Construct: ORF ccsbBroad304_10968
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003521.1_s304c1, BRDN0000402011
- Derived from:
- ccsbBroadEn_10968
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIA2 (4253)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_10968
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001247990.2 | 100% | 100% | |
2 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354140.2 | 99.3% | 99.3% | 143_144insGGTAGAAAATCAAAT |
3 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354141.2 | 99.3% | 99.3% | 143_144insGGTAGAAAATCAAAT |
4 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_011536785.2 | 99.3% | 99.3% | 143_144insGGTAGAAAATCAAAT |
5 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_017021323.1 | 98.7% | 98.7% | 159_185del |
6 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_017021324.2 | 98.7% | 98.7% | 159_185del |
7 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449598.1 | 98.7% | 98.7% | 159_185del |
8 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354156.2 | 95.7% | 95.7% | 65_66ins93 |
9 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354142.1 | 93.4% | 93.4% | 143_144insGGTAGAAAATCAAAT;1299_1300ins129 |
10 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354143.1 | 93.4% | 93.4% | 143_144insGGTAGAAAATCAAAT;1299_1300ins129 |
11 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354144.2 | 93.4% | 93.4% | 143_144insGGTAGAAAATCAAAT;1299_1300ins129 |
12 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_017021327.1 | 93.4% | 93.4% | 143_144insGGTAGAAAATCAAAT;1299_1300ins129 |
13 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354137.1 | 92.8% | 92.8% | 1_153del;296_297insGGTAGAAAATCAAAT |
14 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354138.1 | 92.8% | 92.8% | 1_153del;296_297insGGTAGAAAATCAAAT |
15 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_203356.2 | 92.8% | 92.8% | 1_153del;296_297insGGTAGAAAATCAAAT |
16 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_017021317.2 | 92.3% | 92.3% | 1_153del;312_338del |
17 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_017021318.1 | 92.3% | 92.3% | 1_153del;312_338del |
18 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_017021319.1 | 92.3% | 92.3% | 1_153del;312_338del |
19 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_017021330.1 | 92.3% | 92.3% | 1_153del;312_338del |
20 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_011536778.2 | 90.9% | 90.6% | (many diffs) |
21 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_203354.3 | 90.8% | 90.8% | 1_204del;347_348insGGTAGAAAATCAAAT |
22 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_017021316.1 | 90.4% | 90.4% | 1_204del;363_389del |
23 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354150.2 | 90.1% | 90.1% | 1_222del;365_366insGGTAGAAAATCAAAT |
24 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001247989.1 | 90.1% | 90.1% | 1_240del |
25 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_017021315.2 | 89.7% | 89.7% | 1_222del;381_407del |
26 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354151.2 | 89.5% | 89.2% | (many diffs) |
27 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_005930.4 | 89.4% | 89.4% | 1_240del;383_384insGGTAGAAAATCAAAT |
28 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354147.1 | 89.4% | 89.4% | 1_153del;218_219ins93 |
29 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_017021314.1 | 89.1% | 89.1% | 1_240del;399_425del |
30 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354145.2 | 87.7% | 86.9% | (many diffs) |
31 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354148.1 | 87.5% | 86.7% | (many diffs) |
32 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001247988.1 | 87.3% | 87.3% | 1_153del;296_297insGGTAGAAAATCAAAT;1452_1453ins129 |
33 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354139.1 | 87.3% | 87.3% | 1_153del;296_297insGGTAGAAAATCAAAT;1452_1453ins129 |
34 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354157.2 | 86.2% | 86.2% | 1_240del;305_306ins93 |
35 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354146.2 | 85.4% | 85.4% | 1_204del;347_348insGGTAGAAAATCAAAT;1503_1504ins129 |
36 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354154.2 | 84.3% | 83.6% | (many diffs) |
37 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354152.2 | 84.2% | 84% | (many diffs) |
38 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_203355.3 | 84.1% | 84.1% | 1_240del;383_384insGGTAGAAAATCAAAT;1539_1540ins129 |
39 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354149.1 | 83.9% | 83.9% | 1_153del;218_219ins93;1374_1375ins129 |
40 | human | 64693 | CTAGE1 | cutaneous T cell lymphoma-a... | NM_172241.3 | 83.9% | 79.3% | (many diffs) |
41 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354153.2 | 80.9% | 80.9% | 1_240del;305_306ins93;1461_1462ins129 |
42 | human | 643854 | CTAGE9 | CTAGE family member 9 | NM_001145659.1 | 79.4% | 72.1% | (many diffs) |
43 | human | 100142659 | CTAGE8 | CTAGE family member 8 | NM_001278507.1 | 79.3% | 72.2% | (many diffs) |
