Construct: ORF ccsbBroad304_03924
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015023.1_s304c1, BRDN0000406135
- Derived from:
- ccsbBroadEn_03924
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CENPK (64105)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_03924
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64105 | CENPK | centromere protein K | NM_001267038.2 | 100% | 100% | |
| 2 | human | 64105 | CENPK | centromere protein K | NM_022145.5 | 100% | 100% | |
| 3 | human | 64105 | CENPK | centromere protein K | XM_011543536.2 | 100% | 100% | |
| 4 | human | 64105 | CENPK | centromere protein K | XM_017009690.1 | 100% | 100% | |
| 5 | human | 64105 | CENPK | centromere protein K | XM_017009691.1 | 100% | 100% | |
| 6 | human | 64105 | CENPK | centromere protein K | XM_017009692.1 | 100% | 100% | |
| 7 | human | 64105 | CENPK | centromere protein K | NM_001349367.2 | 99% | 98.5% | 5_12delCCCTCTTCinsAT |
| 8 | human | 64105 | CENPK | centromere protein K | XM_011543535.2 | 99% | 98.5% | 5_12delCCCTCTTCinsAT |
| 9 | human | 64105 | CENPK | centromere protein K | XM_011543537.2 | 88.8% | 88.8% | 0_1ins90 |
| 10 | human | 64105 | CENPK | centromere protein K | XM_017009694.1 | 88.8% | 88.8% | 0_1ins90 |
| 11 | human | 64105 | CENPK | centromere protein K | XM_011543539.2 | 87.7% | 87.7% | 368_369ins99 |
| 12 | human | 64105 | CENPK | centromere protein K | XM_017009695.1 | 87.3% | 86.2% | (many diffs) |
| 13 | human | 64105 | CENPK | centromere protein K | XM_011543538.2 | 86.8% | 86.3% | 5_12delCCCTCTTCinsAT;374_375ins99 |
| 14 | human | 64105 | CENPK | centromere protein K | XM_017009693.1 | 85% | 80.8% | (many diffs) |
| 15 | human | 64105 | CENPK | centromere protein K | XM_024446144.1 | 81.4% | 80.4% | (many diffs) |
| 16 | human | 64105 | CENPK | centromere protein K | XM_017009696.2 | 80.9% | 80% | (many diffs) |
| 17 | human | 64105 | CENPK | centromere protein K | XM_011543540.2 | 80.6% | 79.1% | (many diffs) |
| 18 | human | 64105 | CENPK | centromere protein K | XM_017009697.1 | 77.3% | 77.3% | 0_1ins183 |
| 19 | human | 64105 | CENPK | centromere protein K | NM_001349368.2 | 76.5% | 76.5% | 0_1ins90;278_279ins99 |
| 20 | human | 64105 | CENPK | centromere protein K | XM_011543541.2 | 72.4% | 71.3% | (many diffs) |
| 21 | human | 64105 | CENPK | centromere protein K | XM_011543542.2 | 72.4% | 71.3% | (many diffs) |
| 22 | human | 64105 | CENPK | centromere protein K | XM_024446145.1 | 72.4% | 71.3% | (many diffs) |
| 23 | human | 64105 | CENPK | centromere protein K | XM_024446146.1 | 70.8% | 69.8% | (many diffs) |
| 24 | human | 64105 | CENPK | centromere protein K | XM_024446147.1 | 70.8% | 69.8% | (many diffs) |
| 25 | human | 64105 | CENPK | centromere protein K | NM_001349369.2 | 70.3% | 69.3% | (many diffs) |
| 26 | human | 64105 | CENPK | centromere protein K | XM_017009701.1 | 60.9% | 60.9% | 0_1ins315 |
| 27 | human | 64105 | CENPK | centromere protein K | XM_024446148.1 | 60.3% | 59.1% | (many diffs) |
| 28 | human | 64105 | CENPK | centromere protein K | XR_001742177.1 | 60.3% | (many diffs) | |
| 29 | human | 64105 | CENPK | centromere protein K | XR_948282.3 | 32.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 873
- ORF length:
- 807
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa tcaggaggat ctagatccgg atagtactac agatgtggga gatgttacaa 121 atactgaaga agaacttatt agagaatgtg aagaaatgtg gaaagatatg gaagaatgtc 181 agaataaatt atcacttatt ggaactgaaa cactcaccga ttcaaatgct cagctatcat 241 tgttaattat gcaagtaaaa tgtttaaccg ctgaactcag tcaatggcag aaaaaaacac 301 ctgaaacaat tcccttgact gaagacgttc tcataacatt aggaaaagaa gagttccaaa 361 agctgagaca agatcttgaa atggtactgt ccactaagga gtcaaagaat gaaaagttaa 421 aggaagactt agaaagggaa caacggtggt tggatgaaca gcaacagata atggaatctc 481 ttaatgtact acacagtgaa ttgaaaaata aggttgaaac attttctgaa tcaagaatct 541 ttaatgaact gaaaactaaa atgcttaata taaaagaata taaggagaaa ctcttgagta 601 ccttgggcga gtttctagaa gaccattttc ctctgcctga tagaagtgtt aaaaagaaaa 661 agaaaaacat tcaagaatca tctgtaaacc tgataacact gcatgaaatg ttagagattc 721 ttataaatag attatttgat gttccacatg atccatatgt caaaattagt gattcctttt 781 ggccacctta tgttgagctg ctgctgcgta atggaattgc cttgagacat ccagaagatc 841 caacccgaat aagattagaa gctttccatc agtacccaac tttcttgtac aaagtggttg 901 gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga gctagcgcta 961 accggtggcg cgttaagtcg acaatcaacc tctggatta