Construct: ORF TRCN0000471116
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018941.3_s317c1
- Derived from:
- ccsbBroadEn_14615
- DNA Barcode:
- TTCGAAGCCCGGCTAAACTAAATC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DLG1 (1739)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471116
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001363865.1 | 98.4% | 23% | (many diffs) |
2 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366207.1 | 98.4% | 23% | (many diffs) |
3 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366210.1 | 98.4% | 23% | (many diffs) |
4 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366215.1 | 98.4% | 23% | (many diffs) |
5 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001204386.1 | 98.2% | 23% | (many diffs) |
6 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366205.1 | 98.2% | 23% | (many diffs) |
7 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366213.1 | 98.2% | 23% | (many diffs) |
8 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366211.1 | 96.4% | 23.3% | (many diffs) |
9 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366217.1 | 96.4% | 23.3% | (many diffs) |
10 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366206.1 | 96.4% | 21.1% | (many diffs) |
11 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005811.2 | 96.4% | 21.1% | (many diffs) |
12 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005812.1 | 96.4% | 21.1% | (many diffs) |
13 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005813.1 | 96.4% | 21.1% | (many diffs) |
14 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366220.1 | 96.3% | 21.1% | (many diffs) |
15 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366204.1 | 95.6% | 23.6% | (many diffs) |
16 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366208.1 | 95.6% | 23.6% | (many diffs) |
17 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366209.1 | 95.6% | 23.6% | (many diffs) |
18 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366212.1 | 95.6% | 23.6% | (many diffs) |
19 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005819.1 | 95.6% | 23.6% | (many diffs) |
20 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_004087.2 | 94.9% | 22.2% | (many diffs) |
21 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_005269289.4 | 94.9% | 22.2% | (many diffs) |
22 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005800.1 | 94.9% | 22.2% | (many diffs) |
23 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005801.1 | 94.9% | 22.2% | (many diffs) |
24 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005802.1 | 94.9% | 22.2% | (many diffs) |
25 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005803.1 | 94.9% | 22.2% | (many diffs) |
26 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366214.1 | 94.8% | 22.2% | (many diffs) |
27 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366203.1 | 94.5% | 21.4% | (many diffs) |
28 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366216.1 | 93.7% | 21.6% | (many diffs) |
29 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366219.1 | 93.7% | 21.6% | (many diffs) |
30 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001098424.1 | 92.3% | 22.7% | (many diffs) |
31 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001290983.1 | 92.3% | 22.7% | (many diffs) |
32 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366218.1 | 92.3% | 22.7% | (many diffs) |
33 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_011512502.3 | 92.3% | 22.7% | (many diffs) |
34 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005821.2 | 85.9% | 7.6% | (many diffs) |
35 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005823.2 | 84.8% | 5.7% | (many diffs) |
36 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366221.1 | 84% | 7.7% | (many diffs) |
37 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001366222.1 | 83.3% | 7.8% | (many diffs) |
38 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001204387.1 | 82.9% | 5.8% | (many diffs) |
