGGCCTCT GAAGAACACC TGAAGCAGCA CTACATTGAC CTGAAAGACC 241 GACCATTCTT CCCTGGGCTG GTGAAGTACA TGAACTCAGG GCCGGTTGTG GCCATGGTCT 301 GGGAGGGGCT GAACGTGGTG AAGACAGGCC GAGTGATGCT TGGGGAGACC AATCCAGCAG 361 ATTCAAAGCC AGGCACCATT CGTGGGGACT TCTGCATTCA GGTTGGCAGG AACATCATTC 421 ATGGCAGTGA TTCAGTAAAA AGTGCTGAAA AAGAAATCAG CCTATGGTTT AAGCCTGAAG 481 AACTGGTTGA CTACAAGTCT TGTGCTCATG ACTGGGTCTA TGAATTGCCA ACTTTCTTGT 541 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 601 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 661 GAAAGGACGA CCAAGGCATC GCGTCTCAAA GTAAACGCGT TAAGTCgaca atcaacctct 721 ggattacaaa atttgtgaaa gatt