Construct: ORF ccsbBroad304_13398

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007708.1_s304c1, BRDN0000408048
Derived from:
ccsbBroadEn_13398
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TUBB (203068)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13398
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 203068 TUBB tubulin beta class I NM_001293213.2 51.1% 50% 1_344delinsA;350_352delTGG;355_362delGTGGTACG
2 human 347688 TUBB8 tubulin beta 8 class VIII XM_011519460.2 35.4% 36.9% (many diffs)
3 human 84617 TUBB6 tubulin beta 6 class V NM_001303529.2 34.6% 36% (many diffs)
4 human 84617 TUBB6 tubulin beta 6 class V NM_001303530.2 34.6% 36% (many diffs)
5 human 203068 TUBB tubulin beta class I NM_001293215.2 33.3% 33.3% 1_744del
6 human 203068 TUBB tubulin beta class I NM_001293216.2 33.3% 33.3% 1_744del
7 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016192.2 31.1% 32.4% (many diffs)
8 human 347688 TUBB8 tubulin beta 8 class VIII XM_017016193.2 31.1% 32.4% (many diffs)
9 human 203068 TUBB tubulin beta class I NM_001293214.2 31% 31% 1_828del
10 human 7280 TUBB2A tubulin beta 2A class IIa NM_001310315.2 30.1% 31.9% (many diffs)
11 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289130.1 28.9% 31.3% (many diffs)
12 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289131.1 28.9% 31.3% (many diffs)
13 human 10381 TUBB3 tubulin beta 3 class III NM_001197181.2 27.9% 29.3% (many diffs)
14 human 203068 TUBB tubulin beta class I NM_178014.4 27.9% 27.9% 1_960del
15 human 84617 TUBB6 tubulin beta 6 class V NM_001303527.2 27.8% 28.8% (many diffs)
16 human 347688 TUBB8 tubulin beta 8 class VIII XM_011519459.3 27.8% 28.9% (many diffs)
17 human 84617 TUBB6 tubulin beta 6 class V NM_001303528.2 27.3% 28.3% (many diffs)
18 human 203068 TUBB tubulin beta class I NM_001293212.2 26.7% 26.7% 1_1020del
19 human 84617 TUBB6 tubulin beta 6 class V NM_001303526.2 25.4% 26.4% (many diffs)
20 human 10383 TUBB4B tubulin beta 4B class IVb NM_006088.6 24.4% 26.5% (many diffs)
21 human 7280 TUBB2A tubulin beta 2A class IIa NM_001069.3 24.4% 25.8% (many diffs)
22 human 347733 TUBB2B tubulin beta 2B class IIb NM_178012.5 24.4% 25.8% (many diffs)
23 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289129.1 24.2% 26.2% (many diffs)
24 human 10382 TUBB4A tubulin beta 4A class IVa NM_006087.4 24.2% 26.2% (many diffs)
25 human 10381 TUBB3 tubulin beta 3 class III NM_006086.4 23.5% 24.6% (many diffs)
26 human 84617 TUBB6 tubulin beta 6 class V NM_001303524.1 23.3% 24.2% (many diffs)
27 human 84617 TUBB6 tubulin beta 6 class V NM_032525.3 23.3% 24.2% (many diffs)
28 human 347688 TUBB8 tubulin beta 8 class VIII NM_177987.2 23.3% 24.2% (many diffs)
29 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289127.1 22% 23.8% (many diffs)
30 human 10382 TUBB4A tubulin beta 4A class IVa NM_001289123.1 21.7% 23.5% (many diffs)
31 human 203068 TUBB tubulin beta class I NR_120608.2 19.4% 1_516del;889_1916del
32 mouse 22154 Tubb5 tubulin, beta 5 class I NM_011655.5 26.2% 27.9% (many diffs)
33 mouse 22151 Tubb2a tubulin, beta 2A class IIA NM_009450.2 25.1% 25.8% (many diffs)
34 mouse 73710 Tubb2b tubulin, beta 2B class IIB NM_023716.2 25% 25.8% (many diffs)
35 mouse 22153 Tubb4a tubulin, beta 4A class IVA NM_009451.3 24.4% 26.2% (many diffs)
36 mouse 67951 Tubb6 tubulin, beta 6 class V NM_026473.2 23.9% 24.6% (many diffs)
37 mouse 22152 Tubb3 tubulin, beta 3 class III NM_023279.2 23.8% 24.4% (many diffs)
38 mouse 227613 Tubb4b tubulin, beta 4B class IVB NM_146116.2 23.8% 26.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
438
ORF length:
372
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc catgaaggag gtcgatgagc agatgcttaa cgtgcagaac aagaacagca
121gctactttgt ggaatggatc cccaacaatg tcaagacagc cgtctgtgac atcccacctc
181gtggcctcaa gatggcagtc accttcattg gcaatagcac agccatccag gagctcttca
241agcgcatctc ggagcagttc actgccatgt tccgccggaa ggccttcctc cactggtaca
301caggcgaggg catggacgag atggagttca ccgaggctga gagcaacatg aacgacctcg
361tctctgagta tcagcagtac caggatgcca ccgcagaaga ggaggaggat ttcggtgagg
421aggccgaaga ggaggcctac ccaactttct tgtacaaagt ggttggtaag cctatcccta
481accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta
541agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)