44 | human | 100128553 | CTAGE4 | CTAGE family member 4 | NM_198495.2 | 79.3% | 72% | (many diffs) |
45 | human | 441294 | CTAGE15 | CTAGE family member 15 | NM_001008747.2 | 79% | 72% | (many diffs) |
46 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001354155.2 | 79% | 78.3% | (many diffs) |
47 | human | 340307 | CTAGE6 | CTAGE family member 6 | NM_178561.5 | 78.9% | 71.7% | (many diffs) |
48 | human | 112268382 | LOC112268382 | cTAGE family member 4-like | XR_002959124.1 | 76.2% | (many diffs) | |
49 | human | 647288 | CTAGE11P | CTAGE family member 11, pse... | NR_027466.1 | 74.2% | (many diffs) | |
50 | human | 119437 | CTAGE7P | CTAGE family member 7, pseu... | NR_044994.1 | 71.2% | (many diffs) | |
51 | human | 220429 | CTAGE10P | CTAGE family member 10, pse... | NR_003268.3 | 67.8% | (many diffs) | |
52 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NR_148723.2 | 60.8% | (many diffs) | |
53 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NR_148722.2 | 59% | (many diffs) | |
54 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NR_148721.1 | 57.3% | (many diffs) | |
55 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449594.1 | 51.4% | 51.4% | 1_2064del |
56 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449593.1 | 51.2% | 51% | (many diffs) |
57 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449592.1 | 51.1% | 51.1% | 1_2064del;2223_2249del |
58 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | NM_001329214.3 | 51% | 51% | 1_2064del;2207_2208insGGTAGAAAATCAAAT |
59 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449595.1 | 48.1% | 48.1% | 1_2064del;2223_2249del;3405_3406ins129 |
60 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449596.1 | 48% | 48% | 1_2064del;2207_2208insGGTAGAAAATCAAAT;3363_3364ins129 |
61 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XR_002957555.1 | 35.2% | (many diffs) | |
62 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XM_024449597.1 | 35% | 34.2% | (many diffs) |
63 | human | 4253 | MIA2 | MIA SH3 domain ER export fa... | XR_002957554.1 | 19.6% | (many diffs) | |
64 | mouse | 338320 | Mia2 | melanoma inhibitory activity 2 | NM_146034.3 | 76.2% | 71.1% | (many diffs) |
65 | mouse | 338320 | Mia2 | melanoma inhibitory activity 2 | NM_001329047.1 | 43.6% | 40.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2256
- ORF length:
- 2187
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctttctgga ctaattgaag aaaaaagtaa actacttgaa aaatttagcc 121 ttgttcaaaa agagtatgaa ggctatgaag tagagtcatc tttaaaggat gccagctttg 181 agaaggaggc aacagaagca caaagtttgg aggtagaaaa tcaaatggca acctgtgaaa 241 agctgaacag gtccaattct gaacttgagg atgaaatact ctgtctagaa aaagagttaa 301 aagaagagaa atccaaacat tctgaacaag atgaattgat ggcggatatt tcaaaaagga 361 tacagtctct agaagatgag tcaaaatccc tcaaatcaca agtagctgaa gccaaaatga 421 ccttcaagat atttcaaatg aatgaagaac gactgaagat agcaataaaa gatgctttga 481 atgaaaattc tcaacttcag gaaagccaga aacagctttt gcaagaagct gaagtatgga 541 aagaacaagt gagtgaactt aataaacaga aagtaacatt tgaagactcc aaagtacatg 601 cagaacaagt tctaaatgat aaagaaagtc acatcaagac tctgactgaa cgcttgttaa 661 agatgaaaga ttgggctgct atgcttggag aagacataac ggatgatgat aacttggaat 721 tagaaatgaa cagtgaatcg gaaaatggtg cttacttaga taatcctcca aaaggagctt 781 tgaagaaact gattcatgct gctaagttaa atgcttcttt aaaaacctta gaaggagaaa 841 gaaaccaaat ttatattcag ttgtctgaag ttgataaaac aaaggaagag cttacagagc 901 atattaaaaa tcttcagact gaacaagcat ctttgcagtc agaaaacaca cattttgaaa 961 atgagaatca gaagcttcaa cagaaactta aagtaatgac tgaattatat caagaaaatg 1021 aaatgaaact ccacaggaaa ttaacagtag aggaaaatta tcggttagag aaagaagaga 1081 aactttctaa agtagatgaa aagatcagcc atgccactga agagctggag acctatagaa 1141 agcgagccaa agatcttgaa gaagaattgg agagaactat tcattcttat caagggcaga 1201 ttatttccca tgagaaaaaa gcacatgata attggttggc agctcggaat gctgaaagaa 1261 acctcaatga tttaaggaaa gaaaatgctc acaacagaca aaaattaact gaaacagagc 1321 ttaaatttga acttttagaa aaagatcctt atgcactcga tgttccaaat acagcatttg 1381 gcagagagca ttccccatat ggtccctcac cattgggttg gccttcatct gaaacaagag 1441 cttttctctc tcctccaact ttgttggagg gtccactcag actctcacct ttgcttccag 1501 ggggaggagg aagaggctca cgaggcccag ggaatcctct ggaccatcag attaccaatg 1561 aaagaggaga atcaagctgt gataggttaa ccgatcctca tagggctccc tctgacactg 1621 ggtctctgtc acctccatgg gaccaggacc gtaggatgat gtttcctccg ccaggacaat 1681 catatcctga ttcagccctt cctccacaaa ggcaagacag attttgttct aattctggta 1741 gactgtctgg accagcagaa ctcagaagtt ttaatatgcc ttctttggat aaaatggatg 1801 ggtcaatgcc ttcagaaatg gaatccagta gaaatgatac caaagatgat cttggtaatt 1861 taaatgtgcc tgattcatct ctccctgctg aaaatgaagc cactggccct ggctttgttc 1921 ctccacctct tgctccaatc agaggtccat tgtttccagt ggatgcaaga ggcccattct 1981 tgagaagagg acctcctttc cccccacctc ctccaggagc catgtttgga gcttctcgag 2041 attattttcc accaggggat ttcccaggtc caccacctgc tccatttgca atgagaaatg 2101 tctatccacc gaggggtttt cctccttacc ttcccccaag acctggattt ttccccccac 2161 ccccacattc tgaaggtaga agtgagttcc cctcaggttt gattccacct tcaaatgagc 2221 ctgctactga acatccagaa ccacagcaag aaaccttgcc aactttcttg tacaaagtgg 2281 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 2341 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta