Construct: ORF TRCN0000471116

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018941.3_s317c1
Derived from:
ccsbBroadEn_14615
DNA Barcode:
TTCGAAGCCCGGCTAAACTAAATC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
DLG1 (1739)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000471116
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1739 DLG1 discs large MAGUK scaffold ... NM_001363865.1 98.4% 23% (many diffs)
2 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366207.1 98.4% 23% (many diffs)
3 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366210.1 98.4% 23% (many diffs)
4 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366215.1 98.4% 23% (many diffs)
5 human 1739 DLG1 discs large MAGUK scaffold ... NM_001204386.1 98.2% 23% (many diffs)
6 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366205.1 98.2% 23% (many diffs)
7 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366213.1 98.2% 23% (many diffs)
8 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366211.1 96.4% 23.3% (many diffs)
9 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366217.1 96.4% 23.3% (many diffs)
10 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366206.1 96.4% 21.1% (many diffs)
11 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005811.2 96.4% 21.1% (many diffs)
12 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005812.1 96.4% 21.1% (many diffs)
13 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005813.1 96.4% 21.1% (many diffs)
14 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366220.1 96.3% 21.1% (many diffs)
15 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366204.1 95.6% 23.6% (many diffs)
16 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366208.1 95.6% 23.6% (many diffs)
17 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366209.1 95.6% 23.6% (many diffs)
18 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366212.1 95.6% 23.6% (many diffs)
19 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005819.1 95.6% 23.6% (many diffs)
20 human 1739 DLG1 discs large MAGUK scaffold ... NM_004087.2 94.9% 22.2% (many diffs)
21 human 1739 DLG1 discs large MAGUK scaffold ... XM_005269289.4 94.9% 22.2% (many diffs)
22 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005800.1 94.9% 22.2% (many diffs)
23 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005801.1 94.9% 22.2% (many diffs)
24 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005802.1 94.9% 22.2% (many diffs)
25 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005803.1 94.9% 22.2% (many diffs)
26 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366214.1 94.8% 22.2% (many diffs)
27 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366203.1 94.5% 21.4% (many diffs)
28 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366216.1 93.7% 21.6% (many diffs)
29 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366219.1 93.7% 21.6% (many diffs)
30 human 1739 DLG1 discs large MAGUK scaffold ... NM_001098424.1 92.3% 22.7% (many diffs)
31 human 1739 DLG1 discs large MAGUK scaffold ... NM_001290983.1 92.3% 22.7% (many diffs)
32 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366218.1 92.3% 22.7% (many diffs)
33 human 1739 DLG1 discs large MAGUK scaffold ... XM_011512502.3 92.3% 22.7% (many diffs)
34 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005821.2 85.9% 7.6% (many diffs)
35 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005823.2 84.8% 5.7% (many diffs)
36 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366221.1 84% 7.7% (many diffs)
37 human 1739 DLG1 discs large MAGUK scaffold ... NM_001366222.1 83.3% 7.8% (many diffs)
38 human 1739 DLG1 discs large MAGUK scaffold ... NM_001204387.1 82.9% 5.8% (many diffs)
