Construct: ORF TRCN0000474279
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007518.4_s317c1
- Derived from:
- ccsbBroadEn_06450
- DNA Barcode:
- GTTTGCGACGCCATTAAGAACTAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ILK (3611)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000474279
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3611 | ILK | integrin linked kinase | NM_001014794.3 | 99.8% | 100% | 297C>T;819G>A |
| 2 | human | 3611 | ILK | integrin linked kinase | NM_001014795.3 | 99.8% | 100% | 297C>T;819G>A |
| 3 | human | 3611 | ILK | integrin linked kinase | NM_004517.4 | 99.8% | 100% | 297C>T;819G>A |
| 4 | human | 3611 | ILK | integrin linked kinase | XM_005252904.5 | 99.8% | 100% | 297C>T;819G>A |
| 5 | human | 3611 | ILK | integrin linked kinase | XM_011520065.1 | 99.8% | 100% | 297C>T;819G>A |
| 6 | human | 3611 | ILK | integrin linked kinase | XM_024448494.1 | 93.4% | 93.5% | 297C>T;447_539del;912G>A |
| 7 | human | 3611 | ILK | integrin linked kinase | XM_024448495.1 | 93.4% | 93.5% | 297C>T;447_539del;912G>A |
| 8 | human | 3611 | ILK | integrin linked kinase | XM_024448496.1 | 93.4% | 93.5% | 297C>T;447_539del;912G>A |
| 9 | human | 3611 | ILK | integrin linked kinase | XM_024448497.1 | 93.4% | 93.5% | 297C>T;447_539del;912G>A |
| 10 | human | 3611 | ILK | integrin linked kinase | XM_017017672.1 | 88.5% | 88.7% | 0_1ins153;144C>T;666G>A |
| 11 | human | 3611 | ILK | integrin linked kinase | XM_024448498.1 | 88.5% | 88.7% | 0_1ins153;144C>T;666G>A |
| 12 | human | 3611 | ILK | integrin linked kinase | XM_024448499.1 | 88.5% | 88.7% | 0_1ins153;144C>T;666G>A |
| 13 | human | 3611 | ILK | integrin linked kinase | NM_001278441.2 | 86.3% | 82.7% | (many diffs) |
| 14 | human | 3611 | ILK | integrin linked kinase | NM_001278442.1 | 70.2% | 70.3% | 0_1ins402;417G>A |
| 15 | human | 3611 | ILK | integrin linked kinase | XM_005252905.3 | 70.2% | 70.3% | 0_1ins402;417G>A |
| 16 | human | 3611 | ILK | integrin linked kinase | XM_024448500.1 | 65.7% | 65.8% | 0_1ins402;45_137del;510G>A |
| 17 | mouse | 16202 | Ilk | integrin linked kinase | NM_001161724.1 | 91.8% | 99.5% | (many diffs) |
| 18 | mouse | 16202 | Ilk | integrin linked kinase | NM_010562.2 | 91.8% | 99.5% | (many diffs) |
| 19 | mouse | 16202 | Ilk | integrin linked kinase | XM_006507389.3 | 85.7% | 92.9% | (many diffs) |
| 20 | mouse | 16202 | Ilk | integrin linked kinase | XM_006507390.3 | 85.7% | 92.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1422
- ORF length:
- 1356
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga cgacattttc actcagtgcc gggagggcaa cgcagtcgcc gttcgcctgt 121 ggctggacaa cacggagaac gacctcaacc agggggacga tcatggcttc tcccccttgc 181 actgggcctg ccgagagggc cgctctgctg tggttgagat gttgatcatg cggggggcac 241 ggatcaatgt aatgaaccgt ggggatgaca cccccctgca tctggcagcc agtcatggac 301 accgtgatat tgtacagaag ctattgcagt acaaggcaga catcaatgca gtgaatgaac 361 atgggaatgt gcccctgcac tatgcctgtt tttggggcca agatcaagtg gcagaggacc 421 tggtggcaaa tggggccctt gtcagcatct gtaacaagta tggagagatg cctgtggaca 481 aagccaaggc acccctgaga gagcttctcc gagagcgggc agagaagatg ggccagaatc 541 tcaaccgtat tccatacaag gacacattct ggaaggggac cacccgcact cggccccgaa 601 atggaaccct gaacaaacac tctggcattg acttcaaaca gcttaacttc ctgacgaagc 661 tcaacgagaa tcactctgga gagctatgga agggccgctg gcagggcaat gacattgtcg 721 tgaaggtgct gaaggttcga gactggagta caaggaagag cagggacttc aatgaagagt 781 gtccccggct caggattttc tcgcatccaa atgtgctccc agtgctaggt gcctgccagt 841 ctccacctgc tcctcatcct actctcatca cacactggat gccatatgga tccctcTACA 901 ATGTACTACA TGAAGGCACC AATTTCGTCG TGGACCAGAG CCAGGCTGTG AAGTTTGCTT 961 TGGACATGGC AAGGGGCATG GCCTTCCTAC ACACACTAGA GCCCCTCATC CCACGACATG 1021 CACTCAATAG CCGTAGTGTA ATGATTGATG AGGACATGAC TGCCCGAATT AGCATGGCTG 1081 ATGTCAAGTT CTCTTTCCAA TGTCCTGGTC GCATGTATGC ACCTGCCTGG GTAGCCCCCG 1141 AAGCTCTGCA GAAGAAGCCT GAAGACACAA ACAGACGCTC AGCAGACATG TGGAGTTTTG 1201 CAGTGCTTCT GTGGGAACTG GTGACACGGG AGGTACCCTT TGCTGACCTC TCCAATATGG 1261 AGATTGGAAT GAAGGTGGCA TTGGAAGGCC TTCGGCCTAC CATCCCACCA GGTATTTCCC 1321 CTCATGTGTG TAAGCTCATG AAGATCTGCA TGAATGAAGA CCCTGCAAAG CGACCCAAAT 1381 TTGACATGAT TGTGCCTATC CTTGAGAAGA TGCAGGACAA GTACCCAACT TTCTTGTACA 1441 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 1501 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 1561 AGGACGAGTT TGCGACGCCA TTAAGAACTA AACGCGTTAA GTCgacaatc aacctctgga 1621 ttacaaaatt tgtgaaagat t