Construct: ORF ccsbBroadEn_15351

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000853.1_s300c1, BRDN0000388354
DNA Barcode:
None
Epitope Tag:
None
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACTG1 (71)

Vector Information:

Vector Backbone:
pDONR223
Pol II Cassette 1:
n/a
Pol II Cassette 2:
n/a
Selection Marker:
n/a
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 71 ACTG1 actin gamma 1 NM_001199954.2 99.9% 100% 918C>T
2 human 71 ACTG1 actin gamma 1 NM_001614.5 99.9% 100% 918C>T
3 human 60 ACTB actin beta NM_001101.5 91.2% 98.9% (many diffs)
4 human 58 ACTA1 actin alpha 1, skeletal muscle NM_001100.4 85.7% 93.1% (many diffs)
5 human 70 ACTC1 actin alpha cardiac muscle 1 NM_005159.5 82.9% 93.6% (many diffs)
6 human 72 ACTG2 actin gamma 2, smooth muscle NM_001615.4 82.3% 93.8% (many diffs)
7 human 59 ACTA2 actin alpha 2, smooth muscle NM_001141945.2 82.2% 93.6% (many diffs)
8 human 59 ACTA2 actin alpha 2, smooth muscle NM_001320855.1 82.2% 93.6% (many diffs)
9 human 59 ACTA2 actin alpha 2, smooth muscle NM_001613.4 82.2% 93.6% (many diffs)
10 human 345651 ACTBL2 actin beta like 2 NM_001017992.4 80.8% 90.9% (many diffs)
11 human 72 ACTG2 actin gamma 2, smooth muscle NM_001199893.2 72.8% 82.7% (many diffs)
12 human 71 ACTG1 actin gamma 1 NR_037688.3 63.5% 1_72del;990C>T;1198_1770del
13 human 653269 POTEI POTE ankyrin domain family ... XM_017004734.2 50.3% 51.7% (many diffs)
14 human 653781 POTEJ POTE ankyrin domain family ... XM_017004743.2 50.2% 51.4% (many diffs)
15 human 728378 POTEF POTE ankyrin domain family ... XM_017004835.1 50.1% 51.9% (many diffs)
16 human 648740 ACTG1P4 actin gamma 1 pseudogene 4 NR_024438.2 49.1% (many diffs)
17 human 653269 POTEI POTE ankyrin domain family ... XM_017004733.1 49% 50.3% (many diffs)
18 human 653781 POTEJ POTE ankyrin domain family ... XM_017004742.1 48.8% 50% (many diffs)
19 human 728378 POTEF POTE ankyrin domain family ... XM_017004834.1 48.8% 50.5% (many diffs)
20 human 644936 LOC644936 actin beta pseudogene NR_004845.2 39.9% (many diffs)
21 human 445582 POTEE POTE ankyrin domain family ... XM_011511216.2 34.6% 35.8% (many diffs)
22 human 445582 POTEE POTE ankyrin domain family ... XM_017004159.2 34.2% 35.4% (many diffs)
23 human 653781 POTEJ POTE ankyrin domain family ... NM_001277083.2 31.7% 32.5% (many diffs)
24 human 653781 POTEJ POTE ankyrin domain family ... XM_011511683.2 31.4% 32.2% (many diffs)
25 human 653781 POTEJ POTE ankyrin domain family ... XM_017004741.2 31.4% 32.2% (many diffs)
26 human 445582 POTEE POTE ankyrin domain family ... NM_001083538.2 30.8% 31.9% (many diffs)
27 human 445582 POTEE POTE ankyrin domain family ... XM_017004161.2 30.8% 31.9% (many diffs)
28 human 653269 POTEI POTE ankyrin domain family ... NM_001277406.2 30.7% 31.6% (many diffs)
29 human 653269 POTEI POTE ankyrin domain family ... XM_024453046.1 30.7% 31.6% (many diffs)
