Construct: ORF TRCN0000470729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013484.1_s317c1
- Derived from:
- ccsbBroadEn_12929
- DNA Barcode:
- AAAAGCGCGGATTCACTAAACTAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PAPLN (89932)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470729
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89932 | PAPLN | papilin, proteoglycan like ... | XR_429333.3 | 13% | (many diffs) | |
| 2 | human | 89932 | PAPLN | papilin, proteoglycan like ... | XM_011537296.2 | 12.5% | 12.1% | (many diffs) |
| 3 | human | 89932 | PAPLN | papilin, proteoglycan like ... | XM_017021749.2 | 10.9% | 10.6% | (many diffs) |
| 4 | human | 89932 | PAPLN | papilin, proteoglycan like ... | XM_011537295.3 | 10.4% | 10.1% | (many diffs) |
| 5 | human | 89932 | PAPLN | papilin, proteoglycan like ... | XM_011537293.3 | 10.2% | 9.9% | (many diffs) |
| 6 | human | 89932 | PAPLN | papilin, proteoglycan like ... | NM_173462.4 | 10.1% | 9.8% | (many diffs) |
| 7 | human | 89932 | PAPLN | papilin, proteoglycan like ... | NM_001365907.2 | 10% | 9.7% | (many diffs) |
| 8 | human | 89932 | PAPLN | papilin, proteoglycan like ... | NM_001365906.2 | 9.9% | 9.6% | (many diffs) |
| 9 | human | 89932 | PAPLN | papilin, proteoglycan like ... | XM_011537290.3 | 9.9% | 9.6% | (many diffs) |
| 10 | human | 89932 | PAPLN | papilin, proteoglycan like ... | XM_011537291.3 | 9.9% | 9.6% | (many diffs) |
| 11 | human | 89932 | PAPLN | papilin, proteoglycan like ... | XM_011537292.3 | 9.9% | 9.6% | (many diffs) |
| 12 | human | 89932 | PAPLN | papilin, proteoglycan like ... | NR_158677.2 | 6.9% | (many diffs) | |
| 13 | human | 89932 | PAPLN | papilin, proteoglycan like ... | NR_158678.2 | 6.8% | (many diffs) | |
| 14 | human | 89932 | PAPLN | papilin, proteoglycan like ... | XM_011537294.3 | 6.1% | 5.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 477
- ORF length:
- 387
- Sequence:
-
1 TCAGACAGTG GTTCAAAGTT TTTTTCTTCC ATTTCAGGTG TCGTGAGGCT AGCATCGATT 61 GATCAACAAG TTTGTACAAA AAAGTTGGCA TGCGGCTGCT CCTGCTCGTG CCGCTGCTGC 121 TGGCTCCAGC GCCCGGGTCC TCGGCTCCCA AGGTGAGGCG GCAGAGTGAC ACCTGGGGAC 181 CCTGGAGCCA GTGGAGCCCC TGCAGCCGGA CCTGTGGAGG GGGTGTCAGC TTCCGGGAGC 241 GCCCCTGCTA CTCCCAGAGG AGAGATGGAG GCTCCAGCTG CGTGGGCCCC GCCCGGAGCC 301 ACCGCTCTTG TCGCACGGAG AGCTGCCCCG ACGGCGCCCG GGACTTCCGG GCCGAGCAGT 361 GCGCGGAGTT CGACGGAGCG GAGTTCCAGG GGCGGCGGTA TCGGTGGCTG CCCTACTACA 421 GCGCCCCAAA CAAGTGTGAA CTGAACTGCA TTCCCAAAGT GCTGGGATTA CAGGCATGCC 481 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 541 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 601 ATATATCTTG TGGAAAGGAC GAAAAAGCGC GGATTCACTA AACTACACGC GTTAAGTCga 661 caatcaacct ctggattaca aaatttgtga aagatt