Transcript: Human NR_027407.1

Homo sapiens golgin A8 family member D, pseudogene (GOLGA8DP), non-coding RNA.

Source:
NCBI, updated 2018-06-03
Taxon:
Homo sapiens (human)
Gene:
GOLGA8DP (100132979)
Length:
5339
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_027407.1
NBCI Gene record:
GOLGA8DP (100132979)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_027407.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000183650 CCACTCTTTGTGTAGATATTT pLKO.1 4518 3UTR 100% 15.000 7.500 Y GOLGA8EP n/a
2 TRCN0000152236 CAGTTGAAGGAGTCACTTAAA pLKO.1 1076 3UTR 100% 13.200 6.600 Y GOLGA8EP n/a
3 TRCN0000153758 CCACCAAGAGTACTGACATTT pLKO.1 2820 3UTR 100% 13.200 6.600 Y GOLGA8F n/a
4 TRCN0000269218 CAGTTGGAGCAGCAAGTAAAG pLKO_005 1556 3UTR 100% 10.800 5.400 Y GOLGA6L9 n/a
5 TRCN0000195845 CCCACAAGCATGGTGATCTTT pLKO.1 2109 3UTR 100% 5.625 2.813 Y GOLGA8EP n/a
6 TRCN0000151177 GCAAGATAGAGTGTGTTTCAT pLKO.1 2976 3UTR 100% 5.625 2.813 Y GOLGA8F n/a
7 TRCN0000183142 GCCTATGTTCTGCTATTGTTT pLKO.1 2714 3UTR 100% 5.625 2.813 Y GOLGA8EP n/a
8 TRCN0000153701 CAAGGAGCTGAACAATGAGAA pLKO.1 1522 3UTR 100% 4.950 2.475 Y GOLGA8G n/a
9 TRCN0000157310 CAGCGCATAAGTCTCCTGAAT pLKO.1 1388 3UTR 100% 4.950 2.475 Y GOLGA8G n/a
10 TRCN0000154302 CCTCTGTTCATGGAGCTTCTT pLKO.1 3413 3UTR 100% 4.950 2.475 Y GOLGA8EP n/a
11 TRCN0000158334 CTACAGTTGGAGCAGCAAGTA pLKO.1 1553 3UTR 100% 4.950 2.475 Y GOLGA8G n/a
12 TRCN0000157512 GCTCGTTGAAGAAGGAGAAGA pLKO.1 1191 3UTR 100% 4.950 2.475 Y GOLGA8F n/a
13 TRCN0000153184 GTGGGAGTTCAAACACACAAA pLKO.1 2587 3UTR 100% 4.950 2.475 Y GOLGA8F n/a
14 TRCN0000152781 GAGAGGGATGAATATGCTGAA pLKO.1 1109 3UTR 100% 4.050 2.025 Y GOLGA8G n/a
15 TRCN0000153085 GCAACATTCATTGCAGCGTAA pLKO.1 907 3UTR 100% 4.050 2.025 Y GOLGA8IP n/a
16 TRCN0000150773 GCATGATAAATATCGGGTAGA pLKO.1 1213 3UTR 100% 4.050 2.025 Y GOLGA8G n/a
17 TRCN0000151149 GCTGTACTATTGTAGTTCCTT pLKO.1 3020 3UTR 100% 3.000 1.500 Y GOLGA8F n/a
18 TRCN0000153484 CACAGCAAATTCTTGGTGACT pLKO.1 2027 3UTR 100% 2.640 1.320 Y GOLGA8F n/a
19 TRCN0000151983 CCTCACAAACTTCTTTGTGAA pLKO.1 3754 3UTR 100% 0.495 0.248 Y GOLGA8EP n/a
20 TRCN0000154849 GAACAGCTTCAAGGAGCTGAA pLKO.1 1513 3UTR 100% 0.405 0.203 Y GOLGA8EP n/a
21 TRCN0000152586 GACACAGTTGAAGGAGTCATT pLKO.1 1072 3UTR 100% 4.950 2.475 Y GOLGA8IP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_027407.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_07828 pDONR223 100% 29.3% None (many diffs) n/a
2 ccsbBroad304_07828 pLX_304 0% 29.3% V5 (many diffs) n/a
3 TRCN0000477056 CCAAAGGCACCTCCGCCCTGATCT pLX_317 21.8% 29.3% V5 (many diffs) n/a
4 ccsbBroadEn_16170 pDONR223 0% 24.1% None (many diffs) n/a
5 ccsbBroad304_16170 pLX_304 0% 24.1% V5 (many diffs) n/a
6 ccsbBroadEn_10341 pDONR223 100% 23.9% None (many diffs) n/a
7 ccsbBroad304_10341 pLX_304 0% 23.9% V5 (many diffs) n/a
8 TRCN0000476380 AGTGATAACGTTCAAGGTCGCGGA pLX_317 28.7% 23.9% V5 (many diffs) n/a
9 ccsbBroadEn_16172 pDONR223 0% 15.8% None (many diffs) n/a
10 ccsbBroad304_16172 pLX_304 0% 15.8% V5 (many diffs) n/a
11 TRCN0000480289 TACTAACTTATTTTTTGACAAACC pLX_317 100% 3.6% V5 (not translated due to prior stop codon) 1_1015del;1209_5339del n/a
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