Construct: ORF TRCN0000478445

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005733.1_s317c1
Derived from:
ccsbBroadEn_00016
DNA Barcode:
AACAATATTTTCATGGGGTGCCTC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACTN4 (81)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478445
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 81 ACTN4 actinin alpha 4 NM_004924.6 100% 100%
2 human 81 ACTN4 actinin alpha 4 XM_006723406.3 99% 99.6% (many diffs)
3 human 81 ACTN4 actinin alpha 4 XM_005259281.5 98% 97.9% (many diffs)
4 human 81 ACTN4 actinin alpha 4 XM_017027331.2 97.6% 97.6% 2418_2483del
5 human 81 ACTN4 actinin alpha 4 NM_001322033.2 97.1% 97.5% (many diffs)
6 human 87 ACTN1 actinin alpha 1 NM_001102.4 80.2% 84.1% (many diffs)
7 human 87 ACTN1 actinin alpha 1 NM_001130004.1 78.4% 82.2% (many diffs)
8 human 87 ACTN1 actinin alpha 1 XM_017021723.2 67.6% 70.4% (many diffs)
9 human 87 ACTN1 actinin alpha 1 XM_017021722.2 67.1% 69.9% (many diffs)
10 human 87 ACTN1 actinin alpha 1 XM_017021726.2 66.3% 68.8% (many diffs)
11 human 87 ACTN1 actinin alpha 1 XM_017021725.1 65.8% 68.3% (many diffs)
12 mouse 60595 Actn4 actinin alpha 4 NM_021895.2 90.8% 98.4% (many diffs)
13 mouse 60595 Actn4 actinin alpha 4 XM_006540254.3 90.1% 98.1% (many diffs)
14 mouse 60595 Actn4 actinin alpha 4 XM_006540255.3 89.1% 96.3% (many diffs)
15 mouse 60595 Actn4 actinin alpha 4 XM_006540257.2 88.7% 96.1% (many diffs)
16 mouse 60595 Actn4 actinin alpha 4 XM_006540256.3 88.4% 96% (many diffs)
17 mouse 109711 Actn1 actinin, alpha 1 NM_134156.2 79.6% 83.9% (many diffs)
18 mouse 109711 Actn1 actinin, alpha 1 XM_006515385.2 78.2% 79.3% (many diffs)
19 mouse 109711 Actn1 actinin, alpha 1 XM_006515387.3 77.8% 81.9% (many diffs)
20 mouse 109711 Actn1 actinin, alpha 1 XM_006515384.2 76.5% 77.5% (many diffs)
21 mouse 109711 Actn1 actinin, alpha 1 XM_006515389.2 72% 76.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2799
ORF length:
2733
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt ggactaccac gcggcgaacc agtcgtacca gtacggcccc agcagcgcgg
121gcaatggcgc tggcggcggg ggcagcatgg gcgactacat ggcccaggag gacgactggg
181accgggacct gctgctggac ccggcctggg agaagcagca gcgcaagacc ttcacggcat
241ggtgcaactc ccacctgcgg aaggcaggca cacagatcga gaacattgat gaggacttcc
301gagacgggct caagctcatg ctgctcctgg aggtcatatc aggggagcgg ttacctaagc
361cggagcgggg gaagatgaga gtgcacaaaa tcaacaatgt gaacaaagcg ctggacttta
421ttgccagcaa aggcgtcaag ctggtctcca tcggggcaga agagattgtg gacggcaacg
481caaagatgac cctgggaatg atctggacca tcatccttag gttcgccatc caggacatct
541ccgtggaaga gacctcggcc aaggaagggc tccttctctg gtgccagaga aagacagccc
601cgtataagaa cgtcaatgtg cagaacttcc acatcagctg gaaggatggt cttgccttca
661atgccctgat ccaccggcac agaccagagc tgattgagta tgacaagctg aggaaggacg
721accctgtcac caacctgaac aatgccttcg aagtggctga gaaatacctc gacatcccca
781agatgctgga tgcagaggac atcgtgaaca cggcccggcc cgacgagaag gccataatga
841cctatgtgtc cagcttctac catgcctttt caggagcgca gaaggctgaa actgccgcca
901accggatctg taaggtgctg gctgtcaacc aagagaacga gcacctgatg gaggactacg
961agaagctggc cagcgacctc ctggagtgga tccggcgcac catcccctgg ctggaggacc
1021gtgtgcccca aaagactatc caggagatgc agcagaagct ggaggacttc cgcgactacc
1081ggcgtgtgca caagccgccc aaggtgcagg agaagtgcca gctggagatc aacttcaaca
1141cgctgcagac caagctgcgc ctcagcaacc ggcccgcctt catgccctcc gagggcaaga
1201tggtctcgga catcaacaat ggctggcagc acttggagca ggctgagaag ggctacgagg
1261agtggctgct gaatgagatc cgcaggctgg agcggctcga ccacctggca gagaagttcc
1321ggcagaaggc ctccatccac gaggcctgga ctgacgggaa ggaagccatg ctgaagcacc
1381gggactacga gacggccaca ctatcggaca tcaaagccct cattcgcaag cacgaggcct
1441tcgagagcga cctggctgcg caccaggacc gcgtggagca gatcgccgcc attgcccagg
1501agctcaacga gctggattac tacgactccc acaatgtcaa cacccggtgc cagaagatct
1561gtgaccagtg ggacgccctc ggctctctga cacatagtcg cagggaagcc ctggagaaaa
1621cagagaagca gctggaggcc atcgaccagc tgcacctgga atacgccaag cgcgcggccc
1681ccttcaacaa ctggatggag agcgccatgg aggacctcca ggacatgttc atcgtccata
1741ccatcgagga gattgagggc ctgatctcag cccatgacca gttcaagtcc accctgccgg
1801acgccgatag ggagcgcgag gccatcctgg ccatccacaa ggaggcccag aggatcgctg
1861agagcaacca catcaagctg tcgggcagca acccctacac caccgtcacc ccgcaaatca
1921tcaactccaa gtgggagaag gtgcagcagc tggtgccaaa acgggaccat gccctcctgg
1981aggagcagag caagcagcag tccaacgagc acctgcgccg ccagttcgcc agccaggcca
2041atgttgtggg gccctggatc cagaccaaga tggaggagat cgggcgcatc tccattgaga
2101tgaacgggac cctggaggac cagctgagcc acctgaagca gtatgaacgc agcatcgtgg
2161actacaagcc caacctggac ctgctggagc agcagcacca gctcatccag gaggccctca
2221tcttcgacaa caagcacacc aactatacca tggagcacat ccgcgtgggc tgggagcagc
2281tgctcaccac cattgcccgc accatcaacg aggtggagaa ccagatcctc acccgcgacg
2341ccaagggcat cagccaggag cagatgcagg agttccgggc gtccttcaac cacttcgaca
2401aggatcatgg cggggcgctg gggcccgagg agttcaaggc ctgcctcatc agcctgggcT
2461ACGACGTGGA GAACGACCGG CAGGGTGAGG CCGAGTTCAA CCGCATCATG AGCCTGGTCG
2521ACCCCAACCA TAGCGGCCTT GTGACCTTCC AAGCCTTCAT CGACTTCATG TCGCGGGAGA
2581CCACCGACAC GGACACGGCT GACCAGGTCA TCGCTTCCTT CAAGGTCTTA GCAGGGGACA
2641AGAACTTCAT CACAGCTGAG GAGCTGCGGA GAGAGCTGCC CCCCGACCAG GCCGAGTACT
2701GCATCGCCCG CATGGCGCCA TACCAGGGCC CTGACGCCGT GCCCGGTGCC CTCGACTACA
2761AGTCCTTCTC CACGGCCTTG TATGGCGAGA GCGACCTGTG CCCAACTTTC TTGTACAAAG
2821TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT
2881GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG
2941ACGAAACAAT ATTTTCATGG GGTGCCTCAC GCGTTAAGTC gacaatcaac ctctggatta
3001caaaatttgt gaaagatt

Download FASTA (ORF) (Full)

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