Transcript: Human XR_001744739.2

PREDICTED: Homo sapiens tripartite motif containing 74 (TRIM74), transcript variant X17, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
TRIM74 (378108)
Length:
2404
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001744739.2
NBCI Gene record:
TRIM74 (378108)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001744739.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000121694 CCTCACCGACAGCTATTTAAA pLKO.1 512 3UTR 100% 15.000 7.500 Y STAG3L3 n/a
2 TRCN0000359633 CGCCCAGCATGTTAGACAATT pLKO_005 1485 3UTR 100% 13.200 6.600 Y STAG3L1 n/a
3 TRCN0000062688 GCCCAGCATGTTAGACAATTT pLKO.1 1486 3UTR 100% 13.200 6.600 Y STAG3L1 n/a
4 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 2300 3UTR 100% 13.200 6.600 Y LIAS n/a
5 TRCN0000062692 GCTATCTGCATTGAGGAAATT pLKO.1 459 3UTR 100% 13.200 6.600 Y STAG3L1 n/a
6 TRCN0000139360 CAGCTTCTGCTGTCCTTCTTT pLKO.1 930 3UTR 100% 5.625 2.813 Y STAG3L3 n/a
7 TRCN0000167398 GAATTTCTGTACTGGAAACTT pLKO.1 831 3UTR 100% 5.625 2.813 Y STAG3L2 n/a
8 TRCN0000062689 GCCGTCAGATTACTGATACTT pLKO.1 711 3UTR 100% 5.625 2.813 Y STAG3L1 n/a
9 TRCN0000135957 GCCGTCAGATTACTGATACTT pLKO.1 711 3UTR 100% 5.625 2.813 Y STAG3 n/a
10 TRCN0000142277 GCCGTCAGATTACTGATACTT pLKO.1 711 3UTR 100% 5.625 2.813 Y STAG3L3 n/a
11 TRCN0000062690 CGCTGCTTACTTAGTAGACAA pLKO.1 974 3UTR 100% 4.950 2.475 Y STAG3L1 n/a
12 TRCN0000167941 CGCTGCTTACTTAGTAGACAA pLKO.1 974 3UTR 100% 4.950 2.475 Y STAG3L2 n/a
13 TRCN0000168487 GAGAGATTGCTTGCTTCACTT pLKO.1 1125 3UTR 100% 4.950 2.475 Y STAG3L2 n/a
14 TRCN0000139995 GAGATCCGTGCTATCTGCATT pLKO.1 450 3UTR 100% 4.950 2.475 Y STAG3L3 n/a
15 TRCN0000062691 GCAAAGCTACAGCACGTCTTT pLKO.1 491 3UTR 100% 4.950 2.475 Y STAG3L1 n/a
16 TRCN0000122408 GCAAAGCTACAGCACGTCTTT pLKO.1 491 3UTR 100% 4.950 2.475 Y STAG3L3 n/a
17 TRCN0000138869 GCAAAGCTACAGCACGTCTTT pLKO.1 491 3UTR 100% 4.950 2.475 Y STAG3 n/a
18 TRCN0000172776 GCTTCAAGGACTGGATGGTTT pLKO.1 652 3UTR 100% 4.950 2.475 Y STAG3L2 n/a
19 TRCN0000139256 CTGCATGATAAGCACCGAGAA pLKO.1 549 3UTR 100% 4.050 2.025 Y STAG3L3 n/a
20 TRCN0000140844 CAGAGGACTTTCTTCCAGCTT pLKO.1 915 3UTR 100% 2.640 1.320 Y STAG3L3 n/a
21 TRCN0000142181 GTTTCCATGATCATGGACAGA pLKO.1 669 3UTR 100% 0.264 0.132 Y STAG3L3 n/a
22 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1451 3UTR 100% 5.625 2.813 Y KLHL30 n/a
23 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1780 3UTR 100% 4.950 2.475 Y KAAG1 n/a
24 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1451 3UTR 100% 5.625 2.813 Y EID2B n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001744739.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10171 pDONR223 100% 16.7% None 1_392del;795_2404del n/a
2 ccsbBroad304_10171 pLX_304 0% 16.7% V5 1_392del;795_2404del n/a
3 TRCN0000474301 GGCATGATAACGCGACACGTACAC pLX_317 60.6% 16.7% V5 1_392del;795_2404del n/a
4 ccsbBroadEn_13781 pDONR223 100% 10.1% None (many diffs) n/a
5 ccsbBroad304_13781 pLX_304 0% 10.1% V5 (many diffs) n/a
6 TRCN0000469746 TCCCTGCGCCGTCCGGGTTTTCGA pLX_317 100% 10.1% V5 (many diffs) n/a
7 ccsbBroadEn_13704 pDONR223 100% 9.9% None (many diffs) n/a
8 ccsbBroad304_13704 pLX_304 0% 9.9% V5 (many diffs) n/a
9 ccsbBroadEn_12038 pDONR223 100% 9.8% None 1_878del;1116_2404del n/a
10 ccsbBroad304_12038 pLX_304 0% 9.8% V5 1_878del;1116_2404del n/a
11 TRCN0000471778 CAAGTTTGACTAGCTTGAAGTGGC pLX_317 100% 9.8% V5 1_878del;1116_2404del n/a
Download CSV