Construct: ORF TRCN0000478521

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015782.1_s317c1
Derived from:
ccsbBroadEn_12837
DNA Barcode:
CACCAGGACAGGTCCAATTAGTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
GLYR1 (84656)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000478521
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84656 GLYR1 glyoxylate reductase 1 homolog XM_011522717.2 99.9% 100% 1413T>C
2 human 84656 GLYR1 glyoxylate reductase 1 homolog XM_017023788.2 98.6% 98.7% 699_700insTGTGATTTGTTCATCCAG;1395T>C
3 human 84656 GLYR1 glyoxylate reductase 1 homolog XM_017023789.2 96.4% 96.4% 472_473ins51;1362T>C
4 human 84656 GLYR1 glyoxylate reductase 1 homolog XM_011522716.3 88.2% 88.3% 1_192del;1605T>C
5 human 84656 GLYR1 glyoxylate reductase 1 homolog NM_032569.4 87.4% 87.5% 1_207del;1620T>C
6 human 84656 GLYR1 glyoxylate reductase 1 homolog NM_001308096.1 86.3% 86.4% 1_207del;906_907insTGTGATTTGTTCATCCAG;1602T>C
7 human 84656 GLYR1 glyoxylate reductase 1 homolog NM_001324098.2 84.3% 84.4% 1_207del;679_680ins51;1569T>C
8 human 84656 GLYR1 glyoxylate reductase 1 homolog XM_017023786.2 80% 80% 1_357del;1167_1168insCTG;1767T>C
9 human 84656 GLYR1 glyoxylate reductase 1 homolog XM_017023787.2 76.3% 76.4% (many diffs)
10 human 84656 GLYR1 glyoxylate reductase 1 homolog NM_001324097.2 72.8% 72.8% 1_207del;292_293ins243;1377T>C
11 human 84656 GLYR1 glyoxylate reductase 1 homolog NM_001324096.2 71.7% 71.7% (many diffs)
12 human 84656 GLYR1 glyoxylate reductase 1 homolog XM_005255639.5 63.9% 64% (many diffs)
13 human 84656 GLYR1 glyoxylate reductase 1 homolog XR_001752010.2 47.3% 1_974del;1785_1901del;2294_2295ins249
14 human 84656 GLYR1 glyoxylate reductase 1 homolog NR_136697.1 42.1% 1_330del;1743T>C;1783_3445del
15 human 84656 GLYR1 glyoxylate reductase 1 homolog NR_136696.1 41.5% (many diffs)
16 human 84656 GLYR1 glyoxylate reductase 1 homolog NR_136695.2 40.9% 1_429del;1842T>C;1882_3544del
17 human 84656 GLYR1 glyoxylate reductase 1 homolog NR_136699.1 40.6% (many diffs)
18 human 84656 GLYR1 glyoxylate reductase 1 homolog NR_136698.1 40.1% (many diffs)
19 human 84656 GLYR1 glyoxylate reductase 1 homolog XR_002957846.1 37.7% (many diffs)
20 human 84656 GLYR1 glyoxylate reductase 1 homolog NR_136700.1 35.5% (many diffs)
21 human 84656 GLYR1 glyoxylate reductase 1 homolog XR_001752009.2 33.4% (many diffs)
22 human 84656 GLYR1 glyoxylate reductase 1 homolog XR_001752008.2 30.7% (many diffs)
23 mouse 74022 Glyr1 glyoxylate reductase 1 homo... XM_006522642.3 92.5% 98.3% (many diffs)
24 mouse 74022 Glyr1 glyoxylate reductase 1 homo... XM_017317142.1 91.3% 97.1% (many diffs)
25 mouse 74022 Glyr1 glyoxylate reductase 1 homo... NM_001079814.1 81% 86% (many diffs)
26 mouse 74022 Glyr1 glyoxylate reductase 1 homo... NM_028720.2 79.9% 84.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1518
ORF length:
1452
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgat aaaaattaac aagggtaaac gattccagca agcggtagat gctgtcgaag
121agttcctcag gagagccaaa gggaaagacc agacgtcatc ccacaattct tctgatgaca
181agaatcgacg taattccagt gaggagagaa gtaggccaaa ctcaggtgat gagaagcgca
241aacttagcct gtctgaaggg aaggtgaaga agaacatggg agaaggaaag aagagggtgt
301cttcaggctc ttcagagaga ggctccaaat cccctctgaa aagagcccaa gagcaaagtc
361cccggaagcg gggtcggccc ccaaaggatg agaaggatct caccatcccg gagtctagta
421ccgtgaaggg gatgatggcc ggaccgatgg ccgcgtttaa atggcagcca accgcaagcg
481agcctgttaa agatgcagat cctcatttcc atcatttcct gctaagccaa acagagaagc
541cagctgtctg ttaccaggca atcacgaaga agttgaaaat atgtgaagag gaaactggct
601ccacctccat ccaggcagct gacagcacag ccgtgaatgg cagcatcaca cccacagaca
661aaaagatagg atttttgggc cttggtctca tgggaagtgg aatcgtctcc aacttgctaa
721aaatgggtca cacagtgact gtctggaacc gcactgcaga gaaatgtgat ttgttcatcc
781aggagggggc ccgtctggga agaacccccg ctgaagtcgt ctcaacctgc gacatcactt
841tcgcctgcgt gtcggatccc aaggcggcca aggacctggt gctgggcccc agtggtgtgc
901tgcaagggat ccgccctggg aagtgctacg tggacatgtc aacagtggac gctgacaccg
961tcactgagct ggcccaggtg attgtgtcca ggggggggcg ctttctggaa gcccccgtct
1021cagggaatca gcagctgtct aatgacggga tgttggtgat cttagcggct ggagacaggg
1081gcttatatga ggactgcagc agctgcttcc aggcgatggg gaagacctcc ttcttccTAG
1141GTGAAGTGGG CAATGCAGCC AAGATGATGC TGATCGTGAA CATGGTCCAA GGGAGCTTCA
1201TGGCCACTAT TGCCGAGGGG CTGACCCTGG CCCAGGTGAC AGGCCAGTCC CAGCAGACAC
1261TCTTGGACAT CCTCAATCAG GGACAGTTGG CCAGCATCTT CCTGGACCAG AAGTGCCAAA
1321ATATCCTGCA AGGAAACTTT AAGCCTGATT TCTACCTGAA ATACATTCAG AAGGATCTCC
1381GCTTAGCCAT TGCGCTGGGT GATGCGGTCA ACCATCCGAC TCCCATGGCA GCTGCAGCAA
1441ATGAGGTGTA CAAAAGAGCC AAGGCGCTGG ACCAGTCCGA CAACGATATG TCCGCCGTGT
1501ACCGAGCCTA CATACACTAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA
1561TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA
1621GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACACCAGG ACAGGTCCAA
1681TTAGTTTACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt

Download FASTA (ORF) (Full)