Construct: ORF ccsbBroadEn_15013
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018891.1_s300c1, BRDN0000458187
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- BRD4 (23476)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23476 | BRD4 | bromodomain containing 4 | NM_014299.2 | 99.7% | 36.2% | (many diffs) |
| 2 | human | 23476 | BRD4 | bromodomain containing 4 | XM_011527856.3 | 99.7% | 36.2% | (many diffs) |
| 3 | human | 23476 | BRD4 | bromodomain containing 4 | NM_001330384.2 | 90.5% | 32.9% | (many diffs) |
| 4 | human | 23476 | BRD4 | bromodomain containing 4 | NM_058243.2 | 52.7% | 19.2% | (many diffs) |
| 5 | human | 23476 | BRD4 | bromodomain containing 4 | XM_011527854.2 | 52.7% | 19.2% | (many diffs) |
| 6 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_198094.2 | 89.5% | 34.9% | (many diffs) |
| 7 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524695.3 | 89.3% | 34.8% | (many diffs) |
| 8 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524691.1 | 47.2% | 18.5% | (many diffs) |
| 9 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524690.2 | 47.2% | 18.5% | (many diffs) |
| 10 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_020508.4 | 46.1% | 18.1% | (many diffs) |
| 11 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524688.1 | 46.1% | 18.1% | (many diffs) |
| 12 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524689.2 | 46.1% | 18.1% | (many diffs) |
| 13 | mouse | 57261 | Brd4 | bromodomain containing 4 | NM_001286630.1 | 46.1% | 18.1% | (many diffs) |
| 14 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524683.2 | 46.1% | 18.1% | (many diffs) |
| 15 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524684.2 | 46.1% | 18.1% | (many diffs) |
| 16 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524685.1 | 46.1% | 18.1% | (many diffs) |
| 17 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524686.1 | 46.1% | 18.1% | (many diffs) |
| 18 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524687.2 | 46.1% | 18.1% | (many diffs) |
| 19 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_006524693.2 | 36% | 7.6% | (many diffs) |
| 20 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_011246543.2 | 36% | 7.6% | (many diffs) |
| 21 | mouse | 57261 | Brd4 | bromodomain containing 4 | XM_017317588.1 | 36% | 7.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 99
- ORF end:
- 999
- ORF length:
- 900
- Sequence:
-
