Construct: ORF TRCN0000468012

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006671.1_s317c1
Derived from:
ccsbBroadEn_00015
DNA Barcode:
TCTATATTCTTCCCTCACTCATAA
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACTC1 (70)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000468012
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 70 ACTC1 actin alpha cardiac muscle 1 NM_005159.5 100% 100%
2 human 72 ACTG2 actin gamma 2, smooth muscle NM_001615.4 85.9% 98.6% (many diffs)
3 human 58 ACTA1 actin alpha 1, skeletal muscle NM_001100.4 85.6% 98.9% (many diffs)
4 human 59 ACTA2 actin alpha 2, smooth muscle NM_001141945.2 85.6% 98.4% (many diffs)
5 human 59 ACTA2 actin alpha 2, smooth muscle NM_001320855.1 85.6% 98.4% (many diffs)
6 human 59 ACTA2 actin alpha 2, smooth muscle NM_001613.4 85.6% 98.4% (many diffs)
7 human 60 ACTB actin beta NM_001101.5 84.8% 93.3% (many diffs)
8 human 71 ACTG1 actin gamma 1 NM_001199954.2 83% 93.6% (many diffs)
9 human 71 ACTG1 actin gamma 1 NM_001614.5 83% 93.6% (many diffs)
10 human 72 ACTG2 actin gamma 2, smooth muscle NM_001199893.2 76.6% 87.2% (many diffs)
11 human 653269 POTEI POTE ankyrin domain family ... XM_017004734.2 47.4% 49.4% (many diffs)
12 human 728378 POTEF POTE ankyrin domain family ... XM_017004835.1 47.1% 49.4% (many diffs)
13 human 653781 POTEJ POTE ankyrin domain family ... XM_017004743.2 47.1% 49.1% (many diffs)
14 human 653269 POTEI POTE ankyrin domain family ... XM_017004733.1 46.1% 48.1% (many diffs)
15 human 728378 POTEF POTE ankyrin domain family ... XM_017004834.1 45.8% 48.1% (many diffs)
16 human 653781 POTEJ POTE ankyrin domain family ... XM_017004742.1 45.8% 47.8% (many diffs)
17 human 445582 POTEE POTE ankyrin domain family ... XM_011511216.2 32.7% 34.2% (many diffs)
18 human 445582 POTEE POTE ankyrin domain family ... XM_017004159.2 32.4% 33.9% (many diffs)
19 human 653781 POTEJ POTE ankyrin domain family ... NM_001277083.2 29.9% 31.1% (many diffs)
20 human 653781 POTEJ POTE ankyrin domain family ... XM_011511683.2 29.6% 30.8% (many diffs)
21 human 653781 POTEJ POTE ankyrin domain family ... XM_017004741.2 29.6% 30.8% (many diffs)
22 human 445582 POTEE POTE ankyrin domain family ... NM_001083538.2 29.1% 30.5% (many diffs)
23 human 445582 POTEE POTE ankyrin domain family ... XM_017004161.2 29.1% 30.5% (many diffs)
24 human 653269 POTEI POTE ankyrin domain family ... NM_001277406.2 29% 30.2% (many diffs)
25 human 653269 POTEI POTE ankyrin domain family ... XM_024453046.1 29% 30.2% (many diffs)
26 human 728378 POTEF POTE ankyrin domain family ... NM_001099771.2 28.8% 30.2% (many diffs)
27 human 653269 POTEI POTE ankyrin domain family ... XM_011511670.2 28.7% 29.9% (many diffs)
28 human 653269 POTEI POTE ankyrin domain family ... XM_017004732.2 28.7% 29.9% (many diffs)
29 human 728378 POTEF POTE ankyrin domain family ... XM_017004833.1 28.6% 29.9% (many diffs)
30 human 653269 POTEI POTE ankyrin domain family ... NM_001371926.1 28.4% 29.6% (many diffs)
31 mouse 11464 Actc1 actin, alpha, cardiac muscle 1 NM_009608.4 92.3% 100% (many diffs)
32 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_001272041.1 87.7% 98.9% (many diffs)
33 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_009606.3 87.7% 98.9% (many diffs)
34 mouse 11468 Actg2 actin, gamma 2, smooth musc... NM_009610.2 85.6% 98.6% (many diffs)
35 mouse 11461 Actb actin, beta NM_007393.5 84.8% 93.3% (many diffs)
36 mouse 11475 Acta2 actin, alpha 2, smooth musc... NM_007392.3 84% 98.4% (many diffs)
37 mouse 11465 Actg1 actin, gamma, cytoplasmic 1 NM_009609.3 83.1% 93.6% (many diffs)
38 mouse 11475 Acta2 actin, alpha 2, smooth musc... XM_006526606.2 62.9% 71% (many diffs)
39 mouse 11465 Actg1 actin, gamma, cytoplasmic 1 NM_001313923.1 56.5% 63.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1197
ORF length:
1131
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgtg tgacgacgag gagaccaccg ccctggtgtg cgacaacggc tctgggctgg
121tgaaggccgg ctttgcgggc gatgacgcgc cccgcgctgt cttcccgtcc atcgtgggcc
181gcccgcggca ccagggagtt atggtgggta tgggtcagaa ggactcctac gtaggtgatg
241aagcccagag caagagaggc atcctgaccc tgaagtatcc catcgagcat ggtatcatca
301ccaactggga cgacatggag aagatctggc accacacctt ctacaatgag ctccgtgtgg
361ctcccgagga gcaccccacc ctgctcacag aggccccgct gaaccccaag gccaaccggg
421agaagatgac tcagatcatg tttgagacct tcaatgtccc tgccatgtac gtggccatcc
481aggcagtgct atccctgtat gcttctggcc gtaccacagg cattgttctg gactctgggg
541atggtgtaac tcacaatgtc cccatctatg agggctacgc tttgccccat gccatcatgc
601gtctggatct ggctggtcgg gacctcactg actacctcat gaagatcctc actgagcgtg
661gctactcctt tgtcaccact gctgaacgtg aaattgtccg tgacattaaa gagaagctgt
721gctatgtcgc cctggatttt gagaatgaga tggccacagc tgcctcttcc TCCTCCCTGG
781AGAAGAGCTA TGAACTGCCT GATGGCCAAG TCATCACTAT TGGCAATGAG CGCTTCCGCT
841GTCCTGAGAC ACTCTTCCAG CCCTCCTTCA TTGGTATGGA ATCTGCTGGC ATCCATGAAA
901CAACTTACAA TAGCATCATG AAGTGTGACA TTGATATCCG CAAGGACCTG TATGCCAACA
961ATGTCTTATC TGGAGGCACC ACTATGTACC CTGGTATTGC TGATCGTATG CAGAAGGAAA
1021TCACTGCTCT GGCTCCTAGC ACCATGAAGA TTAAGATTAT TGCTCCCCCT GAGCGTAAAT
1081ACTCTGTCTG GATTGGGGGC TCCATCCTGG CCTCTCTGTC CACCTTCCAG CAAATGTGGA
1141TTAGCAAGCA AGAGTACGAT GAGGCAGGCC CATCCATTGT CCACCGCAAA TGCTTCTACC
1201CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC
1261TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT
1321ATATATCTTG TGGAAAGGAC GATCTATATT CTTCCCTCAC TCATAAACGC GTTAAGTCga
1381caatcaacct ctggattaca aaatttgtga aagatt

Download FASTA (ORF) (Full)