Construct: ORF ccsbBroad304_02202

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF012139.1_s304c1, BRDN0000399910
Derived from:
ccsbBroadEn_02202
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
BABAM2 (9577)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02202
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9577 BABAM2 BRISC and BRCA1 A complex m... NM_001329112.1 100% 100%
2 human 9577 BABAM2 BRISC and BRCA1 A complex m... NM_199191.2 100% 100%
3 human 9577 BABAM2 BRISC and BRCA1 A complex m... NM_199194.2 100% 100%
4 human 9577 BABAM2 BRISC and BRCA1 A complex m... NM_001261840.2 96.2% 94.7% 1088_1089ins28;1122_1137del
5 human 9577 BABAM2 BRISC and BRCA1 A complex m... NM_199192.2 95.2% 95% (many diffs)
6 human 9577 BABAM2 BRISC and BRCA1 A complex m... NM_199193.2 95.2% 95% (many diffs)
7 human 9577 BABAM2 BRISC and BRCA1 A complex m... NM_001329113.1 90.3% 88.9% (many diffs)
8 human 9577 BABAM2 BRISC and BRCA1 A complex m... NM_004899.4 90.3% 88.9% (many diffs)
9 human 9577 BABAM2 BRISC and BRCA1 A complex m... NM_001329115.1 87% 86.8% 1089_1259del
10 human 9577 BABAM2 BRISC and BRCA1 A complex m... NM_001329114.1 78.2% 74.2% (many diffs)
11 human 9577 BABAM2 BRISC and BRCA1 A complex m... NR_137437.1 59.4% 1_252del;1103_1104ins83;1319_1709del
12 human 9577 BABAM2 BRISC and BRCA1 A complex m... NR_137439.1 56.3% 1_252del;1341_1586del;1648_2038del
13 human 9577 BABAM2 BRISC and BRCA1 A complex m... NR_137438.1 55.2% 1_252del;1341_1628del;1690_2080del
14 human 9577 BABAM2 BRISC and BRCA1 A complex m... NR_137440.1 55.1% 1_252del;1341_1632del;1694_2084del
15 mouse 107976 Babam2 BRISC and BRCA1 A complex m... NM_144541.1 90.2% 99.2% (many diffs)
16 mouse 107976 Babam2 BRISC and BRCA1 A complex m... NM_181280.1 84.1% 85% (many diffs)
17 mouse 107976 Babam2 BRISC and BRCA1 A complex m... NM_181279.1 81.5% 87.3% (many diffs)
18 mouse 107976 Babam2 BRISC and BRCA1 A complex m... XM_011240687.3 75.6% 82.5% (many diffs)
19 mouse 107976 Babam2 BRISC and BRCA1 A complex m... NM_181281.1 75% 81.4% (many diffs)
20 mouse 107976 Babam2 BRISC and BRCA1 A complex m... NM_181282.1 59.1% 63.9% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1215
ORF length:
1149
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc cccagaagtg gccttgaacc gaatatctcc aatgctctcc cctttcatat
121ctagcgtggt ccggaatgga aaagtgggac tggatgctac aaactgtttg aggataactg
181acttaaaatc tggctgcaca tcattgactc ctgggcccaa ctgtgaccga tttaaactgc
241acataccata tgctggagag acattaaagt gggatatcat tttcaatgcc caatacccag
301aactgcctcc cgattttatc tttggagaag atgctgaatt cctgccagac ccctcagctt
361tgcagaatct tgcctcctgg aatccttcaa atcctgaatg tctcttactt gtggtgaagg
421aacttgtgca acaatatcac caattccaat gtagccgcct ccgggagagc tcccgcctca
481tgtttgaata ccagacatta ctggaggagc cacagtatgg agagaacatg gaaatttatg
541ctgggaaaaa aaacaactgg actggtgaat tttcagctcg tttccttttg aagctgcccg
601tagatttcag caatatcccc acataccttc tcaaggatgt aaatgaagac cctggagaag
661atgtggccct cctctctgtt agttttgagg acactgaagc cacccaggtg taccccaagc
721tgtacttgtc acctcgaatt gagcatgcac ttggaggctc ctcagctctt catatcccag
781cttttccagg aggaggatgt ctcattgatt acgttcctca agtatgccac ctgctcacca
841acaaggtgca gtacgtgatt caagggtatc acaaaagaag agagtatatt gctgcttttc
901tcagtcactt tggcacaggt gtcgtggaat atgatgcaga aggctttaca aaactcactc
961tgctgctgat gtggaaagat ttttgttttc ttgtacacat tgacctgcct ctgtttttcc
1021ctcgagacca gccaactctc acatttcagt ccgtttatca ctttaccaac agtggacagc
1081tttactccca ggcccaaaaa aattatccgt acagccccag atgggatgga aatgaaatgg
1141ccaaaagagc aaaggcttat ttcaaaacct ttgtccctca gttccaggag gcagcatttg
1201ccaatggaaa gctctacncc aactttcttg tacaaagtgg ttggtaagcc tatccctaac
1261cctctcctcg gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag
1321tcgacaatca acctctggat ta

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