Construct: ORF TRCN0000480164

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000654.1_s317c1
Derived from:
ccsbBroadEn_04404
DNA Barcode:
TGTCGAGACCCGACGGGAATTTTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TUBB6 (84617)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000480164
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84617 TUBB6 tubulin beta 6 class V NM_001303524.1 100% 100%
2 human 84617 TUBB6 tubulin beta 6 class V NM_032525.3 100% 100%
3 human 84617 TUBB6 tubulin beta 6 class V NM_001303526.2 91.7% 91.4% 166_167ins111
4 human 10381 TUBB3 tubulin beta 3 class III NM_006086.4 87.7% 91.5% (many diffs)
5 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289129.1 86.3% 91.7% (many diffs)
6 human 10382 TUBB4A tubulin beta 4A class IVa NM_006087.4 86.3% 91.7% (many diffs)
7 human 7280 TUBB2A tubulin beta 2A class IIa NM_001069.3 86.2% 90.5% (many diffs)
8 human 347733 TUBB2B tubulin beta 2B class IIb NM_178012.5 86.1% 90.8% (many diffs)
9 human 84617 TUBB6 tubulin beta 6 class V NM_001303528.2 83.8% 77.7% (many diffs)
10 human 84617 TUBB6 tubulin beta 6 class V NM_001303527.2 83.8% 83.8% 0_1ins216
11 human 10383 TUBB4B tubulin beta 4B class IVb NM_006088.6 83.2% 90.5% (many diffs)
12 human 203068 TUBB tubulin beta class I NM_178014.4 80.9% 90.3% (many diffs)
13 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289127.1 78.5% 83.3% (many diffs)
14 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289123.1 77.5% 82.3% (many diffs)
15 human 203068 TUBB tubulin beta class I NM_001293212.2 75.9% 83.6% (many diffs)
16 human 10381 TUBB3 tubulin beta 3 class III NM_001197181.2 74.7% 77.3% (many diffs)
17 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289130.1 74.2% 78.2% (many diffs)
18 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289131.1 74.2% 78.2% (many diffs)
19 human 203068 TUBB tubulin beta class I NM_001293214.2 74% 82.9% (many diffs)
20 human 7280 TUBB2A tubulin beta 2A class IIa NM_001310315.2 71.1% 72.6% (many diffs)
21 human 203068 TUBB tubulin beta class I NM_001293215.2 69% 76.9% (many diffs)
22 human 203068 TUBB tubulin beta class I NM_001293216.2 69% 76.9% (many diffs)
23 human 84617 TUBB6 tubulin beta 6 class V NM_001303529.2 67.2% 67.2% 0_1ins438
24 human 84617 TUBB6 tubulin beta 6 class V NM_001303530.2 67.2% 67.2% 0_1ins438
25 human 347688 TUBB8 tubulin beta 8 class VIII XM_011519459.3 67% 72.4% (many diffs)
26 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016192.2 59.9% 63.9% (many diffs)
27 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016193.2 59.9% 63.9% (many diffs)
28 human 260334 TUBB8B tubulin beta 8B XM_024451147.1 59% 63.2% (many diffs)
29 human 203068 TUBB tubulin beta class I NM_001293213.2 43.6% 47% (many diffs)
30 human 84617 TUBB6 tubulin beta 6 class V NM_001303525.1 22.5% 20.8% (many diffs)
31 mouse 67951 Tubb6 tubulin, beta 6 class V NM_026473.2 89.7% 97.3% (many diffs)
32 mouse 22152 Tubb3 tubulin, beta 3 class III NM_023279.2 85.8% 91.3% (many diffs)
33 mouse 73710 Tubb2b tubulin, beta 2B class IIB NM_023716.2 84.7% 90.8% (many diffs)
34 mouse 22151 Tubb2a tubulin, beta 2A class IIA NM_009450.2 83.3% 90.5% (many diffs)
35 mouse 22153 Tubb4a tubulin, beta 4A class IVA NM_009451.3 82.1% 91.7% (many diffs)
36 mouse 22154 Tubb5 tubulin, beta 5 class I NM_011655.5 80.5% 90.3% (many diffs)
37 mouse 227613 Tubb4b tubulin, beta 4B class IVB NM_146116.2 80.2% 90.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1404
ORF length:
1338
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag ggagatcgtg cacatccagg cgggccagtg cgggaaccag atcggcacca
121agttttggga agtgatcagc gatgagcacg gcatcgaccc ggccggaggc tacgtgggag
181actcggcgct gcagctggag agaatcaacg tctactacaa tgagtcatcg tctcagaaat
241atgtgcccag ggccgccctg gtggacttag agccaggcac catggacagc gtgcggtctg
301ggccttttgg gcagcttttc cggcctgaca acttcatctt tggccagacg ggtgcaggga
361acaactgggc gaaagggcac tacacggagg gcgcggagct ggtggacgca gtgctggacg
421tggtgcggaa ggagtgcgag cactgcgact gcctgcaggg cttccagctc acgcactcgc
481tgggcggcgg cacgggctca ggcatgggca cgctgctcat cagcaagatc cgtgaggagt
541tcccggaccg catcatgaac accttcagcg tcatgccctc gcccaaggtg tcggacacgg
601tggtggagcc ctacaatgcc acactgtcgg tgcaccagct ggtggagaat acagacgaga
661cctactgcat cgacaacgag gcgctctatg acatctgctt ccgcactctg aagctgacaa
721cgcccaccta cggggacctc aaccacctgg tgtccgccac catgagtggg gtcaccacct
781cgctgcgctt cccgggccag ctcaatgctg acctgcgcaa gctggcggtg aacatggtgc
841ccttcccgcg cctgcacttc ttcatgcctg gcttcgcgcc gctcaccagc cgcggcagcc
901agcagtaccg ggccctgacc gtgcccgagc tcacccagca gatgttcgac gccaggaaca
961tgatggccgc ctgcgatccg cgccatggcc gctacctgac cgtggccacc gtgttccgcg
1021ggcccatgtc catgaaggag gtggacgagc agatgctggc catccagagt aagaacagca
1081gcTACTTCGT GGAGTGGATT CCCAACAACG TGAAGGTGGC CGTGTGCGAC ATCCCGCCCC
1141GCGGCCTGAA GATGGCCTCC ACCTTCATCG GCAACAGCAC GGCCATCCAG GAGCTGTTCA
1201AGCGCATCTC CGAGCAGTTC TCAGCCATGT TCCGGCGCAA GGCCTTCCTG CACTGGTTCA
1261CGGGTGAGGG CATGGATGAA ATGGAGTTCA CCGAGGCGGA GAGCAACATG AACGACCTGG
1321TATCCGAGTA CCAGCAGTAC CAGGATGCCA CCGCCAATGA CGGGGAGGAA GCTTTTGAGG
1381ATGAGGAAGA GGAGATCGAT GGATACCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1441AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1501TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT GTCGAGACCC
1561GACGGGAATT TTTACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1621att

Download FASTA (ORF) (Full)