Transcript: Mouse NM_009608.4

Mus musculus actin, alpha, cardiac muscle 1 (Actc1), mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Actc1 (11464)
Length:
1461
CDS:
144..1277

Additional Resources:

NCBI RefSeq record:
NM_009608.4
NBCI Gene record:
Actc1 (11464)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_009608.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000090092 CGATGGTGTAACTCATAATGT pLKO.1 617 CDS 100% 5.625 4.500 N Actc1 n/a
2 TRCN0000090090 GATGGTGTAACTCATAATGTT pLKO.1 618 CDS 100% 5.625 4.500 N Actc1 n/a
3 TRCN0000090091 CGTAAATACTCTGTCTGGATT pLKO.1 1152 CDS 100% 4.950 3.465 N Actc1 n/a
4 TRCN0000090088 TCTCTCTCTTAGCCTACCTTA pLKO.1 1282 3UTR 100% 4.950 3.465 N Actc1 n/a
5 TRCN0000090089 CGTGAAATTGTCCGTGACATA pLKO.1 765 CDS 100% 4.950 2.970 N Actc1 n/a
6 TRCN0000090902 CCAGATCATGTTTGAGACCTT pLKO.1 509 CDS 100% 2.640 1.320 Y Actb n/a
7 TRCN0000083554 CGTGAAATTGTCCGTGACATT pLKO.1 765 CDS 100% 4.950 2.970 N ACTC1 n/a
8 TRCN0000090841 GCAAAGACCTGTATGCCAATA pLKO.1 1018 CDS 100% 10.800 5.400 Y Actg1 n/a
9 TRCN0000335353 GCAAAGACCTGTATGCCAATA pLKO_005 1018 CDS 100% 10.800 5.400 Y Actg1 n/a
10 TRCN0000089839 CGCAAAGACCTGTATGCCAAT pLKO.1 1017 CDS 100% 4.050 2.025 Y Actg-ps1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_009608.4, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00015 pDONR223 100% 92.3% 100% None (many diffs) n/a
2 ccsbBroad304_00015 pLX_304 0% 92.3% 100% V5 (many diffs) n/a
3 TRCN0000468012 TCTATATTCTTCCCTCACTCATAA pLX_317 37.9% 92.3% 100% V5 (many diffs) n/a
4 ccsbBroadEn_00014 pDONR223 100% 84.3% 98.4% None (many diffs) n/a
5 ccsbBroad304_00014 pLX_304 0% 84.3% 98.4% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000467679 GCTACTGCTTTGAGGGTTATAACT pLX_317 37.9% 84.3% 98.4% V5 (not translated due to prior stop codon) (many diffs) n/a
7 ccsbBroadEn_05763 pDONR223 100% 82.7% 93.1% None (many diffs) n/a
8 ccsbBroad304_05763 pLX_304 53.1% 82.7% 93.1% V5 (many diffs) n/a
9 TRCN0000470279 TCAAGCCTGGAACGGGCTCACGTC pLX_317 31.7% 82.7% 93.1% V5 (many diffs) n/a
10 ccsbBroadEn_15351 pDONR223 0% 81.9% 93.6% None (many diffs) n/a
11 ccsbBroad304_15351 pLX_304 0% 81.9% 93.6% V5 (many diffs) n/a
12 ccsbBroadEn_13808 pDONR223 100% 81.9% 93.1% None (many diffs) n/a
13 ccsbBroad304_13808 pLX_304 0% 81.9% 93.1% V5 (not translated due to frame shift) (many diffs) n/a
14 TRCN0000471742 AATGGGAGTTCTCACGTCAGGTCC pLX_317 44.8% 81.9% 93.1% V5 (not translated due to frame shift) (many diffs) n/a
15 ccsbBroadEn_05764 pDONR223 100% 81.8% 93.6% None (many diffs) n/a
16 ccsbBroad304_05764 pLX_304 0% 81.8% 93.6% V5 (many diffs) n/a
17 TRCN0000466781 AATTGTGGTGCTTGTTGCCGCCTG pLX_317 31.7% 81.8% 93.6% V5 (many diffs) n/a
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