Construct: ORF TRCN0000472750
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017758.1_s317c1
- Derived from:
- ccsbBroadEn_02358
- DNA Barcode:
- CCACTTCCGTACTCCCCGCTCGCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SSX3 (10214)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000472750
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10214 | SSX3 | SSX family member 3 | NM_021014.4 | 100% | 100% | |
| 2 | human | 727837 | SSX2B | SSX family member 2B | NM_001164417.3 | 95.3% | 90.9% | (many diffs) |
| 3 | human | 6757 | SSX2 | SSX family member 2 | NM_175698.2 | 95.3% | 90.9% | (many diffs) |
| 4 | human | 6758 | SSX5 | SSX family member 5 | NM_175723.1 | 93.2% | 84.5% | (many diffs) |
| 5 | human | 6758 | SSX5 | SSX family member 5 | XM_011543949.2 | 93.2% | 84.5% | (many diffs) |
| 6 | human | 6758 | SSX5 | SSX family member 5 | XM_017029758.2 | 93.2% | 84.5% | (many diffs) |
| 7 | human | 280658 | SSX7 | SSX family member 7 | NM_173358.2 | 92.1% | 85.1% | (many diffs) |
| 8 | human | 548313 | SSX4B | SSX family member 4B | NM_001034832.3 | 91.3% | 80.8% | (many diffs) |
| 9 | human | 6759 | SSX4 | SSX family member 4 | NM_005636.4 | 91.3% | 80.8% | (many diffs) |
| 10 | human | 548313 | SSX4B | SSX family member 4B | XM_017029613.1 | 91.3% | 80.8% | (many diffs) |
| 11 | human | 6759 | SSX4 | SSX family member 4 | XM_017029759.1 | 91.3% | 80.8% | (many diffs) |
| 12 | human | 6756 | SSX1 | SSX family member 1 | NM_001278691.2 | 89.5% | 77.6% | (many diffs) |
| 13 | human | 6756 | SSX1 | SSX family member 1 | NM_005635.4 | 89.5% | 77.6% | (many diffs) |
| 14 | human | 10214 | SSX3 | SSX family member 3 | XM_011543885.2 | 86.2% | 78.8% | (many diffs) |
| 15 | human | 6757 | SSX2 | SSX family member 2 | NM_001278697.1 | 83.8% | 79.9% | (many diffs) |
| 16 | human | 727837 | SSX2B | SSX family member 2B | NM_001278702.2 | 83.8% | 79.9% | (many diffs) |
| 17 | human | 280658 | SSX7 | SSX family member 7 | XM_017029430.1 | 77.7% | 71.7% | (many diffs) |
| 18 | human | 6758 | SSX5 | SSX family member 5 | NM_021015.4 | 76.5% | 69.4% | (many diffs) |
| 19 | human | 727837 | SSX2B | SSX family member 2B | NM_001278701.2 | 70.2% | 64.4% | (many diffs) |
| 20 | human | 6757 | SSX2 | SSX family member 2 | NM_003147.5 | 70.2% | 64.4% | (many diffs) |
| 21 | human | 548313 | SSX4B | SSX family member 4B | NM_001040612.2 | 65.5% | 44.4% | (many diffs) |
| 22 | human | 6759 | SSX4 | SSX family member 4 | NM_175729.1 | 65.5% | 44.4% | (many diffs) |
| 23 | human | 280658 | SSX7 | SSX family member 7 | XM_017029431.1 | 63.4% | 53.1% | (many diffs) |
| 24 | human | 280657 | SSX6P | SSX family member 6, pseudo... | NR_028366.1 | 39.6% | (many diffs) | |
| 25 | human | 280659 | SSX8P | SSX family member 8, pseudo... | NR_027250.2 | 35.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 633
- ORF length:
- 564
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacggagat gacacctttg caaggagacc cacggttggt gctcaaatac 121 cagagaagat acaaaaggcc ttcgatgata ttgccaaata cttctctaag gaagagtggg 181 aaaagatgaa agtctcggag aaaaTCGTCT ATGTGTATAT GAAGAGAAAG TATGAGGCCA 241 TGACTAAACT AGGTTTCAAG GCCATCCTCC CATCTTTCAT GCGTAATAAA CGGGTCACAG 301 ACTTCCAGGG GAATGATTTT GATAATGACC CTAACCGTGG GAATCAGGTT CAACGTCCTC 361 AGATGACTTT CGGCAGGCTC CAGGGAATCT TCCCGAAGAT CATGCCCAAG AAGCCAGCAG 421 AGGAAGGAAA TGTTTCGAAG GAAGTGCCAG AAGCATCTGG CCCACAAAAC GATGGGAAAC 481 AGCTGTGCCC CCCGGGAAAA CCAACTACCT CTGAGAAGAT TAACATGATA TCTGGACCCA 541 AAAGGGGGGA ACATGCCTGG ACCCACAGAC TGCGTGAGAG AAAGCAGCTG GTGATTTATG 601 AAGAGATCAG CGATCCTGAG GAAGATGATG AGTTGCCAAC TTTCTTGTAC AAAGTGGTTG 661 ATATCGGTAA GCCTATCCCT AACCCTCTCC TCGGTCTCGA TTCTACGTAG TAATGAACTA 721 GTCCGTAACT TGAAAGTATT TCGATTTCTT GGCTTTATAT ATCTTGTGGA AAGGACGACC 781 ACTTCCGTAC TCCCCGCTCG CGACGCGTTA AGTCgacaat caacctctgg attacaaaat 841 ttgtgaaaga tt