Construct: ORF TRCN0000489060

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF021400.1_s317c1
DNA Barcode:
TGCGAAAACAAGTACTTATGAAAC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
SIK3 (23387)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000489060
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23387 SIK3 SIK family kinase 3 NM_001366686.2 95.5% 95.6% (many diffs)
2 human 23387 SIK3 SIK family kinase 3 XM_011542723.2 92.1% 92.1% (many diffs)
3 human 23387 SIK3 SIK family kinase 3 XM_011542724.2 92.1% 92.1% (many diffs)
4 human 23387 SIK3 SIK family kinase 3 XM_011542725.2 92.1% 92.1% (many diffs)
5 human 23387 SIK3 SIK family kinase 3 XM_017017425.1 92.1% 92.1% (many diffs)
6 human 23387 SIK3 SIK family kinase 3 XM_017017426.1 92.1% 92.1% (many diffs)
7 human 23387 SIK3 SIK family kinase 3 XM_005271482.4 92% 92.1% (many diffs)
8 human 23387 SIK3 SIK family kinase 3 NM_025164.6 92% 92% (many diffs)
9 human 23387 SIK3 SIK family kinase 3 XM_011542722.1 91.1% 91.2% (many diffs)
10 human 23387 SIK3 SIK family kinase 3 NM_001281749.3 87.6% 87.7% (many diffs)
11 human 23387 SIK3 SIK family kinase 3 XM_005271484.3 87.6% 87.6% (many diffs)
12 human 23387 SIK3 SIK family kinase 3 XM_017017424.1 84.1% 84.1% (many diffs)
13 human 23387 SIK3 SIK family kinase 3 NM_001281748.3 83.8% 83.8% (many diffs)
14 human 23387 SIK3 SIK family kinase 3 XM_005271485.3 58.3% 58.2% (many diffs)
15 human 23387 SIK3 SIK family kinase 3 XM_017017427.1 54.7% 54.6% (many diffs)
16 human 23387 SIK3 SIK family kinase 3 XR_001747816.1 46.5% (many diffs)
17 human 23387 SIK3 SIK family kinase 3 XM_011542726.1 43.5% 43.6% (many diffs)
18 human 23387 SIK3 SIK family kinase 3 NM_001366687.1 11.5% 11.1% (many diffs)
19 mouse 70661 Sik3 SIK family kinase 3 NM_027498.3 84.8% 87.8% (many diffs)
20 mouse 70661 Sik3 SIK family kinase 3 XM_006510590.2 81.5% 84.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
90
ORF end:
4023
ORF length:
3933
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg
61cggccgcccc cttcaccgaa ttcggaccca tgcccgcccg tatcggctac tacgagatcg
121accgcaccat cggcaagggc aacttcgcgg tggtcaagcg ggccacgcac ctcgtcacca
181aggccaaggt tgctatcaag atcatagata agacccagct ggatgaagaa aacttgaaga
241agattttccg ggaagttcaa attatgaaga tgctttgcca cccccatatc atcaggctct
301accaggttat ggagacagaa cggatgattt atctggtgac agaatatgct agtggagggg
361aaatatttga ccacctggtg gcccatggta gaatggcaga aaaggaggca cgtcggaagt
421tcaaacagat cgtcacagct gtctattttt gtcactgtcg gaacattgtt catcgtgatt
481taaaagctga aaatttactt ctggatgcca atctgaatat caaaatagca gattttggtt
541tcagtaacct cttcactcct gggcagctgc tgaagacctg gtgtggcagc cctccctatg
601ctgcacctga actctttgaa ggaaaagaat atgatgggcc caaagtggac atctggagcc
661ttggagttgt cctctacgtg cttgtgtgcg gtgccctgcc atttgatgga agcacactgc
721agaatctgcg ggcccgcgtg ctgagtggaa agttccgcat cccatttttt atgtccacag
781aatgtgagca tttgatccgc catatgttgg tgttagatcc caataagcgc ctctccatgg
841agcagatctg caagcacaag tggatgaagc taggggacgc cgatcccaac tttgacaggt
901taatagctga atgccaacaa ctaaaggaag aaagacaggt ggaccccctg aatgaggatg
961tcctcttggc catggaggac atgggactgg acaaagaaca gacactgcag tcattaagat
1021cagatgccta tgatcactat agtgcaatct acagcctgct gtgtgatcga cataagagac
1081ataaaaccct gcgtctcgga gcacttccta gcatgccccg agccctggcc tttcaagcac
1141cagtcaatat ccaggcggag caggcaggta ctgctatgaa catcagcgtt ccccaggtgc
1201agctgatcaa cccagagaac caaattgtgg agccggatgg gacactgaat ttggacagtg
1261atgagggtga agagccttcc cctgaagcat tggtgcgcta tttgtcaatg aggaggcaca
