Construct: ORF TRCN0000473724
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010523.1_s317c1
- Derived from:
- ccsbBroadEn_11198
- DNA Barcode:
- CAACCCTTACTTATGAATACCCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TPM3 (7170)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473724
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278191.2 | 87% | 87.3% | (many diffs) |
| 2 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278188.2 | 70.1% | 67.5% | (many diffs) |
| 3 | human | 7170 | TPM3 | tropomyosin 3 | NM_001349679.2 | 63.7% | 63.7% | 1_270del |
| 4 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278189.2 | 59.1% | 57.6% | (many diffs) |
| 5 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364683.1 | 59.1% | 57.6% | (many diffs) |
| 6 | human | 7170 | TPM3 | tropomyosin 3 | NM_001043353.2 | 57.1% | 56% | (many diffs) |
| 7 | human | 7170 | TPM3 | tropomyosin 3 | NM_001043351.2 | 56.6% | 55.6% | (many diffs) |
| 8 | human | 7170 | TPM3 | tropomyosin 3 | NM_152263.4 | 55.4% | 55.4% | 1_381del |
| 9 | human | 7170 | TPM3 | tropomyosin 3 | NM_001043352.2 | 52.7% | 50% | (many diffs) |
| 10 | human | 7170 | TPM3 | tropomyosin 3 | NM_153649.4 | 52.3% | 49.5% | (many diffs) |
| 11 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364682.1 | 51.5% | 50.1% | (many diffs) |
| 12 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364681.2 | 49.8% | 48.7% | (many diffs) |
| 13 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364679.2 | 49.5% | 48.4% | (many diffs) |
| 14 | human | 7170 | TPM3 | tropomyosin 3 | NM_001364680.2 | 45.7% | 43.1% | (many diffs) |
| 15 | human | 7170 | TPM3 | tropomyosin 3 | NM_001278190.1 | 44.7% | 41.1% | (many diffs) |
| 16 | human | 147804 | TPM3P9 | tropomyosin 3 pseudogene 9 | NR_003148.3 | 13.2% | (many diffs) | |
| 17 | human | 7170 | TPM3 | tropomyosin 3 | NR_103461.2 | 12.7% | (many diffs) | |
| 18 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501841.3 | 66.6% | 67.5% | (many diffs) |
| 19 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_017319678.1 | 60.8% | 63.3% | (many diffs) |
| 20 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501838.1 | 60.7% | 62.9% | (many diffs) |
| 21 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501836.2 | 59.4% | 61.8% | (many diffs) |
| 22 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001271764.1 | 56.1% | 57.2% | (many diffs) |
| 23 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_017319677.1 | 56.1% | 57.2% | (many diffs) |
| 24 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XM_006501835.2 | 54.8% | 55.9% | (many diffs) |
| 25 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001293749.1 | 54.4% | 55.7% | (many diffs) |
| 26 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001253740.1 | 54.2% | 55.6% | (many diffs) |
| 27 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001293748.1 | 52.9% | 55% | (many diffs) |
| 28 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164254.1 | 51.6% | 58% | (many diffs) |
| 29 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_001253738.1 | 49.7% | 49.5% | (many diffs) |
| 30 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | NM_001164248.1 | 45.1% | 50.7% | (many diffs) |
| 31 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511042.3 | 45.1% | 50.7% | (many diffs) |
| 32 | mouse | 22003 | Tpm1 | tropomyosin 1, alpha | XM_006511041.1 | 45% | 50.3% | (many diffs) |
| 33 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | NM_022314.3 | 43.5% | 43.1% | (many diffs) |
| 34 | mouse | 59069 | Tpm3 | tropomyosin 3, gamma | XR_001783732.1 | 13.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 540
- ORF length:
- 474
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggttattgaa aaccgggcct taaaagatga agaaaagatg gaactccagg 121 aaatcCAACT CAAAGAAGCT AAGCACATTG CAGAAGAGGC AGATAGGAAG TATGAAGAGG 181 TGGCTCGTAA GTTGGTGATC ATTGAAGGAG ACTTGGAACG CACAGAGGAA CGAGCTGAGC 241 TGGCAGAGTC TAAGTGTTCT GAGCTGGAGG AGGAGCTGAA GAATGTCACC AACAACCTCA 301 AGTCTCTTGA GGCTCAGGCG GAGAAGTACT CTCAAAAAGA AGATAAATAT GAGGAAGAAA 361 TCAAGATTCT TACTGATAAA CTCAAGGAGG CAGAGACCCG TGCTGAGTTT GCTGAGAGAT 421 CGGTAGCCAA GCTGGAAAAG ACAATTGATG ACCTGGAAGA TGAGCTCTAT GCCCAGAAAC 481 TGAAGTACAA GGCCATTAGC GAGGAGCTGG ACCACGCCCT CAATGACATG ACCTCTATAT 541 ACCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 601 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 661 TTTATATATC TTGTGGAAAG GACGACAACC CTTACTTATG AATACCCCCA CGCGTTAAGT 721 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt