Construct: ORF TRCN0000473926
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017582.1_s317c1
- Derived from:
- ccsbBroadEn_07529
- DNA Barcode:
- TGTAACGCGCAGGATGTGGCCCAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AKT3 (10000)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000473926
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_001206729.1 | 99.9% | 99.7% | 836T>G |
| 2 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_181690.2 | 99.9% | 99.7% | 836T>G |
| 3 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_001370074.1 | 95.7% | 94.1% | (many diffs) |
| 4 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | NM_005465.7 | 95.7% | 94.1% | (many diffs) |
| 5 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024446000.1 | 92% | 91.3% | (many diffs) |
| 6 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_016999985.1 | 83% | 81.4% | (many diffs) |
| 7 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024446892.1 | 62% | 57% | (many diffs) |
| 8 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_024447938.1 | 52% | 50.7% | (many diffs) |
| 9 | human | 10000 | AKT3 | AKT serine/threonine kinase 3 | XM_011544014.2 | 46% | 36.5% | (many diffs) |
| 10 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | NM_011785.3 | 89.9% | 94.5% | (many diffs) |
| 11 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496818.3 | 89.9% | 94.5% | (many diffs) |
| 12 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496819.3 | 89.9% | 94.5% | (many diffs) |
| 13 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_011238805.2 | 87.9% | 88.6% | (many diffs) |
| 14 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_017320418.1 | 77.6% | 81.9% | (many diffs) |
| 15 | mouse | 23797 | Akt3 | thymoma viral proto-oncogene 3 | XM_006496821.3 | 50.2% | 48.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1464
- ORF length:
- 1395
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcgatgtt accattgtga aagaaggttg ggttcagaag aggggagaat 121 atataaaaaa ctggaggcca agatacttcc ttttgaagac agatggctca ttcataggat 181 ataaagagaa acctcaagat gtggatttac cttatcccct caacaacttt tcagtggcaa 241 aatgccagtt aatgaaaaca gaacgaccaa agccaaacac atttataatc agatgtctcc 301 agtggactac tgttatagag agaacatttc atgtagatac tccagaggaa agggaagaat 361 ggacagaagc tatccaggct gtagcagaca gactgcagag gcaagaagag gagagaatga 421 attgtagtcc aacttcacaa attgataata taggagagga agagatggat gcctctacaa 481 cccatcataa aagaaagaca atgaatgatt ttgactattt gaaactacta ggtaaaggca 541 cttttgggaa agttattttg gttcgagaga aggcaagtgg aaaatactat gctatgaaga 601 ttctgaagaa agaagtcatt attgcaaagg atgaagtggc acacactcta actgaaagca 661 gagtattaaa gaacactaga catccctttt taacatcctt gaaatattcc ttccagacaa 721 aagaccgttt gtgttttgtg atggaatatg ttaatggggg cgagctgttt ttccatttgt 781 cgagagagcg ggtgttctct gaggaccgca cacgtttcta tggtgcagaa attgtctctg 841 ccttggacta tctacattcc ggaaagattg tgtaccgtga tctcaagttg gagaatctaa 901 tgcgggacaa agatggccac ataaaaatta cagattttgg actttgcaaa gaagggatca 961 cagatgcagc caccatgaag aCATTCTGTG GCACTCCAGA ATATCTGGCA CCAGAGGTGT 1021 TAGAAGATAA TGACTATGGC CGAGCAGTAG ACTGGTGGGG CCTAGGGGTT GTCATGTATG 1081 AAATGATGTG TGGGAGGTTA CCTTTCTACA ACCAGGACCA TGAGAAACTT TTTGAATTAA 1141 TATTAATGGA AGACATTAAA TTTCCTCGAA CACTCTCTTC AGATGCAAAA TCATTGCTTT 1201 CAGGGCTCTT GATAAAGGAT CCAAATAAAC GCCTTGGTGG AGGACCAGAT GATGCAAAAG 1261 AAATTATGAG ACACAGTTTC TTCTCTGGAG TAAACTGGCA AGATGTATAT GATAAAAAGC 1321 TTGTACCTCC TTTTAAACCT CAAGTAACAT CTGAGACAGA TACTAGATAT TTTGATGAAG 1381 AATTTACAGC TCAGACTATT ACAATAACAC CACCTGAAAA ATGTCAGCAA TCAGATTGTG 1441 GCATGCTGGG TAACTGGAAA AAATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1501 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1561 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GTAACGCGCA 1621 GGATGTGGCC CAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1681 att