Construct: ORF TRCN0000491254
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018891.3_s317c1
- Derived from:
- ccsbBroadEn_15013
- DNA Barcode:
- CTCAGTAGTCTGCAGGTTTCGGTT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BRD4 (23476)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491254
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23476 | BRD4 | bromodomain containing 4 | NM_014299.2 | 99.7% | 36.2% | (many diffs) |
| 2 | human | 23476 | BRD4 | bromodomain containing 4 | XM_011527856.3 | 99.7% | 36.2% | (many diffs) |
| 3 | human | 23476 | BRD4 | bromodomain containing 4 | NM_001330384.2 | 90.5% | 32.9% | (many diffs) |
| 4 | human | 23476 | BRD4 | bromodomain containing 4 | NM_058243.2 | 52.7% | 19.2% | (many diffs) |
| 5 | human | 23476 | BRD4 | bromodomain containing 4 | XM_011527854.2 | 52.7% | 19.2% | (many diffs) |
| 6 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_198094.2 | 89.5% | 34.9% | (many diffs) |
| 7 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524695.3 | 89.3% | 34.8% | (many diffs) |
| 8 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524691.1 | 47.2% | 18.5% | (many diffs) |
| 9 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524690.2 | 47.2% | 18.5% | (many diffs) |
| 10 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_020508.4 | 46.1% | 18.1% | (many diffs) |
| 11 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524688.1 | 46.1% | 18.1% | (many diffs) |
| 12 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524689.2 | 46.1% | 18.1% | (many diffs) |
| 13 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_001286630.1 | 46.1% | 18.1% | (many diffs) |
| 14 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524683.2 | 46.1% | 18.1% | (many diffs) |
| 15 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524684.2 | 46.1% | 18.1% | (many diffs) |
| 16 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524685.1 | 46.1% | 18.1% | (many diffs) |
| 17 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524686.1 | 46.1% | 18.1% | (many diffs) |
| 18 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524687.2 | 46.1% | 18.1% | (many diffs) |
| 19 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524693.2 | 36% | 7.6% | (many diffs) |
| 20 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_011246543.2 | 36% | 7.6% | (many diffs) |
| 21 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_017317588.1 | 36% | 7.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 969
- ORF length:
- 900
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctgcggag agcggccctg ggacgagatt gagaaatctg ccagtaatgg 121 gggatggact agaaacttcc caaatgtcta caacacaggc ccaggcccaa ccccagccag 181 ccaacgcagc cagcaccaac cccccgcccc cagagacctc caaccctaac aagcccaaga 241 ggcagaccaa ccaactgcaa tacctgctca gagtggtgct caagacacta tggaaacacc 301 agtttgcatg gcctttccag cagcctgtgg atgccgtcaa gctgaacctc cctgattact 361 ataagatcat taaaacgcct atggatatgg gaacaataaa gaagcgcttg gaaaacaact 421 attactggaa tgctcaggaa tgtatccagg acttcaacac tatgtttaca aattgttaca 481 tctacaacaa gcctggagat gacatagtct taatggcaga agctctggaa aagctcttct 541 tgcaaaaaat aaatgagcta cccacagaag aaaccgagat catgatagtc caggcaaaag 601 gaagaggacg tgggaggaaa gaaacaggga cagcaaaacc tggcgtttcc acggtaccaa 661 acacaactca agcatcgact cctccgcaga cccagacccc tcagccgaat cctcctcctg 721 tgcaggccac gcctcacccc ttccctgccg tcaccccgga cctcatcgtc cagacccctg 781 tcatgacagt ggtgcctccc cagccactgc agacgccccc cgccagtgcc cccccagcca 841 caaccccaac ccgcttcagc tccccagccc gtacagagcc acccacccat catcgcggcc 901 accccacagc ctgtgaagac aaagaaggga gtgaagagga aagcagacac caccaccccc 961 accaccattg accccattca cgagccaccc tcgctgcccc cggagcccaa gaccaccaag 1021 ctgggccagc ggcgggagag cagccggcct gtgaaacctc caaagaagga cgtgcccgac 1081 tctcagcagc acccagcacc agagaagagc agcaaggtct cggagcagct caagtgctgc 1141 agcggcatcc tcaaggagat gtttgccaag aagcacgccg cctacgcctg gcccttctac 1201 aagcctgtgg acgtggaggc actgggccta cacgactact gtgacatcat caagcacccc 1261 atggacatga gcacaatcaa gtctaaactg gaggcccgtg agtaccgtga tgctcaggag 1321 tttggtgctg acgtccgatt gatgttctcc aactgctata agtacaaccc tcctgaccat 1381 gaggtggtgg ccatggcccg caagctccag gatgtgttcg aaatgcgctt tgccaagatg 1441 ccggacgagc ctgaggagcc agtggtggcc gtgtcctccc ccggcagtgc tccctcccac 1501 caaggttgtg gccccgccct catcccagcg acagcagcag cgatagctcc tcggacagtg 1561 acagttcgac tgatgactct gaggaggagc gagcccagcg gctggctgag ctccaggagc 1621 agctcaaagc cgtgcacgag cagcttgcag ccctctctca gccccagcag aacaaaccaa 1681 agaaaaagga gaaagacaag aaggaaaaga aaaaagaaaa gcacaaaagg aaagaggaag 1741 tggaagagaa taaaaaaagc aaagccaagg aacctcctcc taaaaagacg aagaaaaata 1801 atagcagcaa cagcaatgtg agcaagaagg agccagcgcc catgaagagc aagccccctc 1861 ccacgtatga gtcggaggaa gaggacaagt gcaagcctat gtcctatgag gagaagcggc 1921 agctcagctt ggacatcaac aagctccccg gcgagaagct gggccgcgtg gtgcacatca 1981 tccagtcacg ggagcccTCC CTGAAGAATT CCAACCCCGA CGAGATTGAA ATCGACTTTG 2041 AGACCCTGAA GCCGTCCACA CTGCGTGAGC TGGAGCGCTA TGTCACCTCC TGTTTGCGGA 2101 AGAAAAGGAA ACCTCAAGCT GAGAAAGTTG ATGTGATTGC CGGCTCCTCC AAGATGAAGG 2161 GCTTCTCGTC CTCAGAGTCG GAGAGCTCCA GTGAGTCCAG CTCCTCTGAC AGCGAAGACT 2221 CCGAAACAGG TCCTGCCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 2281 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 2341 GTATTTCGAT TTCTTGGCTT ATATATCTTG TGGAAAGGAC GACTCAGTAG TCTGCAGGTT 2401 TCGGTTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga aagatt