1 AACGGATTTT ATTTTGACTG ATAGTGACCT GTTCGTTGCA ACAAATTGAT GAGCAATGCT 61 TTTTTATAAT GCCAACTTTG TACAAAAAAG TTGGCACCAT GTCTGCGGAG AGCGGCCCTG 121 GGACGAGATT GAGAAATCTG CCAGTAATGG GGGATGGACT AGAAACTTCC CAAATGTCTA 181 CAACACAGGC CCAGGCCCAA CCCCAGCCAG CCAACGCAGC CAGCACCAAC CCCCCGCCCC 241 CAGAGACCTC CAACCCTAAC AAGCCCAAGA GGCAGACCAA CCAACTGCAA TACCTGCTCA 301 GAGTGGTGCT CAAGACACTA TGGAAACACC AGTTTGCATG GCCTTTCCAG CAGCCTGTGG 361 ATGCCGTCAA GCTGAACCTC CCTGATTACT ATAAGATCAT TAAAACGCCT ATGGATATGG 421 GAACAATAAA GAAGCGCTTG GAAAACAACT ATTACTGGAA TGCTCAGGAA TGTATCCAGG 481 ACTTCAACAC TATGTTTACA AATTGTTACA TCTACAACAA GCCTGGAGAT GACATAGTCT 541 TAATGGCAGA AGCTCTGGAA AAGCTCTTCT TGCAAAAAAT AAATGAGCTA CCCACAGAAG 601 AAACCGAGAT CATGATAGTC CAGGCAAAAG GAAGAGGACG TGGGAGGAAA GAAACAGGGA 661 CAGCAAAACC TGGCGTTTCC ACGGTACCAA ACACAACTCA AGCATCGACT CCTCCGCAGA 721 CCCAGACCCC TCAGCCGAAT CCTCCTCCTG TGCAGGCCAC GCCTCACCCC TTCCCTGCCG 781 TCACCCCGGA CCTCATCGTC CAGACCCCTG TCATGACAGT GGTGCCTCCC CAGCCACTGC 841 AGACGCCCCC CGCCAGTGCC CCCCCAGCCA CAACCCCAAC CCGCTTCagc tccccagccc 901 gtacagagcc acccacccat catcgcggcc accccacagc ctgtgaagac aaagaaggga 961 gtgaagagga aagcagacac caccaccccc accaccattg accccattca cgagccaccc 1021 tcgctgcccc cggagcccaa gaccaccaag ctgggccagc ggcgggagag cagccggcct 1081 gtgaaacctc caaagaagga cgtgcccgac tctcagcagc acccagcacc agagaagagc 1141 agcaaggtct cggagcagct caagtgctgc agcggcatcc tcaaggagat gtttgccaag 1201 aagcacgccg cctacgcctg gcccttctac aagcctgtgg acgtggaggc actgggccta 1261 cacgactact gtgacatcat caagcacccc atggacatga gcacaatcaa gtctaaactg 1321 gaggcccgtg agtaccgtga tgctcaggag tttggtgctg acgtccgatt gatgttctcc 1381 aactgctata agtacaaccc tcctgaccat gaggtggtgg ccatggcccg caagctccag 1441 gatgtgttcg aaatgcgctt tgccaagatg ccggacgagc ctgaggagcc agtggtggcc 1501 gtGTCCTCCC CCGGCAGTGC TCCCTCCCAC CAAGGTTGTG GCCCCGCCCT CATCCCAGCG 1561 ACAGCAGCAG CGATAGCTCC TCGGACAGTG ACAGTTCGAC TGATGACTCT GAGGAGGAGC 1621 GAGCCCAGCG GCTGGCTGAG CTCCAGGAGC AGCTCAAAGC CGTGCACGAG CAGCTTGCAG 1681 CCCTCTCTCA GCCCCAGCAG AACAAACCAA AGAAAAAGGA GAAAGACAAG AAGGAAAAGA 1741 AAAAAGAAAA GCACAAAAGG AAAGAGGAAG TGGAAGAGAA TAAAAAAAGC AAAGCCAAGG 1801 AACCTCCTCC TAAAAAGACG AAGAAAAATA ATAGCAGCAA CAGCAATGTG AGCAAGAAGG 1861 AGCCAGCGCC CATGAAGAGC AAGCCCCCTC CCACGTATGA GTCGGAGGAA GAGGACAAGT 1921 GCAAGCCTAT GTCCTATGAG GAGAAGCGGC AGCTCAGCTT GGACATCAAC AAGCTCCCCG 1981 GCGAGAAGCT GGGCCGCGTG GTGCACATCA TCCAGTCACG GGAGCCCTCC CTGAAGAATT 2041 CCAACCCCGA CGAGATTGAA ATCGACTTTG AGACCCTGAA GCCGTCCACA CTGCGTGAGC 2101 TGGAGCGCTA TGTCACCTCC TGTTTGCGGA AGAAAAGGAA ACCTCAAGCT GAGAAAGTTG 2161 ATGTGATTGC CGGCTCCTCC AAGATGAAGG GCTTCTCGTC CTCAGAGTCG GAGAGCTCCA 2221 GTGAGTCCAG CTCCTCTGAC AGCGAAGACT CCGAAACAGG TCCTGCCTTG CCAACTTTCT 2281 TGTACAAAGT TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA 2341 CTATCAGTCA AAATAAAATC ATTATTGCCA TCCCAGCCCG CAA