Construct: ORF TRCN0000491644
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010112.1_s317c1
- Derived from:
- ccsbBroadEn_07831
- DNA Barcode:
- TAACATCATGGATAGATATGTGGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SAMD4A (23034)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000491644
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23034 | SAMD4A | sterile alpha motif domain ... | NM_001161576.2 | 99.7% | 99.8% | 1_3delATG;268C>T |
| 2 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449517.1 | 94.3% | 94.4% | 1_3delATG;268C>T;1779_1886del |
| 3 | human | 23034 | SAMD4A | sterile alpha motif domain ... | NM_015589.6 | 87.5% | 87.4% | 1_3delATG;268C>T;716_979del |
| 4 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449516.1 | 85.8% | 85.7% | (many diffs) |
| 5 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449514.1 | 83.3% | 83.2% | (many diffs) |
| 6 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449515.1 | 83.3% | 83.2% | (many diffs) |
| 7 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449519.1 | 79.5% | 79.5% | 0_1ins300;1476_1583del |
| 8 | human | 23034 | SAMD4A | sterile alpha motif domain ... | XM_024449518.1 | 70.2% | 70.1% | 0_1ins300;413_676del;1740_1847del |
| 9 | human | 23034 | SAMD4A | sterile alpha motif domain ... | NM_001161577.2 | 46.4% | 46.4% | 0_1ins960;816_923del |
| 10 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_028966.3 | 90.2% | 94.2% | (many diffs) |
| 11 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_001037221.2 | 79.1% | 82.6% | (many diffs) |
| 12 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | XM_006519627.2 | 74.1% | 77.2% | (many diffs) |
| 13 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_001163433.1 | 65.9% | 69% | (many diffs) |
| 14 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | XM_006519628.2 | 61.7% | 64.5% | (many diffs) |
| 15 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | NM_001310544.1 | 43.2% | 45% | (many diffs) |
| 16 | mouse | 74480 | Samd4 | sterile alpha motif domain ... | XM_006519631.3 | 43.2% | 45% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1956
- ORF length:
- 1887
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtttcgcgac caggtcgggg tgctggcggg ctggtttaag ggctggaacg 121 agtgcgagca gactgttgcg ctgctgtcgc tgctcaagcg cgtgagccag acccaggccc 181 gcttcctcca gctctgcctg gagcactcgc tggccgactg cgccgagctg cacgtcctcg 241 aacgcgaggc caacagcccc ggaatcatta accaatggca acaggaatcc aaggataaag 301 tgatttccct cctgttaact catctgcctt tgttgaagcc aggaaacctc gacgcgaaag 361 tagaatatat gaaactgctg cccaaaatcc tggctcactc tattgaacac aaccagcaca 421 ttgaggagag caggcagctg ctgtcctatg ctttgataca tccagccact tcgttagaag 481 accgtagtgc tttagccatg tggctgaatc acttggagga ccgcacgtcg accagctttg 541 gtggccagaa ccgaggccgc tcagactctg tggattatgg acagacacac tactatcacc 601 aaagacagaa ctctgatgac aagctcaatg ggtggcagaa ctctcgggat tctgggattt 661 gcatcaatgc ctccaactgg caggacaaaa gcatggggtg tgagaatggc catgtgcccc 721 tctactcctc ctcatctgtc cccaccacaa tcaatacgat tggaaccagc acaagtacaa 781 atgttccagc ctggctgaaa agcctccgcc tgcacaaata tgccgcgctt ttctcccaga 841 tgacctatga ggagatgatg gccctcaccg agtgccagct ggaggcgcag aatgttacca 901 aaggtgcaag acacaaaatt gtcatcagta ttcagaagct caaagaaaga caaaatctcc 961 tgaagtcttt ggaaagggac atcatcgagg ggggcagcct gcgcatcccg ctccaggaac 1021 tgcaccagat gatcctgact ccgatcaagg cctacagctc cccgagcacc acccccgagg 1081 ctcgccgccg ggagccccag gccccgcgtc agccctcact gatgggcccc gagagccaga 1141 gccccgactg caaagatggg gccgcagcca ctggcgccac ggccaccccc tcggccgggg 1201 ccagcggggg gctccagccg caccagctga gcagctgcga tggggagctg gccgtcgccc 1261 ccctgccaga gggggacctc cccgggcagt tcacacgcgt catggggaaa gtgtgcacac 1321 agctcttggt ctccagacct gatgaggaaa atataagttc ctatttacag ctcatagaca 1381 agtgtctaat tcatgaggca tttacagaga cacagaaaaa aagattgttg tcatggaaac 1441 agcaggtgca gaagctcttt cggtctttcc ctcggaaaac ccttctagac atatcaggat 1501 atcgacagca aagaaatcga ggctttgggc aatccaactc cctcccgacg gctggctctg 1561 tgggcggtgg catgggcaga cggaacccgc gccagtacca gatcccctct cGGAACGTCC 1621 CTTCCGCCCG CCTGGGCCTC TTGGGCACCA GTGGATTCGT CAGCTCCAAC CAGCGCAACA 1681 CCACAGCTAC CCCCACCATC ATGAAACAAG GAAGACAGAA CCTGTGGTTT GCCAACCCCG 1741 GGGGCAGCAA TAGCATGCCA AGCCGCACCC ACAGCTCAGT CCAGAGGACC CGCTCGCTGC 1801 CCGTGCACAC TTCCCCACAG AACATGCTGA TGTTCCAGCA GCCAGAATTC CAGCTTCCCG 1861 TGACCGAACC TGACATCAAC AACAGGCTGG AGTCGTTGTG CCTCAGTATG ACCGAACACG 1921 CCCTGGGAGA CGGGGTTGAC CGGACCTCCA CCATCTTGCC AACTTTCTTG TACAAAGTGG 1981 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 2041 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 2101 ATAACATCAT GGATAGATAT GTGGTACGCG TTAAGTCgac aatcaacctc tggattacaa 2161 aatttgtgaa agatt