Construct: ORF ccsbBroad304_07591

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000413.1_s304c1, BRDN0000399040
Derived from:
ccsbBroadEn_07591
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TUBB4A (10382)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_07591
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289129.1 99.9% 100% 774T>C
2 human 10382 TUBB4A tubulin beta 4A class IVa NM_006087.4 99.9% 100% 774T>C
3 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289127.1 90.7% 90.7% 1_135del;909T>C
4 human 7280 TUBB2A tubulin beta 2A class IIa NM_001069.3 90.2% 95% (many diffs)
5 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289123.1 89.6% 89.6% 1_153del;927T>C
6 human 347733 TUBB2B tubulin beta 2B class IIb NM_178012.5 89.4% 95.5% (many diffs)
7 human 10381 TUBB3 tubulin beta 3 class III NM_006086.4 87.8% 90.8% (many diffs)
8 human 10383 TUBB4B tubulin beta 4B class IVb NM_006088.6 87.3% 98.4% (many diffs)
9 human 84617 TUBB6 tubulin beta 6 class V NM_001303524.1 86.3% 91.7% (many diffs)
10 human 84617 TUBB6 tubulin beta 6 class V NM_032525.3 86.3% 91.7% (many diffs)
11 human 203068 TUBB tubulin beta class I NM_178014.4 84% 96.4% (many diffs)
12 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289130.1 83.7% 83.7% 0_1ins216;558T>C
13 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289131.1 83.7% 83.7% 0_1ins216;558T>C
14 human 347688 TUBB8 tubulin beta 8 class VIII NM_177987.2 82.6% 89.8% (many diffs)
15 human 260334 TUBB8B tubulin beta 8B NM_001358689.1 81.9% 88% (many diffs)
16 human 84617 TUBB6 tubulin beta 6 class V NM_001303526.2 80.8% 84.5% (many diffs)
17 human 203068 TUBB tubulin beta class I NM_001293212.2 79.7% 89.4% (many diffs)
18 human 203068 TUBB tubulin beta class I NM_001293214.2 76% 86.9% (many diffs)
19 human 10381 TUBB3 tubulin beta 3 class III NM_001197181.2 75.1% 77.1% (many diffs)
20 human 84617 TUBB6 tubulin beta 6 class V NM_001303528.2 74.7% 72.6% (many diffs)
21 human 84617 TUBB6 tubulin beta 6 class V NM_001303527.2 74.2% 78.2% (many diffs)
22 human 7280 TUBB2A tubulin beta 2A class IIa NM_001310315.2 74.1% 75.6% (many diffs)
23 human 203068 TUBB tubulin beta class I NM_001293215.2 70.7% 81.3% (many diffs)
24 human 203068 TUBB tubulin beta class I NM_001293216.2 70.7% 81.3% (many diffs)
25 human 347688 TUBB8 tubulin beta 8 class VIII XM_011519459.3 69.5% 75.6% (many diffs)
26 human 260334 TUBB8B tubulin beta 8B XM_024451145.1 69% 74.5% (many diffs)
27 human 260334 TUBB8B tubulin beta 8B XM_024451146.1 67.8% 71.8% (many diffs)
28 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016192.2 62.2% 67.3% (many diffs)
29 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016193.2 62.2% 67.3% (many diffs)
30 human 260334 TUBB8B tubulin beta 8B XM_024451147.1 61.5% 66.6% (many diffs)
31 human 84617 TUBB6 tubulin beta 6 class V NM_001303529.2 60.7% 62.8% (many diffs)
32 human 84617 TUBB6 tubulin beta 6 class V NM_001303530.2 60.7% 62.8% (many diffs)
33 human 203068 TUBB tubulin beta class I NM_001293213.2 45.9% 51.4% (many diffs)
34 mouse 22153 Tubb4a tubulin, beta 4A class IVA NM_009451.3 87.8% 100% (many diffs)
35 mouse 73710 Tubb2b tubulin, beta 2B class IIB NM_023716.2 87.4% 95.5% (many diffs)
36 mouse 22151 Tubb2a tubulin, beta 2A class IIA NM_009450.2 86.1% 95% (many diffs)
37 mouse 227613 Tubb4b tubulin, beta 4B class IVB NM_146116.2 84.3% 98.4% (many diffs)
38 mouse 22154 Tubb5 tubulin, beta 5 class I NM_011655.5 83.9% 96.4% (many diffs)
39 mouse 67951 Tubb6 tubulin, beta 6 class V NM_026473.2 83.5% 90.3% (many diffs)
40 mouse 22152 Tubb3 tubulin, beta 3 class III NM_023279.2 83.5% 90.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1398
ORF length:
1332
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaaa
61ttggcatgcg ggagatcgtg cacctgcagg ccggccagtg cggcaaccag atcggggcca
121agttttggga ggttatcagt gacgaacatg gcatcgaccc cacaggcaca taccatgggg
181acagtgacct gcaactggag aggatcaacg tgtactacaa cgaggccaca ggaggaaatt
241atgtccccag agcggtgctg gtggacctgg aacccggcac catggactct gtccgttctg
301gccccttcgg tcagatcttt cggccggaca acttcgtgtt tggccaatcc ggagccggca
361acaactgggc aaaggggcac tacacggagg gcgcagagct ggtggacgct gtcctggacg
421tagtccggaa ggaggccgag agctgcgact gccttcaggg cttccagctg acccactcgc
481tggggggtgg cacggggtcc ggaatgggca cgctgctcat cagtaagatc cgcgaggagt
541tcccagaccg catcatgaac accttcagcg tggtgccctc gcccaaagtg tcagacacgg
601tggtggagcc ctacaacgcc acgctgtctg tgcaccagct ggtggagaat acggatgaga
661cctactgcat cgacaacgag gcactctacg acatctgttt ccgcaccctc aagctgacca
721cccccaccta cggggacctc aaccacctgg tgtcggccac catgagcggg gtcaccacct
781gcctgcgctt cccgggccag ctgaacgccg acctgcgcaa gctggccgtc aacatggtcc
841cctttcctcg cctgcacttc ttcatgcccg gcttcgcacc cctgaccagc cggggcagcc
901agcagtaccg ggccctgacg gtgcccgagc tcacccagca gatgttcgat gccaagaaca
961tgatggcggc gtgcgacccg cgccacggcc gctacctgac cgtggccgcc gtgttccggg
1021gccgcatgtc catgaaggag gtggacgagc agatgctgag cgtgcagagc aagaacagca
1081gctacttcgt ggagtggatc cccaacaacg tgaagacggc cgtgtgcgac atcccgcccc
1141gcggcctgaa gatggccgcg accttcatcg gcaacagcac ggccatccag gagctgttca
1201agcgcatctc cgagcagttc acggccatgt tccggcgcaa ggccttcttg cactggtaca
1261cgggcgaggg catggacgag atggagttca ccgaggccga gagcaacatg aatgacctgg
1321tatctgagta ccagcagtac caggacgcca cggccgagga gggcgagttc gaggaggagg
1381cggaggagga ggtggcctac ccaactttct tgtacaaagt ggttggtaag cctatcccta
1441accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
1501agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)