Construct: ORF TRCN0000477056
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001214.1_s317c1
- Derived from:
- ccsbBroadEn_07828
- DNA Barcode:
- CCAAAGGCACCTCCGCCCTGATCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOLGA8A (23015)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000477056
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 440270 | GOLGA8B | golgin A8 family member B | NM_001023567.5 | 99.7% | 99.1% | (many diffs) |
2 | human | 23015 | GOLGA8A | golgin A8 family member A | NM_181077.4 | 99.6% | 99% | (many diffs) |
3 | human | 23015 | GOLGA8A | golgin A8 family member A | NM_001368071.2 | 99.5% | 98.8% | (many diffs) |
4 | human | 23015 | GOLGA8A | golgin A8 family member A | NM_001368072.1 | 99.5% | 98.8% | (many diffs) |
5 | human | 283768 | GOLGA8G | golgin A8 family member G | NM_001350919.2 | 80.1% | 69.8% | (many diffs) |
6 | human | 100132565 | GOLGA8F | golgin A8 family member F | NM_001350920.1 | 80.1% | 69.8% | (many diffs) |
7 | human | 100132565 | GOLGA8F | golgin A8 family member F | XM_024449809.1 | 77.9% | 67.6% | (many diffs) |
8 | human | 283768 | GOLGA8G | golgin A8 family member G | XM_024449905.1 | 77.9% | 67.6% | (many diffs) |
9 | human | 100132565 | GOLGA8F | golgin A8 family member F | XM_024449808.1 | 77.8% | 67.5% | (many diffs) |
10 | human | 283768 | GOLGA8G | golgin A8 family member G | XM_024449904.1 | 77.8% | 67.5% | (many diffs) |
11 | human | 653720 | GOLGA8M | golgin A8 family member M | NM_001282468.1 | 77.6% | 65% | (many diffs) |
12 | human | 23015 | GOLGA8A | golgin A8 family member A | XM_017022020.2 | 75.7% | 75.1% | (many diffs) |
13 | human | 100132565 | GOLGA8F | golgin A8 family member F | XR_002957623.1 | 64% | (many diffs) | |
14 | human | 283768 | GOLGA8G | golgin A8 family member G | XR_002957634.1 | 64% | (many diffs) | |
15 | human | 653720 | GOLGA8M | golgin A8 family member M | XM_011521951.3 | 59.5% | 50.6% | (many diffs) |
16 | human | 653720 | GOLGA8M | golgin A8 family member M | XM_011521954.3 | 59.5% | 50.6% | (many diffs) |
17 | human | 653720 | GOLGA8M | golgin A8 family member M | XM_024450037.1 | 59.5% | 50.6% | (many diffs) |
18 | human | 283768 | GOLGA8G | golgin A8 family member G | NM_001368078.1 | 55.4% | 49.2% | (many diffs) |
19 | human | 283768 | GOLGA8G | golgin A8 family member G | NM_001368080.1 | 55.4% | 49% | (many diffs) |
20 | human | 100132565 | GOLGA8F | golgin A8 family member F | XM_024449812.1 | 55.4% | 49% | (many diffs) |
21 | human | 100132565 | GOLGA8F | golgin A8 family member F | XM_024449813.1 | 55.4% | 49% | (many diffs) |
22 | human | 100132565 | GOLGA8F | golgin A8 family member F | XM_024449810.1 | 53.9% | 47.3% | (many diffs) |
23 | human | 100132565 | GOLGA8F | golgin A8 family member F | XM_024449811.1 | 53.9% | 47.3% | (many diffs) |
24 | human | 283768 | GOLGA8G | golgin A8 family member G | XM_024449906.1 | 53.9% | 47.3% | (many diffs) |
25 | human | 283768 | GOLGA8G | golgin A8 family member G | XM_024449907.1 | 53.9% | 47.3% | (many diffs) |
26 | human | 653061 | GOLGA8S | golgin A8 family member S | NM_001355465.1 | 51.8% | 45.1% | (many diffs) |
