Construct: ORF TRCN0000479556
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008490.2_s317c1
- Derived from:
- ccsbBroadEn_07799
- DNA Barcode:
- ACGAAAGCCTCATCTGGTGCATAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- U2AF2 (11338)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000479556
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11338 | U2AF2 | U2 small nuclear RNA auxili... | NM_001012478.1 | 100% | 100% | |
| 2 | human | 11338 | U2AF2 | U2 small nuclear RNA auxili... | NM_007279.2 | 99.1% | 99.1% | 1032_1043del |
| 3 | human | 11338 | U2AF2 | U2 small nuclear RNA auxili... | XM_011526410.1 | 77.1% | 77.2% | 0_1ins312;720_731del;891C>T |
| 4 | human | 11338 | U2AF2 | U2 small nuclear RNA auxili... | XM_006722994.2 | 64.5% | 64.6% | 0_1ins492;540_551del;711C>T |
| 5 | mouse | 22185 | U2af2 | U2 small nuclear ribonucleo... | NM_133671.3 | 91.2% | 100% | (many diffs) |
| 6 | mouse | 22185 | U2af2 | U2 small nuclear ribonucleo... | NM_001205231.1 | 90.5% | 99.1% | (many diffs) |
| 7 | mouse | 22185 | U2af2 | U2 small nuclear ribonucleo... | XM_006539728.3 | 88.1% | 96.8% | (many diffs) |
| 8 | mouse | 22185 | U2af2 | U2 small nuclear ribonucleo... | XM_006539727.3 | 87.4% | 96% | (many diffs) |
| 9 | mouse | 22185 | U2af2 | U2 small nuclear ribonucleo... | XM_017322124.1 | 70.5% | 77.2% | (many diffs) |
| 10 | mouse | 22185 | U2af2 | U2 small nuclear ribonucleo... | XM_006539730.2 | 58.8% | 64.6% | (many diffs) |
| 11 | mouse | 102640468 | LOC102640468 | splicing factor U2AF 65 kDa... | XR_001782861.1 | 30.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1479
- ORF length:
- 1413
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc ggacttcgac gagttcgagc ggcagctcaa cgagaataaa caagagcggg 121 acaaggagaa ccggcatcgg aagcgcagcc acagccgctc tcggagccgg gaccgcaaac 181 gccggagccg gagccgcgac cggcgcaacc gggaccagcg gagcgcctcc cgggacaggc 241 gacgacgcag caaacctttg accagaggcg ctaaagagga gcacggtgga ctgattcgtt 301 ccccccgcca cgagaagaag aagaaggtcc gtaaatactg ggacgtgcca cccccaggct 361 ttgagcacat caccccaatg cagtacaagg ccatgcaagc tgcgggtcag attccagcca 421 ctgctcttct ccccaccatg acccctgacg gtctggctgt gaccccaacg ccggtgcccg 481 tggtcgggag ccagatgacc agacaagccc ggcgcctcta cgtgggcaac atcccctttg 541 gcatcactga ggaggccatg atggatttct tcaacgccca gatgcgcctg ggggggctga 601 cccaggcccc tggcaaccca gtgttggctg tgcagattaa ccaggacaag aattttgcct 661 ttttggagtt ccgctcagtg gacgagacta cccaggctat ggcctttgat ggcatcatct 721 tccagggcca gtcactaaag atccgcaggc ctcacgacta ccagccgctt cctggcatgt 781 cagagaaccc ctccgtctat gtgcctgggg ttgtgtccac tgtggtcccc gactctgccc 841 acaagctgtt catcgggggc ttacccaact acctgaacga tgaccaggtc aaagagctgc 901 tgacatcctt tgggcccctc aaggccttca acctggtcaa ggacagtgcc acggggctct 961 ccaagggcta cgccttctgt gagtacgtgg acatcaacgt cacggatcag gccattgcgg 1021 ggctgaacgg catgcagctg ggggataaga agctgctggt ccagagggcg agtgtgggag 1081 ccaagaatgc cacgctgagc accatcaatc agacgcctgt gaccctgcaa gtgccgggct 1141 tgatgagctc ccaggtgcag atgggcggcc acccgactga ggtcctgtgc cTCATGAACA 1201 TGGTGCTGCC TGAGGAGCTG CTGGACGACG AGGAGTATGA GGAGATCGTG GAGGATGTGC 1261 GGGACGAGTG CAGCAAGTAC GGGCTTGTCA AGTCCATCGA GATCCCCCGG CCTGTGGACG 1321 GCGTCGAGGT GCCCGGCTGC GGAAAGATCT TTGTGGAGTT CACCTCTGTG TTTGACTGCC 1381 AGAAAGCCAT GCAGGGCCTG ACGGGCCGCA AGTTCGCCAA CAGAGTGGTT GTCACAAAAT 1441 ACTGTGACCC CGACTCTTAT CACCGCCGGG ACTTCTGGTA CCCAACTTTC TTGTACAAAG 1501 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 1561 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG 1621 ACGAACGAAA GCCTCATCTG GTGCATACAC GCGTTAAGTC gacaatcaac ctctggatta 1681 caaaatttgt gaaagatt