Construct: ORF ccsbBroad304_02208

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014747.1_s304c1, BRDN0000405703
Derived from:
ccsbBroadEn_02208
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PDIA4 (9601)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_02208
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 9601 PDIA4 protein disulfide isomerase... NM_004911.5 100% 100%
2 human 9601 PDIA4 protein disulfide isomerase... NM_001371244.1 99.8% 99.8% 1129_1131delCAG
3 human 9601 PDIA4 protein disulfide isomerase... NM_001371245.1 95% 90.9% 614_627del;750_751ins83
4 human 9601 PDIA4 protein disulfide isomerase... NR_163909.1 70.1% (many diffs)
5 human 9601 PDIA4 protein disulfide isomerase... NR_163908.1 69.9% 1_82del;696_700delGGTAA;2023_2768del
6 human 9601 PDIA4 protein disulfide isomerase... NR_163907.1 63.6% 1_97del;1134_1135ins166;1867_2612del
7 human 9601 PDIA4 protein disulfide isomerase... NR_163906.1 63.1% 1_97del;184_185ins181;1852_2597del
8 human 9601 PDIA4 protein disulfide isomerase... NR_163905.1 62% 1_97del;185_522del;2371_3116del
9 mouse 12304 Pdia4 protein disulfide isomerase... NM_009787.2 86.3% 88.5% (many diffs)
10 mouse 12304 Pdia4 protein disulfide isomerase... XM_006505450.3 62.5% 61% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2001
ORF length:
1935
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag gccccggaaa gccttcctgc tcctgctgct cttggggctg gtgcagctgc
121tggccgtggc gggtgccgag ggcccggacg aggattcttc taacagagaa aatgccattg
181aggatgaaga ggaggaggag gaggaagatg atgatgagga agaagacgac ttggaagtta
241aggaagaaaa tggagtcttg gtcctaaatg atgcaaactt tgataatttt gtggctgaca
301aagacacagt gctgctggag ttttatgctc catggtgtgg acattgcaag cagtttgctc
361cggaatatga aaaaattgcc aacatattaa aggataaaga tcctcccatt cctgttgcca
421agatcgatgc aacctcagcg tctgtgctgg ccagcaggtt tgatgtgagt ggctacccca
481ccatcaagat ccttaagaag gggcaggctg tagactacga gggctccaga acccaggaag
541aaattgttgc caaggtcaga gaagtctccc agcccgactg gacgcctcca ccagaagtca
601cgcttgtgtt gaccaaagag aactttgatg aagttgtgaa tgatgcagat atcattctgg
661tggagtttta tgccccatgg tgtggacact gcaagaaact tgcccccgag tatgagaagg
721ccgccaagga gctcagcaag cgttctcctc caattcccct ggcaaaggtc gacgccaccg
781cagaaacaga cctggccaag aggtttgatg tctctggcta tcccaccctg aaaattttcc
841gcaaaggaag gccttatgac tacaacggcc cacgagaaaa atatggaatc gttgattaca
901tgatcgagca gtccgggcct ccctccaagg agattctgac cctgaagcag gtccaggagt
961tcctgaagga tggagacgat gtcatcatca tcggggtctt taagggggag agtgacccag
1021cctaccagca ataccaggat gccgctaaca acctgagaga agattacaaa tttcaccaca
1081ctttcagcac agaaatagca aagttcttga aagtctccca ggggcagttg gttgtaatgc
1141agcctgagaa attccagtcc aagtatgagc cccggagcca catgatggac gtccagggct
1201ccacccagga ctcggccatc aaggacttcg tgctgaagta cgccctgccc ctggttggcc
1261accgcaaggt gtcaaacgat gctaagcgct acaccaggcg ccccctggtg gtcgtctact
1321acagtgtgga cttcagcttt gattacagag ctgcaactca gttttggcgg agcaaagtcc
1381tagaggtggc caaggacttc cctgagtaca cctttgccat tgcggacgaa gaggactatg
1441ctggggaggt gaaggacctg gggctcagcg agagtgggga ggatgtcaat gccgccatcc
1501tggacgagag tgggaagaag ttcgccatgg agccagagga gtttgactct gacaccctcc
1561gcgagtttgt cactgctttc aaaaaaggaa aactgaagcc agtcatcaaa tcccagccag
1621tgcccaagaa caacaaggga cccgtcaagg tcgtggtggg aaagaccttt gactccattg
1681tgatggaccc caagaaggac gtcctcatcg agttctacgc gccatggtgc gggcactgca
1741agcagctaga gcccgtgtac aacagcctgg ccaagaagta caagggccaa aagggcctgg
1801tcatcgccaa gatggacgcc actgccaacg acgtccccag cgaccgctat aaggtggagg
1861gcttccccac catctacttc gcccccagtg gggacaaaaa gaacccagtt aaatttgagg
1921gtggagacag agatctggag catttgagca agtttataga agaacatgcc acaaaactga
1981gcaggaccaa ggaagagctt tgcccaactt tcttgtacaa agtggttggt aagcctatcc
2041ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
2101ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)