Construct: ORF TRCN0000471742
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017937.1_s317c1
- Derived from:
- ccsbBroadEn_13808
- DNA Barcode:
- AATGGGAGTTCTCACGTCAGGTCC
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTG1 (71)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000471742
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 71 | ACTG1 | actin gamma 1 | NM_001199954.2 | 99.9% | 99.4% | 1121delG |
| 2 | human | 71 | ACTG1 | actin gamma 1 | NM_001614.5 | 99.9% | 99.4% | 1121delG |
| 3 | human | 60 | ACTB | actin beta | NM_001101.5 | 91.2% | 98.4% | (many diffs) |
| 4 | human | 58 | ACTA1 | actin alpha 1, skeletal muscle | NM_001100.4 | 85.7% | 92.5% | (many diffs) |
| 5 | human | 70 | ACTC1 | actin alpha cardiac muscle 1 | NM_005159.5 | 82.9% | 93.1% | (many diffs) |
| 6 | human | 72 | ACTG2 | actin gamma 2, smooth muscle | NM_001615.4 | 82.3% | 93.3% | (many diffs) |
| 7 | human | 59 | ACTA2 | actin alpha 2, smooth muscle | NM_001141945.2 | 82.2% | 93.1% | (many diffs) |
| 8 | human | 59 | ACTA2 | actin alpha 2, smooth muscle | NM_001320855.1 | 82.2% | 93.1% | (many diffs) |
| 9 | human | 59 | ACTA2 | actin alpha 2, smooth muscle | NM_001613.4 | 82.2% | 93.1% | (many diffs) |
| 10 | human | 345651 | ACTBL2 | actin beta like 2 | NM_001017992.4 | 80.7% | 90.4% | (many diffs) |
| 11 | human | 72 | ACTG2 | actin gamma 2, smooth muscle | NM_001199893.2 | 72.8% | 82.1% | (many diffs) |
| 12 | human | 71 | ACTG1 | actin gamma 1 | NR_037688.3 | 63.5% | 1_72del;1193delG;1198_1770del | |
| 13 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004734.2 | 50.3% | 51.4% | (many diffs) |
| 14 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004743.2 | 50.2% | 51.1% | (many diffs) |
| 15 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004835.1 | 50.1% | 51.7% | (many diffs) |
| 16 | human | 648740 | ACTG1P4 | actin gamma 1 pseudogene 4 | NR_024438.2 | 49.1% | (many diffs) | |
| 17 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004733.1 | 49% | 50% | (many diffs) |
| 18 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004742.1 | 48.8% | 49.7% | (many diffs) |
| 19 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004834.1 | 48.8% | 50.3% | (many diffs) |
| 20 | human | 644936 | LOC644936 | actin beta pseudogene | NR_004845.2 | 39.9% | (many diffs) | |
| 21 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_011511216.2 | 34.6% | 35.6% | (many diffs) |
| 22 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_017004159.2 | 34.2% | 35.2% | (many diffs) |
| 23 | human | 653781 | POTEJ | POTE ankyrin domain family ... | NM_001277083.2 | 31.7% | 32.3% | (many diffs) |
| 24 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_011511683.2 | 31.4% | 32% | (many diffs) |
| 25 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004741.2 | 31.4% | 32% | (many diffs) |
| 26 | human | 445582 | POTEE | POTE ankyrin domain family ... | NM_001083538.2 | 30.8% | 31.7% | (many diffs) |
| 27 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_017004161.2 | 30.8% | 31.7% | (many diffs) |
| 28 | human | 653269 | POTEI | POTE ankyrin domain family ... | NM_001277406.2 | 30.7% | 31.4% | (many diffs) |
