Construct: ORF TRCN0000473926

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF017582.1_s317c1
Derived from:
ccsbBroadEn_07529
DNA Barcode:
TGTAACGCGCAGGATGTGGCCCAG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AKT3 (10000)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000473926
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10000 AKT3 AKT serine/threonine kinase 3 NM_001206729.1 99.9% 99.7% 836T>G
2 human 10000 AKT3 AKT serine/threonine kinase 3 NM_181690.2 99.9% 99.7% 836T>G
3 human 10000 AKT3 AKT serine/threonine kinase 3 NM_001370074.1 95.7% 94.1% (many diffs)
4 human 10000 AKT3 AKT serine/threonine kinase 3 NM_005465.7 95.7% 94.1% (many diffs)
5 human 10000 AKT3 AKT serine/threonine kinase 3 XM_024446000.1 92% 91.3% (many diffs)
6 human 10000 AKT3 AKT serine/threonine kinase 3 XM_016999985.1 83% 81.4% (many diffs)
7 human 10000 AKT3 AKT serine/threonine kinase 3 XM_024446892.1 62% 57% (many diffs)
8 human 10000 AKT3 AKT serine/threonine kinase 3 XM_024447938.1 52% 50.7% (many diffs)
9 human 10000 AKT3 AKT serine/threonine kinase 3 XM_011544014.2 46% 36.5% (many diffs)
10 mouse 23797 Akt3 thymoma viral proto-oncogene 3 NM_011785.3 89.9% 94.5% (many diffs)
11 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_006496818.3 89.9% 94.5% (many diffs)
12 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_006496819.3 89.9% 94.5% (many diffs)
13 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_011238805.2 87.9% 88.6% (many diffs)
14 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_017320418.1 77.6% 81.9% (many diffs)
15 mouse 23797 Akt3 thymoma viral proto-oncogene 3 XM_006496821.3 50.2% 48.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1464
ORF length:
1395
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gagcgatgtt accattgtga aagaaggttg ggttcagaag aggggagaat
121atataaaaaa ctggaggcca agatacttcc ttttgaagac agatggctca ttcataggat
181ataaagagaa acctcaagat gtggatttac cttatcccct caacaacttt tcagtggcaa
241aatgccagtt aatgaaaaca gaacgaccaa agccaaacac atttataatc agatgtctcc
301agtggactac tgttatagag agaacatttc atgtagatac tccagaggaa agggaagaat
361ggacagaagc tatccaggct gtagcagaca gactgcagag gcaagaagag gagagaatga
421attgtagtcc aacttcacaa attgataata taggagagga agagatggat gcctctacaa
481cccatcataa aagaaagaca atgaatgatt ttgactattt gaaactacta ggtaaaggca
541cttttgggaa agttattttg gttcgagaga aggcaagtgg aaaatactat gctatgaaga
601ttctgaagaa agaagtcatt attgcaaagg atgaagtggc acacactcta actgaaagca
661gagtattaaa gaacactaga catccctttt taacatcctt gaaatattcc ttccagacaa
721aagaccgttt gtgttttgtg atggaatatg ttaatggggg cgagctgttt ttccatttgt
781cgagagagcg ggtgttctct gaggaccgca cacgtttcta tggtgcagaa attgtctctg
841ccttggacta tctacattcc ggaaagattg tgtaccgtga tctcaagttg gagaatctaa
901tgcgggacaa agatggccac ataaaaatta cagattttgg actttgcaaa gaagggatca
961cagatgcagc caccatgaag aCATTCTGTG GCACTCCAGA ATATCTGGCA CCAGAGGTGT
1021TAGAAGATAA TGACTATGGC CGAGCAGTAG ACTGGTGGGG CCTAGGGGTT GTCATGTATG
1081AAATGATGTG TGGGAGGTTA CCTTTCTACA ACCAGGACCA TGAGAAACTT TTTGAATTAA
1141TATTAATGGA AGACATTAAA TTTCCTCGAA CACTCTCTTC AGATGCAAAA TCATTGCTTT
1201CAGGGCTCTT GATAAAGGAT CCAAATAAAC GCCTTGGTGG AGGACCAGAT GATGCAAAAG
1261AAATTATGAG ACACAGTTTC TTCTCTGGAG TAAACTGGCA AGATGTATAT GATAAAAAGC
1321TTGTACCTCC TTTTAAACCT CAAGTAACAT CTGAGACAGA TACTAGATAT TTTGATGAAG
1381AATTTACAGC TCAGACTATT ACAATAACAC CACCTGAAAA ATGTCAGCAA TCAGATTGTG
1441GCATGCTGGG TAACTGGAAA AAATTGCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1501AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1561TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GTAACGCGCA
1621GGATGTGGCC CAGACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1681att

Download FASTA (ORF) (Full)

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