1321cagtgggtgt ggctgaccca cgcacggaag ttatggaaga tctgcagaag ctcctacctg
1381gctttcctgg agtcaacccc caggctccat tcctgcaggt ggcccctaat gtgaacttca
1441tgcacaacct gttgcctatg caaaacttgc aaccaaccgg gcaacttgag tacaaggagc
1501agtctctcct acagccgccc acgctacagc tgttgaatgg aatgggcccc cttggccgga
1561gggcatcaga tggaggagcc aacatccaac tgcatgccca gcagctgctg aagcgcccac
1621ggggaccctc tccgcttgtc accatgacac cagcagtgcc agcagttacc cctgtggacg
1681aggagagctc agacggggag ccagaccagg aagctgtgca gaggtacttg gcaaataggt
1741ccaaaagaca tacactggcc atgaccaacc ctacagctga gatcccaccg gacctacaac
1801ggcagctagg acagcagcct ttccgttccc gggtctggcc tcctcacctg gtacctgatc
1861agcatcgctc tacctacaag gactccaaca ctctgcacct ccctacggag cgtttctccc
1921ctgtgcgccg gttctcagat ggggctgcga gcatccaggc cttcaaagct cacctggaaa
1981aaatgggcaa caacagcagc atcaaacagc tgcagcagga gtgtgagcag ctgcagaaga
2041tgtacggggg gcagattgat gaaagaaccc tggagaagac ccagcagcag catatgttat
2101accagcagga gcagcaccat caaattctcc agcaacaaat tcaagactct atctgtcctc
2161ctcagccatc tccacctctt caggctgcat gtgaaaatca gccagccctc cttacccatc
2221agctccagag gttaaggatt cagccttcaa gcccaccccc caaccacccc aacaaccatc
2281tcttcaggca gcccagtaat agtcctcccc ccatgagcag tgccatgatc cagcctcacg
2341gggctgcatc ttcttcccag tttcaaggct taccttcccg cagtgcaatc tttcagcagc
2401aacctgagaa ctgttcctct cctcccaacg tggcactaac ctgcttgggt atgcagcagc
2461ctgctcagtc acagcaggtc accatccaag tccaagagcc tgttgacatg ctcagcaaca
2521tgccaggcac agctgcaggc tccagtgggc gcggcatctc catcagcccc agtgctggtc
2581agatgcagat gcagcaccgt accaacctga tggccaccct cagctatggg caccgtccct
2641tgtccaagca gctgagtgct gacagtgcag aggctcacag cttgaacgtg aatcggttct
2701cccctgctaa ctacgaccag gcgcatttac acccccatct gttttcggac cagtcccggg
2761gttcccccag cagctacagc ccttcaacag gagtggggtt ctctccaacc caagccctga
2821aagtccctcc acttgaccaa ttccccacct tccctcccag tgcacatcag cagccgccac
2881actataccac gtcggcacta cagcaggccc tgctgtctcc cacgccgcca gactatacaa
2941gacaccagca ggtaccccac atccttcaag gactgctttc tccccggcat tcgctcaccg
3001gccactcgga catccggctg cccccaacag agtttgcaca gctcattaaa aggcagcagc
3061aacaacggca gcagcagcag caacagcagc aacagcaaga ataccaggaa ctgttcaggc
3121acatgaacca aggggatgcg gggagtctgg ctcccagcct tgggggacag agcatgacag
3181agcgccaggc tttatcttat caaaatgctg actcttatca ccatcacacc agcccccagc
3241atctgctaca aatcagggca caagaatgtg tctcacaggc ttcctcaccc accccgcccc
3301acgggtatgc tcaccagccg gcactgatgc attcagagag catggaggag gactgctcgt
3361gtgagggggc caaggatggc ttccaagaca gtaagagttc aagtacattg accaaaggtt
3421gccatgacag ccctctgctc ttgagtaccg gtggacctgg ggaccgtgaa tctttgctag
3481gaactgtgag tcatgcccaa gaattgggga tacatcccta tggtcatcag ccaactgctg
3541cattcagtaa aaataaggtg cccagcagag agcctgtcat agggaactgc atggatagaa
3601gttctccagg acaagcagtg gagctgccgg atcacaatgg gctcgggtac ccagcacgcc
3661cctccgtcca tgagcaccac aggccccggg CCCTCCAGAG ACACCACACG ATCCAGAACA
3721GCGACGATGC TTATGTACAG CTGGATAACT TGCCAGGAAT GAGTCTCGTG GCTGGGAAAG
3781CACTTAGCTC TGCCCGGATG TCGGATGCAG TTCTCAATCA GTCTTCGCTC ATGGGCAGCC
3841AGCAGTTTCA GGATGGGGAA AATGAGGAAT GTGGGGCAAG CCTGGGAGGT CATGAGCACC
3901CAGACCTGAG TGATGGCAGC CAGCATTTAA ACTCCTCTTG CTATCCATCT ACGTGTATTA
3961CAGACATTCT GCTCAGCTAC AAGCACCCCG AAGTCTCCTT CAGCATGGAG CAGGCAGGCG
4021TGTAAGCTTA AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT
4081AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA
4141CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGCGAAAACA
4201AGTACTTATG AAACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa
4261gatt

Download FASTA (ORF) (Full)

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