| 29 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_024453046.1 | 30.7% | 31.4% | (many diffs) |
| 30 | human | 728378 | POTEF | POTE ankyrin domain family ... | NM_001099771.2 | 30.6% | 31.6% | (many diffs) |
| 31 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_011511670.2 | 30.4% | 31.1% | (many diffs) |
| 32 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004732.2 | 30.4% | 31.1% | (many diffs) |
| 33 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004833.1 | 30.3% | 31.3% | (many diffs) |
| 34 | human | 653269 | POTEI | POTE ankyrin domain family ... | NM_001371926.1 | 30.1% | 30.8% | (many diffs) |
| 35 | mouse | 11465 | Actg1 | actin, gamma, cytoplasmic 1 | NM_009609.3 | 89.7% | 99.4% | (many diffs) |
| 36 | mouse | 11461 | Actb | actin, beta | NM_007393.5 | 89.3% | 98.4% | (many diffs) |
| 37 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_001272041.1 | 85.3% | 92.5% | (many diffs) |
| 38 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_009606.3 | 85.3% | 92.5% | (many diffs) |
| 39 | mouse | 11464 | Actc1 | actin, alpha, cardiac muscle 1 | NM_009608.4 | 81.9% | 93.1% | (many diffs) |
| 40 | mouse | 11475 | Acta2 | actin, alpha 2, smooth musc... | NM_007392.3 | 81.7% | 93.1% | (many diffs) |
| 41 | mouse | 11468 | Actg2 | actin, gamma 2, smooth musc... | NM_009610.2 | 81.6% | 93.3% | (many diffs) |
| 42 | mouse | 11465 | Actg1 | actin, gamma, cytoplasmic 1 | NM_001313923.1 | 60.2% | 66.9% | (many diffs) |
| 43 | mouse | 11475 | Acta2 | actin, alpha 2, smooth musc... | XM_006526606.2 | 59.5% | 67.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1191
- ORF length:
- 1125
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga agaagagatc gccgcgctgg tcattgacaa tggctccggc atgtgcaaag 121 ctggttttgc tggggacgac gctccccgag ccgtgtttcc ttccatcgtc gggcgcccca 181 gacaccaggg cgtcatggtg ggcatgggcc agaaggactc ctacgtgggc gacgaggccc 241 agagcaagcg tggcatcctg accctgaagt accccattga gcatggcatc gtcaccaact 301 gggacgacat ggagaagatc tggcaccaca ccttctacaa cgagctgcgc gtggccccgg 361 aggagcaccc agtgctgctg accgaggccc ccctgaaccc caaggccaac agagagaaga 421 tgactcagat tatgtttgag accttcaaca ccccggccat gtacgtggcc atccaggccg 481 tgctgtccct ctacgcctct gggcgcacca ctggcattgt catggactct ggagacgggg 541 tcacccacac ggtgcccatc tacgagggct acgccctccc ccacgccatc ctgcgtctgg 601 acctggctgg ccgggacctg accgactacc tcatgaagat cctcactgag cgaggctaca 661 gcttcaccac cacggccgag cgggaaaTCG TGCGCGACAT CAAGGAGAAG CTGTGCTACG 721 TCGCCCTGGA CTTCGAGCAG GAGATGGCCA CCGCCGCATC CTCCTCTTCT CTGGAGAAGA 781 GCTACGAGCT GCCCGATGGC CAGGTCATCA CCATTGGCAA TGAGCGGTTC CGGTGTCCGG 841 AGGCGCTGTT CCAGCCTTCC TTCCTGGGTA TGGAATCTTG CGGCATCCAC GAGACCACCT 901 TCAACTCCAT CATGAAGTGT GACGTGGACA TCCGCAAAGA CCTGTACGCC AACACGGTGC 961 TGTCGGGCGG CACCACCATG TACCCGGGCA TTGCCGACAG GATGCAGAAG GAGATCACCG 1021 CCCTGGCGCC CAGCACCATG AAGATCAAGA TCATCGCACC CCCAGAGCGC AAGTACTCGG 1081 TGTGGATCGG TGGCTCCATC CTGGCCTCAC TGTCCACCTT CCAGCAGATG TGGATTAGCA 1141 AGCAGGAGTA CGACGAGTCG GGCCCCTCCA TCGTCCACCG CAAATCTTCT ACCCAACTTT 1201 CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC 1261 TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC 1321 TTGTGGAAAG GACGAAATGG GAGTTCTCAC GTCAGGTCCA CGCGTTAAGT Cgacaatcaa 1381 cctctggatt acaaaatttg tgaaagatt