39 human 1739 DLG1 discs large MAGUK scaffold ... NM_001204388.1 82.1% 5.9% (many diffs)
40 human 1739 DLG1 discs large MAGUK scaffold ... XM_011512509.2 61.6% 37.1% (many diffs)
41 human 1739 DLG1 discs large MAGUK scaffold ... XR_924108.3 59.2% (many diffs)
42 human 1739 DLG1 discs large MAGUK scaffold ... XM_017005824.2 57% 4.1% (many diffs)
43 human 1739 DLG1 discs large MAGUK scaffold ... XR_001740043.2 50.3% (many diffs)
44 human 1739 DLG1 discs large MAGUK scaffold ... NR_158764.1 50.1% (many diffs)
45 human 1739 DLG1 discs large MAGUK scaffold ... XR_001740036.2 49.6% (many diffs)
46 human 1739 DLG1 discs large MAGUK scaffold ... XR_001740037.2 49.4% (many diffs)
47 human 1739 DLG1 discs large MAGUK scaffold ... XR_001740039.2 49.4% (many diffs)
48 human 1739 DLG1 discs large MAGUK scaffold ... XR_001740044.2 49.4% (many diffs)
49 human 1739 DLG1 discs large MAGUK scaffold ... XR_001740038.2 49.3% (many diffs)
50 human 1739 DLG1 discs large MAGUK scaffold ... NR_158765.1 48.9% (many diffs)
51 human 1739 DLG1 discs large MAGUK scaffold ... NR_158766.1 48.7% (many diffs)
52 human 1739 DLG1 discs large MAGUK scaffold ... NR_158767.1 48.6% (many diffs)
53 human 1739 DLG1 discs large MAGUK scaffold ... XR_001740040.2 48.1% (many diffs)
54 mouse 13383 Dlg1 discs, large homolog 1 (Dro... NM_001252433.1 88.8% 21.1% (many diffs)
55 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521767.3 88.3% 20.8% (many diffs)
56 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_017316866.1 87% 21.5% (many diffs)
57 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_017316872.1 86.8% 21.2% (many diffs)
58 mouse 13383 Dlg1 discs, large homolog 1 (Dro... NM_001252434.1 86.4% 21.6% (many diffs)
59 mouse 13383 Dlg1 discs, large homolog 1 (Dro... NM_007862.3 85.8% 20.3% (many diffs)
60 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_017316865.1 85.7% 20.4% (many diffs)
61 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521763.3 85.2% 20.2% (many diffs)
62 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521761.3 85.2% 20.1% (many diffs)
63 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521762.3 85.1% 20.1% (many diffs)
64 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521765.3 84.1% 20.6% (many diffs)
65 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521766.3 83.9% 20.7% (many diffs)
66 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521764.3 83.7% 20.4% (many diffs)
67 mouse 13383 Dlg1 discs, large homolog 1 (Dro... NM_001252435.1 83.3% 20.8% (many diffs)
68 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521768.3 83.3% 20.8% (many diffs)
69 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521769.2 78.1% 7.5% (many diffs)
70 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_017316869.1 77.3% 10.6% (many diffs)
71 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_017316870.1 77.2% 10.6% (many diffs)
72 mouse 13383 Dlg1 discs, large homolog 1 (Dro... NM_001252436.1 76.9% 7.7% (many diffs)
73 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521771.2 76.1% 5.7% (many diffs)
74 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521770.3 75.8% 10% (many diffs)
75 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_017316867.1 75.5% 7.8% (many diffs)
76 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521772.2 74.9% 5.8% (many diffs)
77 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_017316868.1 74.7% 5.9% (many diffs)
78 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_006521773.2 74.1% 5.9% (many diffs)
79 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XM_017316871.1 73.2% 6% (many diffs)
80 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XR_384523.3 59.2% (many diffs)
81 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XR_001781789.1 56.9% (many diffs)
