Construct: ORF TRCN0000475160

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011288.1_s317c1
Derived from:
ccsbBroadEn_08344
DNA Barcode:
TTTCTATAGAAACAAGTAGGGTAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM8B (51754)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000475160
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 51754 TMEM8B transmembrane protein 8B NM_001363621.1 99.9% 99.7% 532C>T
2 human 51754 TMEM8B transmembrane protein 8B NM_016446.3 99.9% 99.7% 532C>T
3 human 51754 TMEM8B transmembrane protein 8B XM_011517905.1 94.4% 31.3% 279_300del;554C>T;1002_1003ins34
4 human 51754 TMEM8B transmembrane protein 8B NM_001363622.1 84.3% 77.4% (many diffs)
5 human 51754 TMEM8B transmembrane protein 8B XM_024447570.1 84.3% 77.4% (many diffs)
6 human 51754 TMEM8B transmembrane protein 8B XM_011517903.2 78.8% 72.4% (many diffs)
7 human 51754 TMEM8B transmembrane protein 8B NM_001363625.1 70.1% 14.3% (many diffs)
8 human 51754 TMEM8B transmembrane protein 8B NM_001042589.2 67.5% 61.3% (many diffs)
9 human 51754 TMEM8B transmembrane protein 8B XM_005251483.4 66% 60% (many diffs)
10 human 51754 TMEM8B transmembrane protein 8B XM_011517915.2 66% 60% (many diffs)
11 human 51754 TMEM8B transmembrane protein 8B XM_017014806.1 66% 60% (many diffs)
12 human 51754 TMEM8B transmembrane protein 8B XM_024447571.1 66% 60% (many diffs)
13 human 51754 TMEM8B transmembrane protein 8B XM_011517902.2 64% 58.1% (many diffs)
14 human 51754 TMEM8B transmembrane protein 8B XM_011517901.2 62.7% 57% (many diffs)
15 human 51754 TMEM8B transmembrane protein 8B XM_011517908.2 62.7% 57% (many diffs)
16 human 51754 TMEM8B transmembrane protein 8B XM_011517910.3 62.7% 57% (many diffs)
17 human 51754 TMEM8B transmembrane protein 8B XM_011517911.2 62.7% 57% (many diffs)
18 human 51754 TMEM8B transmembrane protein 8B XM_011517912.2 62.7% 57% (many diffs)
19 human 51754 TMEM8B transmembrane protein 8B XM_011517913.2 62.7% 57% (many diffs)
20 human 51754 TMEM8B transmembrane protein 8B XM_011517914.2 62.7% 57% (many diffs)
21 human 51754 TMEM8B transmembrane protein 8B XM_011517916.2 54.8% 43.3% (many diffs)
22 human 51754 TMEM8B transmembrane protein 8B XM_017014807.1 48.7% 40.6% (many diffs)
23 human 51754 TMEM8B transmembrane protein 8B XM_011517918.3 47.6% 41.9% (many diffs)
24 human 51754 TMEM8B transmembrane protein 8B XM_017014805.1 47.2% 43% (many diffs)
25 human 51754 TMEM8B transmembrane protein 8B NM_001363620.1 42.7% 42.6% 1_1356del;1888C>T
26 human 51754 TMEM8B transmembrane protein 8B NM_001042590.4 34.6% 31.4% (many diffs)
27 human 51754 TMEM8B transmembrane protein 8B XR_002956793.1 20.6% (many diffs)
28 human 51754 TMEM8B transmembrane protein 8B XR_001746322.1 18.8% (many diffs)
29 mouse 242409 Tmem8b transmembrane protein 8B NM_001085508.2 58% 58.8% (many diffs)
30 mouse 242409 Tmem8b transmembrane protein 8B XM_011250030.2 58% 58.8% (many diffs)
31 mouse 242409 Tmem8b transmembrane protein 8B XM_006537910.2 53.6% 54.2% (many diffs)
32 mouse 242409 Tmem8b transmembrane protein 8B XM_011250027.2 29.8% 29.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1083
ORF length:
1014
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccat gaacatgccc cagtccctgg gcaaccagcc actgccccca gaaccgccat
121cccttggaac ccctgcggag gggcctggga ccacgtcccc acccgagcac tgctggccag
181tgcgcccgac tctgcgcaac gagctggaca ccttctctgt ccacttctac atcttctttg
241gcccaagtgt ggcccttccc cctgagcgcc cagccgtgtt cgccatgagg ctgttgccag
301tgctggacag tggaggcgtc ctcagcctgg agctccagct caatgcgagc tccgtgcgcc
361aggaaaacgt gacggtgttt ggatgcttga ctcacgaggt gcccttgagc ctgggggatg
421cagcagtgac ctgttccaaa gagtccctgg ccggcttcct cctctctgtc agtgccacca
481ccagggttgc caggctgcga atcccattcc cgcagacggg gacctggttc ctggccctcc
541gctccctgtg cggggtgggg cctcggttcg tgcggtgccg caacgcgacg gccgaggtgt
601ggatgcgcac cttcctgtcc ccatgcgtgg acgactgcgg gccctacggc cagtgcaagc
661tgctgcgcac acacaattat ctgtacgcag cctgcgagtg caaggccggg tggagaggct
721ggggctgcac cgacagtgca gatgcgctca cctatggatt ccagctgctg tccacactcc
781tgctctgcct gagcaacctc atgtttctgc cacctgtggt cctggccatt cggagtcgat
841atgtgctgga agctgcagtc tacaccttca ccatgttcTT CTCCACGGTA TGCGGTGGTG
901TCTGCATCTT ATCACTGGGT GCTTGTGCAT GGTGGTGGGT CACAGTCTGT ATTTCCACCA
961CGTTCTCCGA GGGTTTGGGA ATGTCTGTGC CTTCACTGTG TCTTCTACAG ACAGAGACAG
1021CAGTGCTTCC CAAACTGTCA TGCATAGATA ATGGTCATTT TTGTAAGACA CATTGGAGTA
1081AATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1141TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1201GGCTTTATAT ATCTTGTGGA AAGGACGATT TCTATAGAAA CAAGTAGGGT ATACGCGTTA
1261AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)

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