Construct: ORF TRCN0000491423

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006344.2_s317c1
Derived from:
ccsbBroadEn_02966
DNA Barcode:
CCGGTGATCCTGAAGAGTAGAGGC
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
DAZAP1 (26528)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000491423
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 26528 DAZAP1 DAZ associated protein 1 NM_018959.4 100% 100%
2 human 26528 DAZAP1 DAZ associated protein 1 NM_001352033.2 99.7% 99.7% 302_303insGCA
3 human 26528 DAZAP1 DAZ associated protein 1 NM_001352034.2 99.7% 99.7% 1047_1048insGCA
4 human 26528 DAZAP1 DAZ associated protein 1 XM_011527910.2 89.9% 89.9% 0_1ins123
5 human 26528 DAZAP1 DAZ associated protein 1 NM_170711.3 88.2% 84.9% (many diffs)
6 human 26528 DAZAP1 DAZ associated protein 1 XM_011527904.2 73.3% 71.8% (many diffs)
7 human 26528 DAZAP1 DAZ associated protein 1 XM_011527905.2 73.1% 71.6% (many diffs)
8 human 26528 DAZAP1 DAZ associated protein 1 XM_011527906.2 73.1% 71.6% (many diffs)
9 human 26528 DAZAP1 DAZ associated protein 1 XM_011527907.2 72.9% 71.4% (many diffs)
10 human 26528 DAZAP1 DAZ associated protein 1 XM_011527908.2 64.9% 61.2% (many diffs)
11 human 26528 DAZAP1 DAZ associated protein 1 XM_011527909.3 64.9% 61.2% (many diffs)
12 human 26528 DAZAP1 DAZ associated protein 1 NM_001352035.2 57.4% 55% 0_1ins436;27_28ins83
13 human 26528 DAZAP1 DAZ associated protein 1 XM_005259535.3 57.4% 55% 0_1ins436;27_28ins83
14 human 26528 DAZAP1 DAZ associated protein 1 XM_005259536.4 57.2% 54.8% 0_1ins436;27_28ins83;528_529insGCA
15 human 26528 DAZAP1 DAZ associated protein 1 XM_017026582.2 31.4% 26.2% (many diffs)
16 mouse 70248 Dazap1 DAZ associated protein 1 XM_006514069.3 89.1% 98.5% (many diffs)
17 mouse 70248 Dazap1 DAZ associated protein 1 NM_001122604.1 88.8% 98.2% (many diffs)
18 mouse 70248 Dazap1 DAZ associated protein 1 NM_001122605.1 88.8% 98.2% (many diffs)
19 mouse 70248 Dazap1 DAZ associated protein 1 NM_133188.2 88.6% 98% (many diffs)
20 mouse 70248 Dazap1 DAZ associated protein 1 XM_006514070.4 87.3% 96.8% (many diffs)
21 mouse 70248 Dazap1 DAZ associated protein 1 XM_006514071.4 87% 96.5% (many diffs)
22 mouse 70248 Dazap1 DAZ associated protein 1 XM_030245258.1 87% 96.5% (many diffs)
23 mouse 70248 Dazap1 DAZ associated protein 1 XM_030245259.1 86.8% 96.3% (many diffs)
24 mouse 70248 Dazap1 DAZ associated protein 1 XM_006514072.4 58% 64.1% (many diffs)
25 mouse 70248 Dazap1 DAZ associated protein 1 XM_006514073.4 57.8% 63.8% (many diffs)
26 mouse 70248 Dazap1 DAZ associated protein 1 XM_006514074.4 50.6% 54.5% (many diffs)
27 mouse 70248 Dazap1 DAZ associated protein 1 XM_011243559.3 50.6% 54.5% (many diffs)
28 mouse 70248 Dazap1 DAZ associated protein 1 XM_030245260.1 50.4% 54.2% (many diffs)
29 mouse 70248 Dazap1 DAZ associated protein 1 XM_030245261.1 50.4% 54.2% (many diffs)
30 mouse 70248 Dazap1 DAZ associated protein 1 XM_030245262.1 50.4% 54.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1287
ORF length:
1221
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgaa caactcgggc gccgacgaga tcgggaagct cttcgtgggc ggtcttgact
121ggagcacgac ccaagagact ctgcgcagct acttttccca atatggagaa gtcgtagatt
181gtgttatcat gaaagataaa accaccaacc agtctcgagg ctttgggttt gtcaaattta
241aagacccaaa ctgtgtgggg acggtgctgg ccagcagacc gcacacgcta gatggccgaa
301acatcgaccc caagccatgc acaccccggg ggatgcagcc ggagagaaca cggccgaagg
361aaggatggca gaaaggaccc aggagcgata acagtaaatc aaataagata tttgtcggtg
421gaattcctca caattgtggt gagacagagc tcagggaata cttcaagaag ttcggagtgg
481tcacggaggt agtcatgatc tatgacgccg agaagcagag gccccgaggt tttggattta
541ttactttcga ggacgaacaa tcagtggacc aggctgtcaa catgcatttt cacgacatca
601tgggcaaaaa agtggaagtt aaacgagctg agcctcggga cagcaagagc caagcgccgg
661gacagccagg tgccagccag tgggggagcc gggttgtgcc caacgctgcc aatggctggg
721caggccagcc cccgcccacg tggcagcaag gatatggccc gcaaggaatg tgggtgccgg
781caggacaggc gattggtggc tatggaccgc cccctgcagg aagaggagcc cccccgccac
841ccccaccgtt cacctcctac atcgtgtcca cccctcctgg aggctttccc cctccccagg
901gcttccctca gggctacggt gccccgccac agttcagttt tggctacggg cctccacctc
961caccgccaga tcagtttgcc cctccggggg ttcctcctcc accagccact cccggggcag
1021cacctctggc tttcccaccg ccTCCGTCTC AGGCTGCCCC GGACATGAGC AAGCCCCCGA
1081CAGCTCAGCC AGACTTCCCC TATGGTCAGT ATGCAGGTTA CGGGCAGGAC TTGAGTGGCT
1141TCGGACAGGG CTTCTCAGAC CCCAGCCAGC AGCCTCCTTC CTACGGGGGT CCCTCCGTGC
1201CAGGGTCGGG GGGCCCCCCC GCCGGCGGCA GCGGCTTTGG ACGAGGGCAG AACCACAACG
1261TGCAAGGGTT CCACCCCTAC CGACGCTACC CAACTTTCTT GTACAAAGTG GTTGATATCG
1321GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT
1381AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GACCGGTGAT
1441CCTGAAGAGT AGAGGCACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga
1501aagatt

Download FASTA (ORF) (Full)