30 human 728378 POTEF POTE ankyrin domain family ... NM_001099771.2 30.6% 31.7% (many diffs)
31 human 653269 POTEI POTE ankyrin domain family ... XM_011511670.2 30.4% 31.3% (many diffs)
32 human 653269 POTEI POTE ankyrin domain family ... XM_017004732.2 30.4% 31.3% (many diffs)
33 human 728378 POTEF POTE ankyrin domain family ... XM_017004833.1 30.3% 31.4% (many diffs)
34 human 653269 POTEI POTE ankyrin domain family ... NM_001371926.1 30.1% 30.9% (many diffs)
35 mouse 11465 Actg1 actin, gamma, cytoplasmic 1 NM_009609.3 89.7% 100% (many diffs)
36 mouse 11461 Actb actin, beta NM_007393.5 89.3% 98.9% (many diffs)
37 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_001272041.1 85.3% 93.1% (many diffs)
38 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_009606.3 85.3% 93.1% (many diffs)
39 mouse 11464 Actc1 actin, alpha, cardiac muscle 1 NM_009608.4 81.9% 93.6% (many diffs)
40 mouse 11475 Acta2 actin, alpha 2, smooth musc... NM_007392.3 81.7% 93.6% (many diffs)
41 mouse 11468 Actg2 actin, gamma 2, smooth musc... NM_009610.2 81.6% 93.8% (many diffs)
42 mouse 11465 Actg1 actin, gamma, cytoplasmic 1 NM_001313923.1 60.2% 67.4% (many diffs)
43 mouse 11475 Acta2 actin, alpha 2, smooth musc... XM_006526606.2 59.5% 67.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1191
ORF length:
1125
Sequence:
1gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
61ttggcatgga agaagagatc gccgcgctgg tcattgacaa tggctccggc atgtgcaaag
121ctggttttgc tggggacgac gctccccgag ccgtgtttcc ttccatcgtc gggcgcccca
181gacaccaggg cgtcatggtg ggcatgggcc agaaggactc ctacgtgggc gacgaggccc
241agagcaagcg tggcatcctg accctgaagt accccattga gcatggcatc gtcaccaact
301gggacgacat ggagaagatc tggcaccaca ccttctacaa cgagctgcgc gtggccccgg
361aggagcaccc agtgctgctg accgaggccc ccctgaaccc caaggccaac agagagaaga
421tgactcagat tatgtttgag accttcaaca ccccggccat gtacgtggcc atccaggccg
481tgctgtccct ctacgcctct gggcgcacca ctggcattgt catggactct ggagacgggg
541tcacccacac ggtgcccatc tacgagggct acgccctccc ccacgccatc ctgcgtctgg
601acctggctgg ccgggacctg accgactacc tcatgaagat cctcactgag cgaggctaca
661gcttcaccac cacggccgag cgggaaatcg tgcgcgacat caaggagaag ctgtgctacg
721tcgccctgga cttcgagcag gagatggcca ccgccgcatc ctcctcttct ctggagaaga
781gctacgagct gcccgatggc caggtcatca ccattggcaa tgagcggttc cggtgtccgg
841aggcgctgtt ccagccttcc ttcctgggta tggaatcttg cggcatccac gagaccacct
901tcaactccat catgaagtgt gacgtggaca tccgcaaaga cctgtacgcc aacacggtgc
961tgtcgggcgg caccaccatg tatccgggca ttgccgacag gatgcagaag gagatcaccg
1021ccctggcgcc cagcaccatg aagatcaaga tcatcgcacc cccagagcgc aagtactcgg
1081tgtggatcgg tggctccatc ctggcctcac tgtccacctt ccagcagatg tggattagca
1141agcaggagta cgacgagtcg ggcccctcca tcgtccaccg caaatgcttc tnnccaactt
1201tcttgtacaa agttggcatt ataagaaagc attgcttatc aatttgttgc aacgaac

Download FASTA (ORF) (Full)