Construct: ORF TRCN0000472583
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002972.1_s317c1
- Derived from:
- ccsbBroadEn_13302
- DNA Barcode:
- ACAAATATGGCAACAGGTCCCGTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MCMDC2 (157777)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472583
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 157777 | MCMDC2 | minichromosome maintenance ... | NM_001136161.2 | 99.1% | 99.1% | 1_15del |
| 2 | human | 157777 | MCMDC2 | minichromosome maintenance ... | XM_011517469.2 | 93% | 93.1% | 1_15del;1770_1884delinsG |
| 3 | human | 157777 | MCMDC2 | minichromosome maintenance ... | NM_001136160.1 | 92.5% | 92.5% | 1_15del;1770_1896delinsG |
| 4 | human | 157777 | MCMDC2 | minichromosome maintenance ... | XM_011517467.3 | 87.6% | 87.7% | 1_15del;1770_2001delinsG |
| 5 | human | 157777 | MCMDC2 | minichromosome maintenance ... | NM_173518.5 | 85.8% | 85.9% | 1_15del;1770_2043delinsG |
| 6 | human | 157777 | MCMDC2 | minichromosome maintenance ... | XM_006716427.3 | 82.8% | 82.5% | (many diffs) |
| 7 | human | 157777 | MCMDC2 | minichromosome maintenance ... | XM_006716429.2 | 81.8% | 81.9% | 1_15del;1070_1071ins81;1689_1962delinsG |
| 8 | human | 157777 | MCMDC2 | minichromosome maintenance ... | XM_011517468.2 | 79.6% | 79.7% | 1_15del;707_708ins126;1644_1917delinsG |
| 9 | human | 157777 | MCMDC2 | minichromosome maintenance ... | XM_005251174.2 | 77.7% | 76.1% | (many diffs) |
| 10 | human | 157777 | MCMDC2 | minichromosome maintenance ... | XM_006716433.3 | 51.4% | 51.4% | 0_1ins711;1044_1317delinsG |
| 11 | human | 157777 | MCMDC2 | minichromosome maintenance ... | XM_024447078.1 | 51.4% | 51.4% | 0_1ins711;1044_1317delinsG |
| 12 | human | 157777 | MCMDC2 | minichromosome maintenance ... | XM_017013140.1 | 47.1% | 47.2% | 0_1ins711;344_345ins81;963_1248delinsG |
| 13 | mouse | 240697 | Mcmdc2 | minichromosome maintenance ... | NM_177722.3 | 74.7% | 73.8% | (many diffs) |
| 14 | mouse | 240697 | Mcmdc2 | minichromosome maintenance ... | XM_011238376.1 | 74.7% | 73.8% | (many diffs) |
| 15 | mouse | 240697 | Mcmdc2 | minichromosome maintenance ... | XM_011238377.2 | 72.1% | 71% | (many diffs) |
| 16 | mouse | 240697 | Mcmdc2 | minichromosome maintenance ... | XM_017320486.1 | 52.4% | 51.6% | (many diffs) |
| 17 | mouse | 240697 | Mcmdc2 | minichromosome maintenance ... | XR_001784301.1 | 18.1% | (many diffs) | |
| 18 | mouse | 240697 | Mcmdc2 | minichromosome maintenance ... | XR_865162.2 | 16.4% | (many diffs) | |
| 19 | mouse | 240697 | Mcmdc2 | minichromosome maintenance ... | XR_001784300.1 | 16.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1821
- ORF length:
- 1755
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa agaggcggcc ctcatctatc ttgacagaag tggaggcctc caaaagttta 121 tagatgattg caagtactac aatgattcaa aacaaagcta tgctgtctat cgattcaaaa 181 ttttaataaa tccctctgat gttgttgaat tagatgctga gcttggaaat cacattttac 241 accaaccttt aaaagctgct gaagtctttc aatcagtctg ttttattgct gttaagactc 301 tctcattaat tggacaattg cagactgaaa cgcaaattaa tatagtgctg aaattaacac 361 atttacctcc cctgccaagt tatggtcttg atctttgtga gtttccactt gattatacat 421 ctcagagatt ttatatgatg caaggaattg tgattgcaat gacaactata accaagtata 481 cacaaggggc aagatttctt tgttcagatg aagcatgccc tctttcaaaa ggatttcagt 541 atataagagt gcatgtgcct ggtgctacag aatctgcaac gataagaaat gactttttgt 601 gtaatctatg tgcatcttca cttcaagaag acagaaaatt tagagtactt ggtgataaac 661 aaatagttga aataattgcc acaaaggcac ttcgtgcttt tcaaggatat tctaacaacc 721 agccatttag gtttcaatca cttacaattt tcctaagaga tgaatcagtg aataaaatga 781 atataggaaa tgaatataaa attattggaa ttccaacctg tgtaaaaacc tcacaaactg 841 ctgtctgtat agaagcaaat agcataactt tttgtaattc aaaagttcct tcaggaatta 901 gtgacaactt caggtgtctc ctctccttaa cttccagctc atgctggaag tttacagcaa 961 tacttgccaa tatctttgca tcacaaatta cccctcctgg gacttacaat ttgctcaagc 1021 tctgtttgtt gatgagtcta gtacagacaa ctgaccgtaa caaggaactg gaagattgcc 1081 tggatatttt aattataaca agtgatactc tactcataga caggcttctg aattttagca 1141 taaaccttgt cccccgtggt atacgtcatc tagtctctac tgaaattttt cccactctat 1201 ccaggaataa gtatggaact ggagcagtta gcattcaggc tggcagtgct ttgctagcta 1261 aaggtggtat ctgctttata ggagacttgg cttcacacaa aaaagataaa cttgaacagc 1321 ttcaaacagt tctggagagc agaagcatca cagtgtacat cccaggaaag aagtttgggg 1381 aggatattga tcaacagatg acttttccag ttcagtgcag tttttggtct tttGTTGATG 1441 TGGATTCATC TTCAAGGAGA AATGCACAGA AAATCAACAC TCTAATTGGT CAGATGGATT 1501 GCAGTTTGAT TCCAGCTAAC CTTGTGGAGG CATTTGGTTT ATTGATTAAC TGCAACGAGT 1561 CATCTCCCTG CCACCCATTT CTTCCTACTG TGCAACACAC TTTGAACAAA GCCATTAATC 1621 CTGAAGGGCT GTTTTATGCG GCTTCTAGAC AGTTCACAAC TGAAGATTTT GAAAAGCTAT 1681 TGGCTTTTGC AAAGAATTTG AATGTAGAAT TCAGCTTGGA AGCAGAAAGA ATGACCCATG 1741 GCTATTATCT AGCAAGTCGC AGAATCAGAA CAGGCTCTGT ATGTGGATCA AAGCTGTCAG 1801 CATCTGCATT AAAATATCTG TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1861 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1921 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAACAA ATATGGCAAC 1981 AGGTCCCGTA ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt