Construct: ORF TRCN0000489060
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF021400.1_s317c1
- DNA Barcode:
- TGCGAAAACAAGTACTTATGAAAC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- SIK3 (23387)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000489060
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23387 | SIK3 | SIK family kinase 3 | NM_001366686.2 | 95.5% | 95.6% | (many diffs) |
| 2 | human | 23387 | SIK3 | SIK family kinase 3 | XM_011542723.2 | 92.1% | 92.1% | (many diffs) |
| 3 | human | 23387 | SIK3 | SIK family kinase 3 | XM_011542724.2 | 92.1% | 92.1% | (many diffs) |
| 4 | human | 23387 | SIK3 | SIK family kinase 3 | XM_011542725.2 | 92.1% | 92.1% | (many diffs) |
| 5 | human | 23387 | SIK3 | SIK family kinase 3 | XM_017017425.1 | 92.1% | 92.1% | (many diffs) |
| 6 | human | 23387 | SIK3 | SIK family kinase 3 | XM_017017426.1 | 92.1% | 92.1% | (many diffs) |
| 7 | human | 23387 | SIK3 | SIK family kinase 3 | XM_005271482.4 | 92% | 92.1% | (many diffs) |
| 8 | human | 23387 | SIK3 | SIK family kinase 3 | NM_025164.6 | 92% | 92% | (many diffs) |
| 9 | human | 23387 | SIK3 | SIK family kinase 3 | XM_011542722.1 | 91.1% | 91.2% | (many diffs) |
| 10 | human | 23387 | SIK3 | SIK family kinase 3 | NM_001281749.3 | 87.6% | 87.7% | (many diffs) |
| 11 | human | 23387 | SIK3 | SIK family kinase 3 | XM_005271484.3 | 87.6% | 87.6% | (many diffs) |
| 12 | human | 23387 | SIK3 | SIK family kinase 3 | XM_017017424.1 | 84.1% | 84.1% | (many diffs) |
| 13 | human | 23387 | SIK3 | SIK family kinase 3 | NM_001281748.3 | 83.8% | 83.8% | (many diffs) |
| 14 | human | 23387 | SIK3 | SIK family kinase 3 | XM_005271485.3 | 58.3% | 58.2% | (many diffs) |
| 15 | human | 23387 | SIK3 | SIK family kinase 3 | XM_017017427.1 | 54.7% | 54.6% | (many diffs) |
| 16 | human | 23387 | SIK3 | SIK family kinase 3 | XR_001747816.1 | 46.5% | (many diffs) | |
| 17 | human | 23387 | SIK3 | SIK family kinase 3 | XM_011542726.1 | 43.5% | 43.6% | (many diffs) |
| 18 | human | 23387 | SIK3 | SIK family kinase 3 | NM_001366687.1 | 11.5% | 11.1% | (many diffs) |
| 19 | mouse | 70661 | Sik3 | SIK family kinase 3 | NM_027498.3 | 84.8% | 87.8% | (many diffs) |
| 20 | mouse | 70661 | Sik3 | SIK family kinase 3 | XM_006510590.2 | 81.5% | 84.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 90
- ORF end:
- 4023
- ORF length:
- 3933
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaatccg 61 cggccgcccc cttcaccgaa ttcggaccca tgcccgcccg tatcggctac tacgagatcg 121 accgcaccat cggcaagggc aacttcgcgg tggtcaagcg ggccacgcac ctcgtcacca 181 aggccaaggt tgctatcaag atcatagata agacccagct ggatgaagaa aacttgaaga 241 agattttccg ggaagttcaa attatgaaga tgctttgcca cccccatatc atcaggctct 301 accaggttat ggagacagaa cggatgattt atctggtgac agaatatgct agtggagggg 361 aaatatttga ccacctggtg gcccatggta gaatggcaga aaaggaggca cgtcggaagt 421 tcaaacagat cgtcacagct gtctattttt gtcactgtcg gaacattgtt catcgtgatt 481 taaaagctga aaatttactt ctggatgcca atctgaatat caaaatagca gattttggtt 541 tcagtaacct cttcactcct gggcagctgc tgaagacctg gtgtggcagc cctccctatg 601 ctgcacctga actctttgaa ggaaaagaat atgatgggcc caaagtggac atctggagcc 661 ttggagttgt cctctacgtg cttgtgtgcg gtgccctgcc atttgatgga agcacactgc 721 agaatctgcg ggcccgcgtg ctgagtggaa agttccgcat cccatttttt atgtccacag 781 aatgtgagca tttgatccgc catatgttgg tgttagatcc caataagcgc ctctccatgg 841 agcagatctg caagcacaag tggatgaagc taggggacgc cgatcccaac tttgacaggt 901 taatagctga atgccaacaa ctaaaggaag aaagacaggt ggaccccctg aatgaggatg 961 tcctcttggc catggaggac atgggactgg acaaagaaca gacactgcag tcattaagat 1021 cagatgccta tgatcactat agtgcaatct acagcctgct gtgtgatcga cataagagac 1081 ataaaaccct gcgtctcgga gcacttccta gcatgccccg agccctggcc tttcaagcac 1141 