Construct: ORF ccsbBroad304_02208
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014747.1_s304c1, BRDN0000405703
- Derived from:
- ccsbBroadEn_02208
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PDIA4 (9601)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_02208
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9601 | PDIA4 | protein disulfide isomerase... | NM_004911.5 | 100% | 100% | |
| 2 | human | 9601 | PDIA4 | protein disulfide isomerase... | NM_001371244.1 | 99.8% | 99.8% | 1129_1131delCAG |
| 3 | human | 9601 | PDIA4 | protein disulfide isomerase... | NM_001371245.1 | 95% | 90.9% | 614_627del;750_751ins83 |
| 4 | human | 9601 | PDIA4 | protein disulfide isomerase... | NR_163909.1 | 70.1% | (many diffs) | |
| 5 | human | 9601 | PDIA4 | protein disulfide isomerase... | NR_163908.1 | 69.9% | 1_82del;696_700delGGTAA;2023_2768del | |
| 6 | human | 9601 | PDIA4 | protein disulfide isomerase... | NR_163907.1 | 63.6% | 1_97del;1134_1135ins166;1867_2612del | |
| 7 | human | 9601 | PDIA4 | protein disulfide isomerase... | NR_163906.1 | 63.1% | 1_97del;184_185ins181;1852_2597del | |
| 8 | human | 9601 | PDIA4 | protein disulfide isomerase... | NR_163905.1 | 62% | 1_97del;185_522del;2371_3116del | |
| 9 | mouse | 12304 | Pdia4 | protein disulfide isomerase... | NM_009787.2 | 86.3% | 88.5% | (many diffs) |
| 10 | mouse | 12304 | Pdia4 | protein disulfide isomerase... | XM_006505450.3 | 62.5% | 61% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2001
- ORF length:
- 1935
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag gccccggaaa gccttcctgc tcctgctgct cttggggctg gtgcagctgc 121 tggccgtggc gggtgccgag ggcccggacg aggattcttc taacagagaa aatgccattg 181 aggatgaaga ggaggaggag gaggaagatg atgatgagga agaagacgac ttggaagtta 241 aggaagaaaa tggagtcttg gtcctaaatg atgcaaactt tgataatttt gtggctgaca 301 aagacacagt gctgctggag ttttatgctc catggtgtgg acattgcaag cagtttgctc 361 cggaatatga aaaaattgcc aacatattaa aggataaaga tcctcccatt cctgttgcca 421 agatcgatgc aacctcagcg tctgtgctgg ccagcaggtt tgatgtgagt ggctacccca 481 ccatcaagat ccttaagaag gggcaggctg tagactacga gggctccaga acccaggaag 541 aaattgttgc caaggtcaga gaagtctccc agcccgactg gacgcctcca ccagaagtca 601 cgcttgtgtt gaccaaagag aactttgatg aagttgtgaa tgatgcagat atcattctgg 661 tggagtttta tgccccatgg tgtggacact gcaagaaact tgcccccgag tatgagaagg 721 ccgccaagga gctcagcaag cgttctcctc caattcccct ggcaaaggtc gacgccaccg 781 cagaaacaga cctggccaag aggtttgatg tctctggcta tcccaccctg aaaattttcc 841 gcaaaggaag gccttatgac tacaacggcc cacgagaaaa atatggaatc gttgattaca 901 tgatcgagca gtccgggcct ccctccaagg agattctgac cctgaagcag gtccaggagt 961 tcctgaagga tggagacgat gtcatcatca tcggggtctt taagggggag agtgacccag 1021 cctaccagca ataccaggat gccgctaaca acctgagaga agattacaaa tttcaccaca 1081 ctttcagcac agaaatagca aagttcttga aagtctccca ggggcagttg gttgtaatgc 1141 agcctgagaa attccagtcc aagtatgagc cccggagcca catgatggac gtccagggct 1201 ccacccagga ctcggccatc aaggacttcg tgctgaagta cgccctgccc ctggttggcc 1261 accgcaaggt gtcaaacgat gctaagcgct acaccaggcg ccccctggtg gtcgtctact 1321 acagtgtgga cttcagcttt gattacagag ctgcaactca gttttggcgg agcaaagtcc 1381 tagaggtggc caaggacttc cctgagtaca cctttgccat tgcggacgaa gaggactatg 1441 ctggggaggt gaaggacctg gggctcagcg agagtgggga ggatgtcaat gccgccatcc 1501 tggacgagag tgggaagaag ttcgccatgg agccagagga gtttgactct gacaccctcc 1561 gcgagtttgt cactgctttc aaaaaaggaa aactgaagcc agtcatcaaa tcccagccag 1621 tgcccaagaa caacaaggga cccgtcaagg tcgtggtggg aaagaccttt gactccattg 1681 tgatggaccc caagaaggac gtcctcatcg agttctacgc gccatggtgc gggcactgca 1741 agcagctaga gcccgtgtac aacagcctgg ccaagaagta caagggccaa aagggcctgg 1801 tcatcgccaa gatggacgcc actgccaacg acgtccccag cgaccgctat aaggtggagg 1861 gcttccccac catctacttc gcccccagtg gggacaaaaa gaacccagtt aaatttgagg 1921 gtggagacag agatctggag catttgagca agtttataga agaacatgcc acaaaactga 1981 gcaggaccaa ggaagagctt tgcccaactt tcttgtacaa agtggttggt aagcctatcc 2041 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 2101 ttaagtcgac aatcaacctc tggatta