Construct: ORF ccsbBroad304_00015
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006671.1_s304c1, BRDN0000402330
- Derived from:
- ccsbBroadEn_00015
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTC1 (70)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_00015
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 70 | ACTC1 | actin alpha cardiac muscle 1 | NM_005159.5 | 100% | 100% | |
| 2 | human | 72 | ACTG2 | actin gamma 2, smooth muscle | NM_001615.4 | 85.9% | 98.6% | (many diffs) |
| 3 | human | 58 | ACTA1 | actin alpha 1, skeletal muscle | NM_001100.4 | 85.6% | 98.9% | (many diffs) |
| 4 | human | 59 | ACTA2 | actin alpha 2, smooth muscle | NM_001141945.2 | 85.6% | 98.4% | (many diffs) |
| 5 | human | 59 | ACTA2 | actin alpha 2, smooth muscle | NM_001320855.1 | 85.6% | 98.4% | (many diffs) |
| 6 | human | 59 | ACTA2 | actin alpha 2, smooth muscle | NM_001613.4 | 85.6% | 98.4% | (many diffs) |
| 7 | human | 60 | ACTB | actin beta | NM_001101.5 | 84.8% | 93.3% | (many diffs) |
| 8 | human | 71 | ACTG1 | actin gamma 1 | NM_001199954.2 | 83% | 93.6% | (many diffs) |
| 9 | human | 71 | ACTG1 | actin gamma 1 | NM_001614.5 | 83% | 93.6% | (many diffs) |
| 10 | human | 72 | ACTG2 | actin gamma 2, smooth muscle | NM_001199893.2 | 76.6% | 87.2% | (many diffs) |
| 11 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004734.2 | 47.4% | 49.4% | (many diffs) |
| 12 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004835.1 | 47.1% | 49.4% | (many diffs) |
| 13 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004743.2 | 47.1% | 49.1% | (many diffs) |
| 14 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004733.1 | 46.1% | 48.1% | (many diffs) |
| 15 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004834.1 | 45.8% | 48.1% | (many diffs) |
| 16 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004742.1 | 45.8% | 47.8% | (many diffs) |
| 17 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_011511216.2 | 32.7% | 34.2% | (many diffs) |
| 18 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_017004159.2 | 32.4% | 33.9% | (many diffs) |
| 19 | human | 653781 | POTEJ | POTE ankyrin domain family ... | NM_001277083.2 | 29.9% | 31.1% | (many diffs) |
| 20 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_011511683.2 | 29.6% | 30.8% | (many diffs) |
| 21 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004741.2 | 29.6% | 30.8% | (many diffs) |
| 22 | human | 445582 | POTEE | POTE ankyrin domain family ... | NM_001083538.2 | 29.1% | 30.5% | (many diffs) |
| 23 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_017004161.2 | 29.1% | 30.5% | (many diffs) |
| 24 | human | 653269 | POTEI | POTE ankyrin domain family ... | NM_001277406.2 | 29% | 30.2% | (many diffs) |
| 25 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_024453046.1 | 29% | 30.2% | (many diffs) |
| 26 | human | 728378 | POTEF | POTE ankyrin domain family ... | NM_001099771.2 | 28.8% | 30.2% | (many diffs) |
| 27 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_011511670.2 | 28.7% | 29.9% | (many diffs) |
| 28 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004732.2 | 28.7% | 29.9% | (many diffs) |
| 29 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004833.1 | 28.6% | 29.9% | (many diffs) |
| 30 | human | 653269 | POTEI | POTE ankyrin domain family ... | NM_001371926.1 | 28.4% | 29.6% | (many diffs) |
| 31 | mouse | 11464 | Actc1 | actin, alpha, cardiac muscle 1 | NM_009608.4 | 92.3% | 100% | (many diffs) |
| 32 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_001272041.1 | 87.7% | 98.9% | (many diffs) |
| 33 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_009606.3 | 87.7% | 98.9% | (many diffs) |
| 34 | mouse | 11468 | Actg2 | actin, gamma 2, smooth musc... | NM_009610.2 | 85.6% | 98.6% | (many diffs) |
| 35 | mouse | 11461 | Actb | actin, beta | NM_007393.5 | 84.8% | 93.3% | (many diffs) |
| 36 | mouse | 11475 | Acta2 | actin, alpha 2, smooth musc... | NM_007392.3 | 84% | 98.4% | (many diffs) |
| 37 | mouse | 11465 | Actg1 | actin, gamma, cytoplasmic 1 | NM_009609.3 | 83.1% | 93.6% | (many diffs) |
| 38 | mouse | 11475 | Acta2 | actin, alpha 2, smooth musc... | XM_006526606.2 | 62.9% | 71% | (many diffs) |
| 39 | mouse | 11465 | Actg1 | actin, gamma, cytoplasmic 1 | NM_001313923.1 | 56.5% | 63.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1197
- ORF length:
- 1131
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg tgacgacgag gagaccaccg ccctggtgtg cgacaacggc tctgggctgg 121 tgaaggccgg ctttgcgggc gatgacgcgc cccgcgctgt cttcccgtcc atcgtgggcc 181 gcccgcggca ccagggagtt atggtgggta tgggtcagaa ggactcctac gtaggtgatg 241 aagcccagag caagagaggc atcctgaccc tgaagtatcc catcgagcat ggtatcatca 301 ccaactggga cgacatggag aagatctggc accacacctt ctacaatgag ctccgtgtgg 361 ctcccgagga gcaccccacc ctgctcacag aggccccgct gaaccccaag gccaaccggg 421 agaagatgac tcagatcatg tttgagacct tcaatgtccc tgccatgtac gtggccatcc 481 aggcagtgct atccctgtat gcttctggcc gtaccacagg cattgttctg gactctgggg 541 atggtgtaac tcacaatgtc cccatctatg agggctacgc tttgccccat gccatcatgc 601 gtctggatct ggctggtcgg gacctcactg actacctcat gaagatcctc actgagcgtg 661 gctactcctt tgtcaccact gctgaacgtg aaattgtccg tgacattaaa gagaagctgt 721 gctatgtcgc cctggatttt gagaatgaga tggccacagc tgcctcttcc tcctccctgg 781 agaagagcta tgaactgcct gatggccaag tcatcactat tggcaatgag cgcttccgct 841 gtcctgagac actcttccag ccctccttca ttggtatgga atctgctggc atccatgaaa 901 caacttacaa tagcatcatg aagtgtgaca ttgatatccg caaggacctg tatgccaaca 961 atgtcttatc tggaggcacc actatgtacc ctggtattgc tgatcgtatg cagaaggaaa 1021 tcactgctct ggctcctagc accatgaaga ttaagattat tgctccccct gagcgtaaat 1081 actctgtctg gattgggggc tccatcctgg cctctctgtc caccttccag caaatgtgga 1141 ttagcaagca agagtacgat gaggcaggcc catccattgt ccaccgcaaa tgcttctacc 1201 caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt 1261 ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga 1321 tta