Construct: ORF TRCN0000480649
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003188.2_s317c1
- Derived from:
- ccsbBroadEn_13979
- DNA Barcode:
- GTATCCTGATATGCGTTGCTGGGC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- UGT2B7 (7364)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000480649
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 7364 | UGT2B7 | UDP glucuronosyltransferase... | NM_001074.4 | 99.9% | 99.2% | 1573delA |
| 2 | human | 7365 | UGT2B10 | UDP glucuronosyltransferase... | NM_001075.6 | 92.5% | 86.9% | (many diffs) |
| 3 | human | 7365 | UGT2B10 | UDP glucuronosyltransferase... | XM_017008585.2 | 92% | 86.5% | (many diffs) |
| 4 | human | 10720 | UGT2B11 | UDP glucuronosyltransferase... | NM_001073.3 | 91.7% | 85% | (many diffs) |
| 5 | human | 54490 | UGT2B28 | UDP glucuronosyltransferase... | NM_053039.2 | 91.3% | 84.4% | (many diffs) |
| 6 | human | 7363 | UGT2B4 | UDP glucuronosyltransferase... | NM_021139.3 | 90.3% | 84.5% | (many diffs) |
| 7 | human | 7367 | UGT2B17 | UDP glucuronosyltransferase... | NM_001077.3 | 85.5% | 76.4% | (many diffs) |
| 8 | human | 7367 | UGT2B17 | UDP glucuronosyltransferase... | XM_024454205.1 | 85.5% | 76.4% | (many diffs) |
| 9 | human | 7366 | UGT2B15 | UDP glucuronosyltransferase... | NM_001076.4 | 84.7% | 77.5% | (many diffs) |
| 10 | human | 7365 | UGT2B10 | UDP glucuronosyltransferase... | NM_001144767.3 | 77.7% | 72.7% | (many diffs) |
| 11 | human | 10720 | UGT2B11 | UDP glucuronosyltransferase... | XM_011531550.2 | 77.4% | 72.2% | (many diffs) |
| 12 | human | 7364 | UGT2B7 | UDP glucuronosyltransferase... | NM_001330719.1 | 69.4% | 68.6% | (many diffs) |
| 13 | human | 7363 | UGT2B4 | UDP glucuronosyltransferase... | NM_001297616.1 | 67.5% | 62.1% | (many diffs) |
| 14 | human | 54490 | UGT2B28 | UDP glucuronosyltransferase... | XM_017008303.1 | 67% | 61.4% | (many diffs) |
| 15 | human | 7363 | UGT2B4 | UDP glucuronosyltransferase... | NM_001297615.2 | 62% | 57.4% | (many diffs) |
| 16 | human | 54490 | UGT2B28 | UDP glucuronosyltransferase... | NM_001207004.2 | 56.8% | 51.2% | (many diffs) |
| 17 | human | 7364 | UGT2B7 | UDP glucuronosyltransferase... | NM_001349568.1 | 52.8% | 52.1% | 0_1ins747;826delA |
| 18 | human | 7365 | UGT2B10 | UDP glucuronosyltransferase... | NM_001290091.2 | 50.1% | 48.5% | (many diffs) |
| 19 | human | 10720 | UGT2B11 | UDP glucuronosyltransferase... | XM_017007660.2 | 49.6% | 47.6% | (many diffs) |
| 20 | human | 101929773 | LOC101929773 | UDP-glucuronosyltransferase... | XM_005275640.3 | 34.7% | 33.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1653
- ORF length:
- 1587
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc tgtgaaatgg acttcagtaa ttttgctaat acaactgagc ttttgcttta 121 gctctgggaa ttgtggaaag gtgctggtgt gggcagcaga atacagccat tggatgaata 181 taaagacaat cctggatgag cttattcaga gaggtcatga ggtgactgta ctggcatctt 241 cagcttccat tctttttgat cccaacaact catccgctct taaaattgaa atttatccca 301 catctttaac taaaactgag ttggagaatt tcatcatgca acagattaag agatggtcag 361 accttccaaa agatacattt tggttatatt tttcacaagt acaggaaatc atgtcaatat 421 ttggtgacat aactagaaag ttctgtaaag atgtagtttc aaataagaaa tttatgaaaa 481 aagtacaaga gtcaagattt gacgtcattt ttgcagatgc tatttttccc tgtagtgagc 541 tgctggctga gctatttaac ataccctttg tgtacagtct cagcttctct cctggctaca 601 cttttgaaaa gcatagtgga ggatttattt tccctccttc ctacgtacct gttgttatgt 661 cagaattaac tgatcaaatg actttcatgg agagggtaaa aaatatgatc tatgtgcttt 721 actttgactt ttggttcgaa atatttgaca tgaagaagtg ggatcagttt tatagtgaag 781 ttctaggaag acccactaca ttatctgaga caatggggaa agctgacgta tggcttattc 841 gaaactcctg gaattttcag tttccatatc cactcttacc aaatgttgat tttgttggag 901 gactccactg caaacctgcc aaacccctgc ctaaggaaat ggaagacttt gtacagagct 961 ctggagaaaa tggtgttgtg gtgttttctc tggggtcaat ggtcagtaac atgacagaag 1021 aaagggccaa cgtaattgca tcagccctgg cccagatccc acaaaaggtt ctgtggagat 1081 ttgatgggaa taaaccagat accttaggtc tcaatactcg gctctacaag tggatacccc 1141 agaatgacct tctaggtcat ccaaagacca gagcttttat aactcatggt ggagccaatg 1201 gcatctacga ggcaatctac catgggatcc cTATGGTGGG GATTCCATTG TTTGCCGATC 1261 AACCTGATAA CATTGCTCAC ATGAAGGCCA GGGGAGCAGC TGTTAGAGTG GACTTCAACA 1321 CAATGTCGAG TACAGACTTG CTGAATGCAT TGAAGAGAGT AATTAATGAT CCTTCATATA 1381 AAGAGAATGT TATGAAATTA TCAAGAATTC AACATGATCA ACCAGTGAAG CCCCTGGATC 1441 GAGCAGTCTT CTGGATTGAA TTTGTCATGC GCCACAAAGG AGCTAAACAC CTTCGGGTTG 1501 CAGCCCACGA CCTCACCTGG TTCCAGTACC ACTCTTTGGA TGTGATTGGG TTCCTGCTGG 1561 TCTGTGTGGC AACTGTGATA TTTATCGTCA CAAAATGTTG TCTGTTTTGT TTCTGGAAGT 1621 TTGCTAGAAA AGCAAAGAGG GAAAAAATGA TTACCCAACT TTCTTGTACA AAGTGGTTGA 1681 TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG 1741 TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGTA 1801 TCCTGATATG CGTTGCTGGG CACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt 1861 tgtgaaagat t