Transcript: Human NM_001321685.2

Homo sapiens zinc finger protein 225 (ZNF225), transcript variant 2, mRNA.

Source:
NCBI, updated 2019-05-31
Taxon:
Homo sapiens (human)
Gene:
ZNF225 (7768)
Length:
4612
CDS:
405..2525

Additional Resources:

NCBI RefSeq record:
NM_001321685.2
NBCI Gene record:
ZNF225 (7768)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001321685.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000013076 CCGTAGTAGAGCAAATCTTAA pLKO.1 1295 CDS 100% 13.200 9.240 N ZNF225 n/a
2 TRCN0000013077 CATCAATCACTCCACAGAGAT pLKO.1 549 CDS 100% 4.950 3.465 N ZNF225 n/a
3 TRCN0000013073 CGCTTCATTTATAGGCAAGAT pLKO.1 1458 CDS 100% 4.950 3.465 N ZNF225 n/a
4 TRCN0000013074 GAAGGCTTGTTCAGTCATCAA pLKO.1 690 CDS 100% 4.950 3.465 N ZNF225 n/a
5 TRCN0000013075 CACAACTTTATACCCATCGTA pLKO.1 1894 CDS 100% 3.000 2.100 N ZNF225 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001321685.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_01821 pDONR223 100% 100% 100% None n/a
2 ccsbBroad304_01821 pLX_304 0% 100% 100% V5 n/a
3 TRCN0000468268 AGACCACTGATCAAGATCTATTTA pLX_317 18.1% 100% 100% V5 n/a
4 ccsbBroadEn_07177 pDONR223 100% 79.1% 71.2% None (many diffs) n/a
5 ccsbBroad304_07177 pLX_304 0% 79.1% 71.2% V5 (many diffs) n/a
6 TRCN0000469087 TAGTTGACACCGCGGTGTCCATGT pLX_317 21.4% 79.1% 71.2% V5 (many diffs) n/a
7 ccsbBroadEn_07162 pDONR223 100% 70.6% 59% None (many diffs) n/a
8 ccsbBroad304_07162 pLX_304 0% 70.6% 59% V5 (many diffs) n/a
9 TRCN0000474103 CGTATCCGGTAAACAACTGTACCC pLX_317 13.6% 70.6% 59% V5 (many diffs) n/a
10 ccsbBroadEn_07167 pDONR223 100% 66.2% 58.4% None (many diffs) n/a
11 ccsbBroad304_07167 pLX_304 0% 66.2% 58.4% V5 (many diffs) n/a
12 TRCN0000472100 CATTCGAGTACGACGCCCGCGCAT pLX_317 28.8% 66.2% 58.4% V5 (many diffs) n/a
13 ccsbBroadEn_01823 pDONR223 100% 57.5% 49.8% None (many diffs) n/a
14 ccsbBroad304_01823 pLX_304 0% 57.5% 49.8% V5 (many diffs) n/a
15 TRCN0000476788 CTGCGAAGACGGCAACATCAGATA pLX_317 29.8% 57.5% 49.8% V5 (many diffs) n/a
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