cagtcaatat ccaggcggag caggcaggta ctgctatgaa catcagcgtt ccccaggtgc 1201 agctgatcaa cccagagaac caaattgtgg agccggatgg gacactgaat ttggacagtg 1261 atgagggtga agagccttcc cctgaagcat tggtgcgcta tttgtcaatg aggaggcaca 1321 cagtgggtgt ggctgaccca cgcacggaag ttatggaaga tctgcagaag ctcctacctg 1381 gctttcctgg agtcaacccc caggctccat tcctgcaggt ggcccctaat gtgaacttca 1441 tgcacaacct gttgcctatg caaaacttgc aaccaaccgg gcaacttgag tacaaggagc 1501 agtctctcct acagccgccc acgctacagc tgttgaatgg aatgggcccc cttggccgga 1561 gggcatcaga tggaggagcc aacatccaac tgcatgccca gcagctgctg aagcgcccac 1621 ggggaccctc tccgcttgtc accatgacac cagcagtgcc agcagttacc cctgtggacg 1681 aggagagctc agacggggag ccagaccagg aagctgtgca gaggtacttg gcaaataggt 1741 ccaaaagaca tacactggcc atgaccaacc ctacagctga gatcccaccg gacctacaac 1801 ggcagctagg acagcagcct ttccgttccc gggtctggcc tcctcacctg gtacctgatc 1861 agcatcgctc tacctacaag gactccaaca ctctgcacct ccctacggag cgtttctccc 1921 ctgtgcgccg gttctcagat ggggctgcga gcatccaggc cttcaaagct cacctggaaa 1981 aaatgggcaa caacagcagc atcaaacagc tgcagcagga gtgtgagcag ctgcagaaga 2041 tgtacggggg gcagattgat gaaagaaccc tggagaagac ccagcagcag catatgttat 2101 accagcagga gcagcaccat caaattctcc agcaacaaat tcaagactct atctgtcctc 2161 ctcagccatc tccacctctt caggctgcat gtgaaaatca gccagccctc cttacccatc 2221 agctccagag gttaaggatt cagccttcaa gcccaccccc caaccacccc aacaaccatc 2281 tcttcaggca gcccagtaat agtcctcccc ccatgagcag tgccatgatc cagcctcacg 2341 gggctgcatc ttcttcccag tttcaaggct taccttcccg cagtgcaatc tttcagcagc 2401 aacctgagaa ctgttcctct cctcccaacg tggcactaac ctgcttgggt atgcagcagc 2461 ctgctcagtc acagcaggtc accatccaag tccaagagcc tgttgacatg ctcagcaaca 2521 tgccaggcac agctgcaggc tccagtgggc gcggcatctc catcagcccc agtgctggtc 2581 agatgcagat gcagcaccgt accaacctga tggccaccct cagctatggg caccgtccct 2641 tgtccaagca gctgagtgct gacagtgcag aggctcacag cttgaacgtg aatcggttct 2701 cccctgctaa ctacgaccag gcgcatttac acccccatct gttttcggac cagtcccggg 2761 gttcccccag cagctacagc ccttcaacag gagtggggtt ctctccaacc caagccctga 2821 aagtccctcc acttgaccaa ttccccacct tccctcccag tgcacatcag cagccgccac 2881 actataccac gtcggcacta cagcaggccc tgctgtctcc cacgccgcca gactatacaa 2941 gacaccagca ggtaccccac atccttcaag gactgctttc tccccggcat tcgctcaccg 3001 gccactcgga catccggctg cccccaacag agtttgcaca gctcattaaa aggcagcagc 3061 aacaacggca gcagcagcag caacagcagc aacagcaaga ataccaggaa ctgttcaggc 3121 acatgaacca aggggatgcg gggagtctgg ctcccagcct tgggggacag agcatgacag 3181 agcgccaggc tttatcttat caaaatgctg actcttatca ccatcacacc agcccccagc 3241 atctgctaca aatcagggca caagaatgtg tctcacaggc ttcctcaccc accccgcccc 3301 acgggtatgc tcaccagccg gcactgatgc attcagagag catggaggag gactgctcgt 3361 gtgagggggc caaggatggc ttccaagaca gtaagagttc aagtacattg accaaaggtt 3421 gccatgacag ccctctgctc ttgagtaccg gtggacctgg ggaccgtgaa tctttgctag 3481 gaactgtgag tcatgcccaa gaattgggga tacatcccta tggtcatcag ccaactgctg 3541 cattcagtaa aaataaggtg cccagcagag agcctgtcat agggaactgc atggatagaa 3601 gttctccagg acaagcagtg gagctgccgg atcacaatgg gctcgggtac ccagcacgcc 3661 cctccgtcca tgagcaccac aggccccggg CCCTCCAGAG ACACCACACG ATCCAGAACA 3721 GCGACGATGC TTATGTACAG CTGGATAACT TGCCAGGAAT GAGTCTCGTG GCTGGGAAAG 3781 CACTTAGCTC TGCCCGGATG TCGGATGCAG TTCTCAATCA GTCTTCGCTC ATGGGCAGCC 3841 AGCAGTTTCA GGATGGGGAA AATGAGGAAT GTGGGGCAAG CCTGGGAGGT CATGAGCACC 3901 CAGACCTGAG TGATGGCAGC CAGCATTTAA ACTCCTCTTG CTATCCATCT ACGTGTATTA 3961 CAGACATTCT GCTCAGCTAC AAGCACCCCG AAGTCTCCTT CAGCATGGAG CAGGCAGGCG 4021 TGTAAGCTTA AGGGTGGGCG CGCCGACCCA GCTTTCTTGT ACAAAGTGGT TGATATCGGT 4081 AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA 4141 CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TGCGAAAACA 4201 AGTACTTATG AAACACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa 4261 gatt