39 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NM_001204388.1 | 82.1% | 5.9% | (many diffs) |
40 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_011512509.2 | 61.6% | 37.1% | (many diffs) |
41 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XR_924108.3 | 59.2% | (many diffs) | |
42 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XM_017005824.2 | 57% | 4.1% | (many diffs) |
43 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XR_001740043.2 | 50.3% | (many diffs) | |
44 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NR_158764.1 | 50.1% | (many diffs) | |
45 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XR_001740036.2 | 49.6% | (many diffs) | |
46 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XR_001740037.2 | 49.4% | (many diffs) | |
47 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XR_001740039.2 | 49.4% | (many diffs) | |
48 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XR_001740044.2 | 49.4% | (many diffs) | |
49 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XR_001740038.2 | 49.3% | (many diffs) | |
50 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NR_158765.1 | 48.9% | (many diffs) | |
51 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NR_158766.1 | 48.7% | (many diffs) | |
52 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | NR_158767.1 | 48.6% | (many diffs) | |
53 | human | 1739 | DLG1 | discs large MAGUK scaffold ... | XR_001740040.2 | 48.1% | (many diffs) | |
54 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | NM_001252433.1 | 88.8% | 21.1% | (many diffs) |
55 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521767.3 | 88.3% | 20.8% | (many diffs) |
56 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_017316866.1 | 87% | 21.5% | (many diffs) |
57 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_017316872.1 | 86.8% | 21.2% | (many diffs) |
58 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | NM_001252434.1 | 86.4% | 21.6% | (many diffs) |
59 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | NM_007862.3 | 85.8% | 20.3% | (many diffs) |
60 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_017316865.1 | 85.7% | 20.4% | (many diffs) |
61 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521763.3 | 85.2% | 20.2% | (many diffs) |
62 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521761.3 | 85.2% | 20.1% | (many diffs) |
63 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521762.3 | 85.1% | 20.1% | (many diffs) |
64 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521765.3 | 84.1% | 20.6% | (many diffs) |
65 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521766.3 | 83.9% | 20.7% | (many diffs) |
66 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521764.3 | 83.7% | 20.4% | (many diffs) |
67 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | NM_001252435.1 | 83.3% | 20.8% | (many diffs) |
68 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521768.3 | 83.3% | 20.8% | (many diffs) |
69 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521769.2 | 78.1% | 7.5% | (many diffs) |
70 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_017316869.1 | 77.3% | 10.6% | (many diffs) |
71 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_017316870.1 | 77.2% | 10.6% | (many diffs) |
72 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | NM_001252436.1 | 76.9% | 7.7% | (many diffs) |
73 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521771.2 | 76.1% | 5.7% | (many diffs) |
74 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521770.3 | 75.8% | 10% | (many diffs) |
75 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_017316867.1 | 75.5% | 7.8% | (many diffs) |
76 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521772.2 | 74.9% | 5.8% | (many diffs) |
77 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_017316868.1 | 74.7% | 5.9% | (many diffs) |
78 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_006521773.2 | 74.1% | 5.9% | (many diffs) |
79 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XM_017316871.1 | 73.2% | 6% | (many diffs) |
80 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XR_384523.3 | 59.2% | (many diffs) | |
81 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XR_001781789.1 | 56.9% | (many diffs) | |
82 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XR_001781790.1 | 56.7% | (many diffs) | |
83 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | XR_001781788.1 | 48.8% | (many diffs) | |
84 | mouse | 13383 | Dlg1 | discs, large homolog 1 (Dro... | NR_045516.1 | 48.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 714
- ORF length:
- 645
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gccggtccgg aagcaagata cccagagagc attgcacctt ttggaggaat 121 atcgttcaaa actaagccaa actgaagaca gacagctcag aagttccata gaacgggtta 181 ttaacatatt tcagagcaac ctctttcagg ctttaataga tattcaagaa ttttatgaag 241 tgaccttact ggataatcca aaatgtatag atcgttcaaa gccgtctgaa ccaattcaac 301 ctgtgaatac ttgggagatt tccagccttc caagctctac tgtgacttca gagacactgc 361 caagcagcct tagccctagt gtagagaaat acaggtatca ggatgaagat acacctcctc 421 aagagcatat ttccccacaa atcacaaatg aagtgatagg tccagaattg gttcatgtct 481 cagagaagaa cttatcagag attgagaatg tccatggatt tgtttctcat tctcatattt 541 caccaataaa ggcaaatcct cccccagtac tggtcaacac agatagcttg aaaacaccaa 601 cttacgttaa tggcacagat gcagattatg aatatgaaga aatcacactt gaaaggggaa 661 attcagggct tggtttcagc attgcagagt acggacaacc cacacattgg agatgactca 721 agtattttca ttaccaaaat tatcacaggg gagcagccgc ccaagatggg aagattgcgg 781 gtcaatgact gtatattaca agtaaatgaa gtagatgttc gtgatgtaac acatagcaaa 841 gcagttgaag cgttgaaaga agcagggtct attgtacgct tgtatgtaaa aagaaggaaa 901 ccagtgtcag aaaaaataat ggaaataaag ctcattaaag gtcctaaagg tcttgggttt 961 agcattgctg gaggtgttgg aaatcagcat attcctgggg ataatagcat ctatgtaacc 1021 aaaataattg aaggaggtgc agcacataag gatggcaaac ttcagattgg agataaactt 1081 ttagcagtga ataacgtatg tttagaagaa gttactcatg aagaagcagt aactgcctta 1141 aagaacacat ctgattttgt ttatttgaaa gtggcaaaac ccacaagtat gtatatgaat 1201 gatggctatg caccacctga tatcaccaac tcttcttctc agcctgttga taaccatgtt 1261 agcccatctt ccttcttggg ccagacacca gcatctccag ccagatactc cccagtttct 1321 aaagcagtac ttggagatga tgaaattaca agggaaccta gaaaagttgt tcttcatcgt 1381 ggctcaacgg gccttggttt caacattgta ggaggagaag atggagaagg aatatttatt 1441 tcctttatct tagccggagg acctgctgat ctaagtggag agctcagaaa aggagatcgt 1501 attatatcgg taaacagtgt tgacctcaga gctgctagtc atgagcaggc agcagctgca 1561 ttgaaaaatg ctggccaggc tgtcacaatt gttgcacaat atcgacctga agaatacagt 1621 cgttttgaag ctaaaataca tgatttacgg gagcagatga tgaatagtag tattagttca 1681 gggtcaggtt ctcttcgaac tagccagaag cgatccctct atgtcagagc cctttttgat 1741 tatgacaaga ctaaagacag tgggcttccc agtcagggac tgaacttcaa atttggagat 1801 atcctccatg ttattaatgc ttctgatgat gaatggtggc aagccaggca ggttacacca 1861 gatggtgaga gcgatgaggt cggagtgatt cccagtaaac gcagagttga gaagaaagaa 1921 cgagcccgat taaaaacagt gaaattcaat tctaaaacga gagataaagg gcagtcattc 1981 aatgacaagc gtaaaaagaa cctcttttcc cgaaaattcc ccttctacaa gaacaaggac 2041 cagagtgagc aggaaacaag tgatgctcac aagtatgtat atgaatgatg gctatgcacc 2101 acctgatatc accaactgtg gtcaagaaga atacgtctta tcttatgaac cagtgaatca 2161 acaagaagtt aattatactc gaccagtgat catattggga cctatgaaag acaggataaa 2221 tgatgacttg atctcagaat ttcctgacaa atttggatcc tgtgttcctc atacaactag 2281 accaaaacga gattatgagg tagatggaag agattatcat tttgtgactt caagagagca 2341 gatggaaaaa gatatccagg aacataaatt cattgaagct ggccagtata aCAATCATCT 2401 ATATGGAACA AGTGTTCAGT CTGTACGAGA AGTAGCAGAA AAGGGCAAAC ACTGTATCCT 2461 TGATGTGTCT GGAAATGCCA TAAAGAGATT ACAGATTGCA CAGCTTTACC CTATCTCCAT 2521 TTTTATTAAA CCCAAATCCA TGGAAAATAT CATGGAAATG AATAAGCGTC TAACAGAAGA 2581 ACAAGCCAGA AAAACATTTG AGAGAGCCAT GAAACTGGAA CAGGAGTTTA CTGAACATTT 2641 CACAGCTATT GTACAGGGGG ATACGCTGGA AGACATTTAC AACCAAGTGA AACAGATCAT 2701 AGAAGAACAA TCTGGTTCTT ACATCTGGGT TCCGGCAAAA GAAAAGCTAT TGCCAACTTT 2761 CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 2821 TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 2881 TTGTGGAAAG GACGATTCGA AGCCCGGCTA AACTAAATCA CGCGTTAAGT Cgacaatcaa 2941 cctctggatt acaaaatttg tgaaagatt