Construct: ORF TRCN0000475160
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011288.1_s317c1
- Derived from:
- ccsbBroadEn_08344
- DNA Barcode:
- TTTCTATAGAAACAAGTAGGGTAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM8B (51754)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000475160
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51754 | TMEM8B | transmembrane protein 8B | NM_001363621.1 | 99.9% | 99.7% | 532C>T |
| 2 | human | 51754 | TMEM8B | transmembrane protein 8B | NM_016446.3 | 99.9% | 99.7% | 532C>T |
| 3 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517905.1 | 94.4% | 31.3% | 279_300del;554C>T;1002_1003ins34 |
| 4 | human | 51754 | TMEM8B | transmembrane protein 8B | NM_001363622.1 | 84.3% | 77.4% | (many diffs) |
| 5 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_024447570.1 | 84.3% | 77.4% | (many diffs) |
| 6 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517903.2 | 78.8% | 72.4% | (many diffs) |
| 7 | human | 51754 | TMEM8B | transmembrane protein 8B | NM_001363625.1 | 70.1% | 14.3% | (many diffs) |
| 8 | human | 51754 | TMEM8B | transmembrane protein 8B | NM_001042589.2 | 67.5% | 61.3% | (many diffs) |
| 9 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_005251483.4 | 66% | 60% | (many diffs) |
| 10 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517915.2 | 66% | 60% | (many diffs) |
| 11 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_017014806.1 | 66% | 60% | (many diffs) |
| 12 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_024447571.1 | 66% | 60% | (many diffs) |
| 13 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517902.2 | 64% | 58.1% | (many diffs) |
| 14 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517901.2 | 62.7% | 57% | (many diffs) |
| 15 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517908.2 | 62.7% | 57% | (many diffs) |
| 16 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517910.3 | 62.7% | 57% | (many diffs) |
| 17 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517911.2 | 62.7% | 57% | (many diffs) |
| 18 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517912.2 | 62.7% | 57% | (many diffs) |
| 19 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517913.2 | 62.7% | 57% | (many diffs) |
| 20 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517914.2 | 62.7% | 57% | (many diffs) |
| 21 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517916.2 | 54.8% | 43.3% | (many diffs) |
| 22 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_017014807.1 | 48.7% | 40.6% | (many diffs) |
| 23 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_011517918.3 | 47.6% | 41.9% | (many diffs) |
| 24 | human | 51754 | TMEM8B | transmembrane protein 8B | XM_017014805.1 | 47.2% | 43% | (many diffs) |
| 25 | human | 51754 | TMEM8B | transmembrane protein 8B | NM_001363620.1 | 42.7% | 42.6% | 1_1356del;1888C>T |
| 26 | human | 51754 | TMEM8B | transmembrane protein 8B | NM_001042590.4 | 34.6% | 31.4% | (many diffs) |
| 27 | human | 51754 | TMEM8B | transmembrane protein 8B | XR_002956793.1 | 20.6% | (many diffs) | |
| 28 | human | 51754 | TMEM8B | transmembrane protein 8B | XR_001746322.1 | 18.8% | (many diffs) | |
| 29 | mouse | 242409 | Tmem8b | transmembrane protein 8B | NM_001085508.2 | 58% | 58.8% | (many diffs) |
| 30 | mouse | 242409 | Tmem8b | transmembrane protein 8B | XM_011250030.2 | 58% | 58.8% | (many diffs) |
| 31 | mouse | 242409 | Tmem8b | transmembrane protein 8B | XM_006537910.2 | 53.6% | 54.2% | (many diffs) |
| 32 | mouse | 242409 | Tmem8b | transmembrane protein 8B | XM_011250027.2 | 29.8% | 29.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1083
- ORF length:
- 1014
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaacatgccc cagtccctgg gcaaccagcc actgccccca gaaccgccat 121 cccttggaac ccctgcggag gggcctggga ccacgtcccc acccgagcac tgctggccag 181 tgcgcccgac tctgcgcaac gagctggaca ccttctctgt ccacttctac atcttctttg 241 gcccaagtgt ggcccttccc cctgagcgcc cagccgtgtt cgccatgagg ctgttgccag 301 tgctggacag tggaggcgtc ctcagcctgg agctccagct caatgcgagc tccgtgcgcc 361 aggaaaacgt gacggtgttt ggatgcttga ctcacgaggt gcccttgagc ctgggggatg 421 cagcagtgac ctgttccaaa gagtccctgg ccggcttcct cctctctgtc agtgccacca 481 ccagggttgc caggctgcga atcccattcc cgcagacggg gacctggttc ctggccctcc 541 gctccctgtg cggggtgggg cctcggttcg tgcggtgccg caacgcgacg gccgaggtgt 601 ggatgcgcac cttcctgtcc ccatgcgtgg acgactgcgg gccctacggc cagtgcaagc 661 tgctgcgcac acacaattat ctgtacgcag cctgcgagtg caaggccggg tggagaggct 721 ggggctgcac cgacagtgca gatgcgctca cctatggatt ccagctgctg tccacactcc 781 tgctctgcct gagcaacctc atgtttctgc cacctgtggt cctggccatt cggagtcgat 841 atgtgctgga agctgcagtc tacaccttca ccatgttcTT CTCCACGGTA TGCGGTGGTG 901 TCTGCATCTT ATCACTGGGT GCTTGTGCAT GGTGGTGGGT CACAGTCTGT ATTTCCACCA 961 CGTTCTCCGA GGGTTTGGGA ATGTCTGTGC CTTCACTGTG TCTTCTACAG ACAGAGACAG 1021 CAGTGCTTCC CAAACTGTCA TGCATAGATA ATGGTCATTT TTGTAAGACA CATTGGAGTA 1081 AATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1141 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1201 GGCTTTATAT ATCTTGTGGA AAGGACGATT TCTATAGAAA CAAGTAGGGT ATACGCGTTA 1261 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt