Transcript: Human XM_024449811.1

PREDICTED: Homo sapiens golgin A8 family member F (GOLGA8F), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
GOLGA8F (100132565)
Length:
2671
CDS:
901..2256

Additional Resources:

NCBI RefSeq record:
XM_024449811.1
NBCI Gene record:
GOLGA8F (100132565)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024449811.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000152236 CAGTTGAAGGAGTCACTTAAA pLKO.1 961 CDS 100% 13.200 6.600 Y GOLGA8EP n/a
2 TRCN0000151329 GAGATGCCATCAGAAAGTTTA pLKO.1 1806 CDS 100% 13.200 6.600 Y GOLGA8F n/a
3 TRCN0000269218 CAGTTGGAGCAGCAAGTAAAG pLKO_005 1441 CDS 100% 10.800 5.400 Y GOLGA6L9 n/a
4 TRCN0000195845 CCCACAAGCATGGTGATCTTT pLKO.1 2057 CDS 100% 5.625 2.813 Y GOLGA8EP n/a
5 TRCN0000153701 CAAGGAGCTGAACAATGAGAA pLKO.1 1407 CDS 100% 4.950 2.475 Y GOLGA8G n/a
6 TRCN0000158108 CAGAGAGAGATGCCATCAGAA pLKO.1 1800 CDS 100% 4.950 2.475 Y GOLGA8G n/a
7 TRCN0000157310 CAGCGCATAAGTCTCCTGAAT pLKO.1 1273 CDS 100% 4.950 2.475 Y GOLGA8G n/a
8 TRCN0000158334 CTACAGTTGGAGCAGCAAGTA pLKO.1 1438 CDS 100% 4.950 2.475 Y GOLGA8G n/a
9 TRCN0000157512 GCTCGTTGAAGAAGGAGAAGA pLKO.1 1076 CDS 100% 4.950 2.475 Y GOLGA8F n/a
10 TRCN0000153184 GTGGGAGTTCAAACACACAAA pLKO.1 2535 3UTR 100% 4.950 2.475 Y GOLGA8F n/a
11 TRCN0000157882 CTCAAACACCAGATGGCTGAA pLKO.1 1144 CDS 100% 4.050 2.025 Y GOLGA8G n/a
12 TRCN0000152781 GAGAGGGATGAATATGCTGAA pLKO.1 994 CDS 100% 4.050 2.025 Y GOLGA8G n/a
13 TRCN0000153085 GCAACATTCATTGCAGCGTAA pLKO.1 795 5UTR 100% 4.050 2.025 Y GOLGA8IP n/a
14 TRCN0000150773 GCATGATAAATATCGGGTAGA pLKO.1 1098 CDS 100% 4.050 2.025 Y GOLGA8G n/a
15 TRCN0000152577 GTCCAAACTCAAACACCAGAT pLKO.1 1137 CDS 100% 4.050 2.025 Y GOLGA8G n/a
16 TRCN0000153484 CACAGCAAATTCTTGGTGACT pLKO.1 1975 CDS 100% 2.640 1.320 Y GOLGA8F n/a
17 TRCN0000154849 GAACAGCTTCAAGGAGCTGAA pLKO.1 1398 CDS 100% 0.405 0.203 Y GOLGA8EP n/a
18 TRCN0000152586 GACACAGTTGAAGGAGTCATT pLKO.1 957 CDS 100% 4.950 2.475 Y GOLGA8IP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024449811.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10341 pDONR223 100% 95.3% 95.3% None 753_815del n/a
2 ccsbBroad304_10341 pLX_304 0% 95.3% 95.3% V5 753_815del n/a
3 TRCN0000476380 AGTGATAACGTTCAAGGTCGCGGA pLX_317 28.7% 95.3% 95.3% V5 753_815del n/a
4 ccsbBroadEn_16170 pDONR223 0% 94.4% 93.7% None (many diffs) n/a
5 ccsbBroad304_16170 pLX_304 0% 94.4% 93.7% V5 (many diffs) n/a
6 ccsbBroadEn_16172 pDONR223 0% 62.7% 62.7% None 360_863del n/a
7 ccsbBroad304_16172 pLX_304 0% 62.7% 62.7% V5 360_863del n/a
8 TRCN0000480289 TACTAACTTATTTTTTGACAAACC pLX_317 100% 14.2% 14.1% V5 (not translated due to prior stop codon) 194_1353del n/a
9 ccsbBroadEn_07828 pDONR223 100% 53.9% 47.3% None (many diffs) n/a
10 ccsbBroad304_07828 pLX_304 0% 53.9% 47.3% V5 (many diffs) n/a
11 TRCN0000477056 CCAAAGGCACCTCCGCCCTGATCT pLX_317 21.8% 53.9% 47.3% V5 (many diffs) n/a
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