82 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XR_001781790.1 56.7% (many diffs)
83 mouse 13383 Dlg1 discs, large homolog 1 (Dro... XR_001781788.1 48.8% (many diffs)
84 mouse 13383 Dlg1 discs, large homolog 1 (Dro... NR_045516.1 48.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
714
ORF length:
645
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gccggtccgg aagcaagata cccagagagc attgcacctt ttggaggaat
121atcgttcaaa actaagccaa actgaagaca gacagctcag aagttccata gaacgggtta
181ttaacatatt tcagagcaac ctctttcagg ctttaataga tattcaagaa ttttatgaag
241tgaccttact ggataatcca aaatgtatag atcgttcaaa gccgtctgaa ccaattcaac
301ctgtgaatac ttgggagatt tccagccttc caagctctac tgtgacttca gagacactgc
361caagcagcct tagccctagt gtagagaaat acaggtatca ggatgaagat acacctcctc
421aagagcatat ttccccacaa atcacaaatg aagtgatagg tccagaattg gttcatgtct
481cagagaagaa cttatcagag attgagaatg tccatggatt tgtttctcat tctcatattt
541caccaataaa ggcaaatcct cccccagtac tggtcaacac agatagcttg aaaacaccaa
601cttacgttaa tggcacagat gcagattatg aatatgaaga aatcacactt gaaaggggaa
661attcagggct tggtttcagc attgcagagt acggacaacc cacacattgg agatgactca
721agtattttca ttaccaaaat tatcacaggg gagcagccgc ccaagatggg aagattgcgg
781gtcaatgact gtatattaca agtaaatgaa gtagatgttc gtgatgtaac acatagcaaa
841gcagttgaag cgttgaaaga agcagggtct attgtacgct tgtatgtaaa aagaaggaaa
901ccagtgtcag aaaaaataat ggaaataaag ctcattaaag gtcctaaagg tcttgggttt
961agcattgctg gaggtgttgg aaatcagcat attcctgggg ataatagcat ctatgtaacc
1021aaaataattg aaggaggtgc agcacataag gatggcaaac ttcagattgg agataaactt
1081ttagcagtga ataacgtatg tttagaagaa gttactcatg aagaagcagt aactgcctta
1141aagaacacat ctgattttgt ttatttgaaa gtggcaaaac ccacaagtat gtatatgaat
1201gatggctatg caccacctga tatcaccaac tcttcttctc agcctgttga taaccatgtt
1261agcccatctt ccttcttggg ccagacacca gcatctccag ccagatactc cccagtttct
1321aaagcagtac ttggagatga tgaaattaca agggaaccta gaaaagttgt tcttcatcgt
1381ggctcaacgg gccttggttt caacattgta ggaggagaag atggagaagg aatatttatt
1441tcctttatct tagccggagg acctgctgat ctaagtggag agctcagaaa aggagatcgt
1501attatatcgg taaacagtgt tgacctcaga gctgctagtc atgagcaggc agcagctgca
1561ttgaaaaatg ctggccaggc tgtcacaatt gttgcacaat atcgacctga agaatacagt
1621cgttttgaag ctaaaataca tgatttacgg gagcagatga tgaatagtag tattagttca
1681gggtcaggtt ctcttcgaac tagccagaag cgatccctct atgtcagagc cctttttgat
1741tatgacaaga ctaaagacag tgggcttccc agtcagggac tgaacttcaa atttggagat
1801atcctccatg ttattaatgc ttctgatgat gaatggtggc aagccaggca ggttacacca
1861gatggtgaga gcgatgaggt cggagtgatt cccagtaaac gcagagttga gaagaaagaa
1921cgagcccgat taaaaacagt gaaattcaat tctaaaacga gagataaagg gcagtcattc
1981aatgacaagc gtaaaaagaa cctcttttcc cgaaaattcc ccttctacaa gaacaaggac
2041cagagtgagc aggaaacaag tgatgctcac aagtatgtat atgaatgatg gctatgcacc
2101acctgatatc accaactgtg gtcaagaaga atacgtctta tcttatgaac cagtgaatca
2161acaagaagtt aattatactc gaccagtgat catattggga cctatgaaag acaggataaa
2221tgatgacttg atctcagaat ttcctgacaa atttggatcc tgtgttcctc atacaactag
2281accaaaacga gattatgagg tagatggaag agattatcat tttgtgactt caagagagca
2341gatggaaaaa gatatccagg aacataaatt cattgaagct ggccagtata aCAATCATCT
2401ATATGGAACA AGTGTTCAGT CTGTACGAGA AGTAGCAGAA AAGGGCAAAC ACTGTATCCT
2461TGATGTGTCT GGAAATGCCA TAAAGAGATT ACAGATTGCA CAGCTTTACC CTATCTCCAT
2521TTTTATTAAA CCCAAATCCA TGGAAAATAT CATGGAAATG AATAAGCGTC TAACAGAAGA
2581ACAAGCCAGA AAAACATTTG AGAGAGCCAT GAAACTGGAA CAGGAGTTTA CTGAACATTT
2641CACAGCTATT GTACAGGGGG ATACGCTGGA AGACATTTAC AACCAAGTGA AACAGATCAT
2701AGAAGAACAA TCTGGTTCTT ACATCTGGGT TCCGGCAAAA GAAAAGCTAT TGCCAACTTT
2761CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC
2821TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC
2881TTGTGGAAAG GACGATTCGA AGCCCGGCTA AACTAAATCA CGCGTTAAGT Cgacaatcaa
2941cctctggatt acaaaatttg tgaaagatt

Download FASTA (ORF) (Full)