Construct: ORF ccsbBroad304_12038

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015529.1_s304c1, BRDN0000399444
Derived from:
ccsbBroadEn_12038
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
STAG3L1 (54441)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12038
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 442582 STAG3L2 stromal antigen 3-like 2 (p... NR_040584.2 12.5% 1_738del;771C>T;976_1874del
2 human 378108 TRIM74 tripartite motif containing 74 XR_002956445.1 11.6% (many diffs)
3 human 54441 STAG3L1 stromal antigen 3-like 1 (p... NR_040583.2 11% 1_994del;1232_2138del
4 human 442578 STAG3L3 stromal antigen 3-like 3 (p... NR_040582.2 10.9% 1_1013del;1251_2167del
5 human 378108 TRIM74 tripartite motif containing 74 XR_001744735.1 10.9% 1_646del;884_2172del
6 human 378108 TRIM74 tripartite motif containing 74 XR_001744750.1 10.5% 1_725del;963_2251del
7 human 378108 TRIM74 tripartite motif containing 74 XR_001744736.1 10.4% 1_734del;972_2260del
8 human 378108 TRIM74 tripartite motif containing 74 XR_001744739.2 9.8% 1_878del;1116_2404del
9 human 378108 TRIM74 tripartite motif containing 74 XR_001744737.2 9.6% 1_918del;1156_2444del
10 human 10734 STAG3 stromal antigen 3 NM_001282718.2 6.2% 5.1% (many diffs)
11 human 10734 STAG3 stromal antigen 3 XM_017011687.1 6.1% 5.1% (many diffs)
12 human 10734 STAG3 stromal antigen 3 NM_001282716.1 5.9% 4.9% (many diffs)
13 human 10734 STAG3 stromal antigen 3 NM_012447.4 5.9% 4.9% (many diffs)
14 human 10734 STAG3 stromal antigen 3 NM_001282717.1 5.9% 4.9% (many diffs)
15 human 10734 STAG3 stromal antigen 3 XM_011515742.1 5.9% 4.9% (many diffs)
16 human 10734 STAG3 stromal antigen 3 XM_017011686.2 5.8% 4.8% (many diffs)
17 human 10734 STAG3 stromal antigen 3 XM_017011683.2 5.8% 4.8% (many diffs)
18 human 10734 STAG3 stromal antigen 3 XM_017011684.1 5.8% 4.8% (many diffs)
19 human 10734 STAG3 stromal antigen 3 XM_017011685.1 5.8% 4.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
303
ORF length:
237
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg tggaagagag caacgccaga gcccaggtgc ccagaggact ttcttccagc
121ttctgctgtc cttctttgtg gagagcaagc tccacgacca cgctgcttac ttagtagaca
181acctgtggga ctgtgcaggg actcagctga aggactggga gggtctgaca agcctgctgc
241tggagaagga ccagagcacg tgccacatgg agccagggcc agggaccttc cacctcctag
301ggtgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
361tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
421tctggatta

Download FASTA (ORF) (Full)