Construct: ORF TRCN0000478445
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005733.1_s317c1
- Derived from:
- ccsbBroadEn_00016
- DNA Barcode:
- AACAATATTTTCATGGGGTGCCTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTN4 (81)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000478445
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 81 | ACTN4 | actinin alpha 4 | NM_004924.6 | 100% | 100% | |
| 2 | human | 81 | ACTN4 | actinin alpha 4 | XM_006723406.3 | 99% | 99.6% | (many diffs) |
| 3 | human | 81 | ACTN4 | actinin alpha 4 | XM_005259281.5 | 98% | 97.9% | (many diffs) |
| 4 | human | 81 | ACTN4 | actinin alpha 4 | XM_017027331.2 | 97.6% | 97.6% | 2418_2483del |
| 5 | human | 81 | ACTN4 | actinin alpha 4 | NM_001322033.2 | 97.1% | 97.5% | (many diffs) |
| 6 | human | 87 | ACTN1 | actinin alpha 1 | NM_001102.4 | 80.2% | 84.1% | (many diffs) |
| 7 | human | 87 | ACTN1 | actinin alpha 1 | NM_001130004.1 | 78.4% | 82.2% | (many diffs) |
| 8 | human | 87 | ACTN1 | actinin alpha 1 | XM_017021723.2 | 67.6% | 70.4% | (many diffs) |
| 9 | human | 87 | ACTN1 | actinin alpha 1 | XM_017021722.2 | 67.1% | 69.9% | (many diffs) |
| 10 | human | 87 | ACTN1 | actinin alpha 1 | XM_017021726.2 | 66.3% | 68.8% | (many diffs) |
| 11 | human | 87 | ACTN1 | actinin alpha 1 | XM_017021725.1 | 65.8% | 68.3% | (many diffs) |
| 12 | mouse | 60595 | Actn4 | actinin alpha 4 | NM_021895.2 | 90.8% | 98.4% | (many diffs) |
| 13 | mouse | 60595 | Actn4 | actinin alpha 4 | XM_006540254.3 | 90.1% | 98.1% | (many diffs) |
| 14 | mouse | 60595 | Actn4 | actinin alpha 4 | XM_006540255.3 | 89.1% | 96.3% | (many diffs) |
| 15 | mouse | 60595 | Actn4 | actinin alpha 4 | XM_006540257.2 | 88.7% | 96.1% | (many diffs) |
| 16 | mouse | 60595 | Actn4 | actinin alpha 4 | XM_006540256.3 | 88.4% | 96% | (many diffs) |
| 17 | mouse | 109711 | Actn1 | actinin, alpha 1 | NM_134156.2 | 79.6% | 83.9% | (many diffs) |
| 18 | mouse | 109711 | Actn1 | actinin, alpha 1 | XM_006515385.2 | 78.2% | 79.3% | (many diffs) |
| 19 | mouse | 109711 | Actn1 | actinin, alpha 1 | XM_006515387.3 | 77.8% | 81.9% | (many diffs) |
| 20 | mouse | 109711 | Actn1 | actinin, alpha 1 | XM_006515384.2 | 76.5% | 77.5% | (many diffs) |
| 21 | mouse | 109711 | Actn1 | actinin, alpha 1 | XM_006515389.2 | 72% | 76.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2799
- ORF length:
- 2733
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt ggactaccac gcggcgaacc agtcgtacca gtacggcccc agcagcgcgg 121 gcaatggcgc tggcggcggg ggcagcatgg gcgactacat ggcccaggag gacgactggg 181 accgggacct gctgctggac ccggcctggg agaagcagca gcgcaagacc ttcacggcat 241 ggtgcaactc ccacctgcgg aaggcaggca cacagatcga gaacattgat gaggacttcc 301 gagacgggct caagctcatg ctgctcctgg aggtcatatc aggggagcgg ttacctaagc 361 cggagcgggg gaagatgaga gtgcacaaaa tcaacaatgt gaacaaagcg ctggacttta 421 ttgccagcaa aggcgtcaag ctggtctcca tcggggcaga agagattgtg gacggcaacg 481 caaagatgac cctgggaatg atctggacca tcatccttag gttcgccatc caggacatct 541 ccgtggaaga gacctcggcc aaggaagggc tccttctctg gtgccagaga aagacagccc 601 cgtataagaa cgtcaatgtg cagaacttcc acatcagctg gaaggatggt cttgccttca 661 atgccctgat ccaccggcac agaccagagc tgattgagta tgacaagctg aggaaggacg 721 accctgtcac caacctgaac aatgccttcg aagtggctga gaaatacctc gacatcccca 781 agatgctgga tgcagaggac atcgtgaaca cggcccggcc cgacgagaag gccataatga 841 cctatgtgtc cagcttctac catgcctttt caggagcgca gaaggctgaa actgccgcca 901 accggatctg taaggtgctg gctgtcaacc aagagaacga gcacctgatg gaggactacg 961 agaagctggc cagcgacctc ctggagtgga tccggcgcac catcccctgg ctggaggacc 1021 gtgtgcccca aaagactatc caggagatgc agcagaagct ggaggacttc cgcgactacc 1081 ggcgtgtgca caagccgccc aaggtgcagg agaagtgcca gctggagatc aacttcaaca 1141 cgctgcagac caagctgcgc ctcagcaacc ggcccgcctt catgccctcc gagggcaaga 1201 tggtctcgga catcaacaat ggctggcagc acttggagca ggctgagaag ggctacgagg 1261 agtggctgct gaatgagatc cgcaggctgg agcggctcga ccacctggca gagaagttcc 1321 ggcagaaggc ctccatccac gaggcctgga ctgacgggaa ggaagccatg ctgaagcacc 1381 gggactacga gacggccaca ctatcggaca tcaaagccct cattcgcaag cacgaggcct 1441 tcgagagcga cctggctgcg caccaggacc gcgtggagca gatcgccgcc attgcccagg 1501 agctcaacga gctggattac tacgactccc acaatgtcaa cacccggtgc cagaagatct 1561 gtgaccagtg ggacgccctc ggctctctga cacatagtcg cagggaagcc ctggagaaaa 1621 cagagaagca gctggaggcc atcgaccagc tgcacctgga atacgccaag cgcgcggccc 1681 ccttcaacaa ctggatggag agcgccatgg aggacctcca ggacatgttc atcgtccata 1741 ccatcgagga gattgagggc ctgatctcag cccatgacca gttcaagtcc accctgccgg 1801 acgccgatag ggagcgcgag gccatcctgg ccatccacaa ggaggcccag aggatcgctg 1861 agagcaacca catcaagctg tcgggcagca acccctacac caccgtcacc ccgcaaatca 1921 tcaactccaa gtgggagaag gtgcagcagc tggtgccaaa acgggaccat gccctcctgg 1981 aggagcagag caagcagcag tccaacgagc acctgcgccg ccagttcgcc agccaggcca 2041 atgttgtggg gccctggatc cagaccaaga tggaggagat cgggcgcatc tccattgaga 2101 tgaacgggac cctggaggac cagctgagcc acctgaagca gtatgaacgc agcatcgtgg 2161 actacaagcc caacctggac ctgctggagc agcagcacca gctcatccag gaggccctca 2221 tcttcgacaa caagcacacc aactatacca tggagcacat ccgcgtgggc tgggagcagc 2281 tgctcaccac cattgcccgc accatcaacg aggtggagaa ccagatcctc acccgcgacg 2341 ccaagggcat cagccaggag cagatgcagg agttccgggc gtccttcaac cacttcgaca 2401 aggatcatgg cggggcgctg gggcccgagg agttcaaggc ctgcctcatc agcctgggcT 2461 ACGACGTGGA GAACGACCGG CAGGGTGAGG CCGAGTTCAA CCGCATCATG AGCCTGGTCG 2521 ACCCCAACCA TAGCGGCCTT GTGACCTTCC AAGCCTTCAT CGACTTCATG TCGCGGGAGA 2581 CCACCGACAC GGACACGGCT GACCAGGTCA TCGCTTCCTT CAAGGTCTTA GCAGGGGACA 2641 AGAACTTCAT CACAGCTGAG GAGCTGCGGA GAGAGCTGCC CCCCGACCAG GCCGAGTACT 2701 GCATCGCCCG CATGGCGCCA TACCAGGGCC CTGACGCCGT GCCCGGTGCC CTCGACTACA 2761 AGTCCTTCTC CACGGCCTTG TATGGCGAGA GCGACCTGTG CCCAACTTTC TTGTACAAAG 2821 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 2881 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 2941 ACGAAACAAT ATTTTCATGG GGTGCCTCAC GCGTTAAGTC gacaatcaac ctctggatta 3001 caaaatttgt gaaagatt