Construct: ORF ccsbBroad304_11109

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005343.1_s304c1, BRDN0000403951
Derived from:
ccsbBroadEn_11109
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
RPS6KB1 (6198)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_11109
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001272043.2 100% 100%
2 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_003161.4 85.7% 85.1% (many diffs)
3 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 XM_011525102.3 84.5% 84.2% (many diffs)
4 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001272042.1 81.3% 80.7% (many diffs)
5 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001272060.1 81.3% 80.7% (many diffs)
6 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369669.1 78.8% 78.2% (many diffs)
7 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001272044.1 75.6% 75% (many diffs)
8 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369670.1 71.6% 71% (many diffs)
9 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369671.1 70.4% 69.9% (many diffs)
10 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369672.1 68.7% 68.1% (many diffs)
11 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369674.1 46.3% 42.3% (many diffs)
12 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369675.1 46.3% 42.3% (many diffs)
13 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369676.1 44.9% 42.5% (many diffs)
14 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369677.1 44.9% 42.5% (many diffs)
15 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369678.1 44.9% 42.5% (many diffs)
16 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369679.1 44.9% 42.5% (many diffs)
17 human 100287072 LOC100287072 ribosomal protein S6 kinase... NR_073509.1 34.8% (many diffs)
18 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NM_001369673.1 33.2% 31% (many diffs)
19 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161461.1 24.8% (many diffs)
20 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161456.1 24.8% (many diffs)
21 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161459.1 24.4% (many diffs)
22 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161458.1 24% (many diffs)
23 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161460.1 23.5% (many diffs)
24 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161457.1 23% (many diffs)
25 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161462.1 22.8% (many diffs)
26 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 NR_161455.1 22.1% (many diffs)
27 human 6198 RPS6KB1 ribosomal protein S6 kinase B1 XR_002958051.1 17.5% (many diffs)
28 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_011249275.2 94.4% 98.6% (many diffs)
29 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_011249276.2 89.6% 91.1% (many diffs)
30 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... NM_001114334.1 81.3% 84.7% (many diffs)
31 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_006534314.3 71.8% 75% (many diffs)
32 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_006534315.3 66.2% 69.5% (many diffs)
33 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_017314791.1 55.3% 39.8% (many diffs)
34 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_017314792.1 47% 40.2% (many diffs)
35 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_006534317.2 44.9% 46.4% (many diffs)
36 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_011249277.2 44.9% 46.4% (many diffs)
37 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XM_017314793.1 42.5% 42.7% (many diffs)
38 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XR_879743.2 28.9% (many diffs)
39 mouse 72508 Rps6kb1 ribosomal protein S6 kinase... XR_001780060.1 23.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1419
ORF length:
1353
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgag gcgacgaagg aggcgggacg gcttttaccc agccccggac ttccgagaca
121gggaagctga ggacatggca ggagtgtttg acatagacct ggaccagcca gaggacgcgg
181gctctgagga tgagctggag gaggggggtc agttaaatga aagcatggac catgggggag
241ttggaccata tgaacttggc atggaacatt gtgagaaatt tgaaatctca gaaactagtg
301tgaacagagg gccagaaaaa atcagaccag aatgttttga gctacttcgg gtacttggta
361aagggggcta tggaaaggtt tttcaagtac gaaaagtaac aggagcaaat actgggaaaa
421tatttgccat gaaggtgctt aaaaaggcaa tgatagtaag aaatgctaaa gatacagctc
481atacaaaagc agaacggaat attctggagg aagtaaagca tcccttcatc gtggatttaa
541tttatgcctt tcagactggt ggaaaactct acctcatcct tgagtatctc agtggaggag
601aactatttat gcagttagaa agagagggaa tatttatgga agacactgcc tgcttttact
661tggcagaaat ctccatggct ttggggcatt tacatcaaaa ggggatcatc tacagagacc
721tgaagccgga gaatatcatg cttaatcacc aaggtcatgt gaaactaaca gactttggac
781tatgcaaaga atctattcat gatggaacag tcacacacac attttgtgga acaatagaat
841acatggcccc tgaaatcttg atgagaagtg gccacaatcg tgctgtggat tggtggagtt
901tgggagcatt aatgtatgac atgctgactg gagcaccccc attcactggg gagaatagaa
961agaaaacaat tgacaaaatc ctcaaatgta aactcaattt gcctccctac ctcacacaag
1021aagccagaga tctgcttaaa aagctgctga aaagaaatgc tgcttctcgt ctgggagctg
1081gtcctgggga cgctggagaa gttcaagctc atccattctt tagacacatt aactgggaag
1141aacttctggc tcgaaaggtg gagcccccct ttaaacctct gttgcaatct gaagaggatg
1201taagtcagtt tgattccaag tttacacgtc agacacctgt cgacagccca gatgactcaa
1261ctctcagtga aagtgccaat caggtctttc tgggttttac atatgtggct ccatctgtac
1321ttgaaagtgt gaaagaaaag ttttcctttg aaccaaaaat ccgatcacct cgaagattta
1381ttggcagccc acgaacacct gtcagtactg ctatgtgcta cccaactttc ttgtacaaag
1441tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta
1501gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.