27 | human | 653061 | GOLGA8S | golgin A8 family member S | XM_024450030.1 | 51.8% | 45.1% | (many diffs) |
28 | human | 100132565 | GOLGA8F | golgin A8 family member F | NR_033351.1 | 34.7% | (many diffs) | |
29 | human | 729786 | GOLGA8CP | golgin A8 family member C, ... | NR_027411.2 | 29.7% | (many diffs) | |
30 | human | 390535 | GOLGA8EP | golgin A8 family member E, ... | NR_033350.1 | 29.7% | (many diffs) | |
31 | human | 100132979 | GOLGA8DP | golgin A8 family member D, ... | NR_027407.1 | 29.3% | (many diffs) | |
32 | human | 440270 | GOLGA8B | golgin A8 family member B | NR_027410.1 | 27.6% | (many diffs) | |
33 | human | 23015 | GOLGA8A | golgin A8 family member A | NR_027409.2 | 26.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1878
- ORF length:
- 1809
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagaagaa actggacaga gtaaattagc tgcagccaag aaaaagttca 121 aagaatattg gcagagaaac cgccctggtg ttccagcagc agcgaagagg aacacgaaag 181 caaatggcag tagccctgag acggccgctt ctggtggttg ccactcatct gaggcttcct 241 cctccgcctc ctcctctctg catgcgcgtc agagcccgtg ccaagaacaa gcagcagtcc 301 tgaactcgag gtccataaaa atcagtcgac tgaatgacac catcaaatct ttgaaacaac 361 agaagaaaca agtggaacat cagctggaag aagaaaagaa agcaaacaat gagaaacaga 421 aagctgaaag ggagctagag ggtcaaatcc agagattgaa cacagagaaa aagaaactaa 481 atacggacct gtatcacatg aaacattctc tcagatactt tgaagaagag tccaaggatc 541 tggccggccg cctgcaacgt tcatcgcagc gtataggaga gttagagtgg tctctctgtg 601 ctgtcgccgc cacacagaag aagaagccgg atgggttctc gagccgcagt aaagcacttc 661 tcaagcggca gttagagcag tccatacggg agcagatact gctgaaagga cacgtgacac 721 agttgaagga gtcgcttaaa gaagtccagc tggagagaga tcaatatgct gaacaaataa 781 aaggagagag ggcccagtgg cagcagagga tgaggaaaat gtcgcaggag gtttgcacat 841 tgaaggagga gaagaagcat gatacgcatc gggtagagga gctggagagg agcttgtcca 901 gactcaaaaa ccagatggct gagccactgc ccccggatgc cccagcagtg tcctctgagg 961 tggagctgca agacctgagg aaggagctgg agagagtggc aggagagctc caggctcagg 1021 tggaaaacaa tcagtgcatc agtctcctga accgtgggca aaaggagagg cttcgcgagc 1081 aggaggagag gcttcaggag cagcaggaga ggcttcggga acaggagaag aggcttcagc 1141 agctggccga gccacagagc gacttggagg agctgaagca cgagaacaag agcgcactgc 1201 agttggagca gcaagtaaag gagctgcagg agaagctggg ccaggtgacg gagacgctca 1261 cctcggccga gaaggagcca gaggcagcag tcccagcctc agggactggg ggcgagtcta 1321 gcggccttat ggacctcctg gaggagaagg tggacctgag ggagcatgtg gagaaactgg 1381 aacttggatt catccagtac cggagagaga gatgccatca gaaagtacat cgccttctaa 1441 cagagccagg ggacagtgcc aaagatgcgt caccgggagg aggccatCAT CAGGCTGGCC 1501 CAGGACGAGG AGGAGAGGAA GGTGAAGCTG TTGGAGCTGC AGGAGATGGT GTTGCGGCTT 1561 GTGGCAGCTA CAGCGAGGGG CACGGCAAAT TCCTGGCCGC TGCCCGGAAC CCTGCTGCTG 1621 AACCCAGTCC AGGAGCCCCA GCCCCCCAGG AGCTCGGGGC TGCCGACAAG CATGGTGATC 1681 TTTGTGAGGC GAGCCTCACC AACAGCGTGG AGCCTGCACA AGGAGAAGCC AGGGAGGGTT 1741 CTTCCCAGGA CAACCCTACT GCACAGCCAG TCCTGCAGCT CCTTGGTGAG ATGCAGGACC 1801 ACCAGGAGCA CCCAGGCTTG GGCAGCAACT GCTGTGTGCC ATGCTTTTGC TGGGCTTGGC 1861 TGCCGAGAAG AAGGAGATTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1921 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1981 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACCAAAGG CACCTCCGCC 2041 CTGATCTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt