Construct: ORF ccsbBroad304_15336
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018802.1_s304c1, BRDN0000459286
- Derived from:
- ccsbBroadEn_15336
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC650122 (650122)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_15336
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1119 | CHKA | choline kinase alpha | XM_017017148.2 | 86.9% | 84.6% | (many diffs) |
| 2 | human | 1119 | CHKA | choline kinase alpha | NM_212469.1 | 71% | 69.4% | (many diffs) |
| 3 | human | 1119 | CHKA | choline kinase alpha | NM_001277.3 | 68.3% | 66.7% | (many diffs) |
| 4 | human | 1119 | CHKA | choline kinase alpha | XM_017017147.1 | 64.9% | 60.9% | (many diffs) |
| 5 | human | 1119 | CHKA | choline kinase alpha | XR_428904.3 | 47.2% | (many diffs) | |
| 6 | human | 1119 | CHKA | choline kinase alpha | XR_949772.2 | 32.6% | (many diffs) | |
| 7 | human | 1119 | CHKA | choline kinase alpha | XR_949773.2 | 29.7% | (many diffs) | |
| 8 | human | 1119 | CHKA | choline kinase alpha | XR_428905.3 | 10.8% | (many diffs) | |
| 9 | mouse | 12660 | Chka | choline kinase alpha | XM_006531648.2 | 75.2% | 74.6% | (many diffs) |
| 10 | mouse | 12660 | Chka | choline kinase alpha | XM_006531649.2 | 75.2% | 74.6% | (many diffs) |
| 11 | mouse | 12660 | Chka | choline kinase alpha | XM_006531650.2 | 75.2% | 74.6% | (many diffs) |
| 12 | mouse | 12660 | Chka | choline kinase alpha | XM_006531651.3 | 75.2% | 74.6% | (many diffs) |
| 13 | mouse | 12660 | Chka | choline kinase alpha | XM_011248599.2 | 75.2% | 74.6% | (many diffs) |
| 14 | mouse | 12660 | Chka | choline kinase alpha | XM_011248600.2 | 75.2% | 74.6% | (many diffs) |
| 15 | mouse | 12660 | Chka | choline kinase alpha | XM_017318048.1 | 75.2% | 74.6% | (many diffs) |
| 16 | mouse | 12660 | Chka | choline kinase alpha | XM_017318049.1 | 75.2% | 74.6% | (many diffs) |
| 17 | mouse | 12660 | Chka | choline kinase alpha | XM_017318050.1 | 75.2% | 74.6% | (many diffs) |
| 18 | mouse | 12660 | Chka | choline kinase alpha | NM_013490.4 | 62% | 61.6% | (many diffs) |
| 19 | mouse | 12660 | Chka | choline kinase alpha | NM_001271496.1 | 59.6% | 59.2% | (many diffs) |
| 20 | mouse | 12660 | Chka | choline kinase alpha | XM_006531646.1 | 58.3% | 57.9% | (many diffs) |
| 21 | mouse | 12660 | Chka | choline kinase alpha | XR_388248.2 | 35.4% | (many diffs) | |
| 22 | mouse | 12660 | Chka | choline kinase alpha | NR_073190.1 | 30.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1029
- ORF length:
- 960
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gctgttccag tgctccctac ctgacaccac agccaccctt ggtgatgagc 121 ctcggaaagt gctcctgcgg ctgtatggag cgattttgca gatgggggct gaggccatgg 181 ttctggagag cgttatgttt gccattctcg cagagaggtc acttgggcca aaactctatg 241 gcatctttcc ccaaggccga ctggagcagt tcatcccgag ccggcgatta gatactgaag 301 aattaagttt gccagatatt tctgcagaaa tcgccgagaa aatggctaca tttcatggta 361 tgaaaatgcc attcaataag gaaccaaaat ggctttttgg cacaatggaa aagtatctaa 421 aggaagtgct gagaattaaa tttactgagg aatccagaat taaaaagctc cacaaattgc 481 tcagttacaa tctgcccttg gaactggaaa acctgagatc attgcttgaa tctactccat 541 ctccagttgt attttgtcat aatgactgtc aagaaggtaa tatcttgttg ctggaaggcc 601 gagagaattc tgaaaaacag aaactgatgc tcattgattt cgaatacagc agttacaatt 661 acaggggatt cgacattgga aatcacttct gtgagtggat gtatgattat agctatgaaa 721 aatacccttt tttcagagca annnnccgga agtatcccac caagaaacaa cagctccatt 781 ttatttccag ttacttgcct gcattccaaa atgactttga aaacctcagt actgaagaaa 841 aatccattat aaaagaagaa atgttgcttg aagttaatag gtttgccctt gcatctcatt 901 tcctctgggg actgtggtcc attgtacaag ccaagatttc atctattgaa tttgggtaca 961 tgnncnacgc ccaagcaagg tttgatgcct atttccagtt acttgccngn agaggaagct 1021 tggggtgttn cnaantnnct tgtacaaagt ggttggtaag cctatcccta accctctcct 1081 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat 1141 caacctctgg atta