Construct: ORF ccsbBroad304_05763

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF011314.1_s304c1, BRDN0000410619
Derived from:
ccsbBroadEn_05763
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
ACTB (60)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_05763
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 60 ACTB actin beta NM_001101.5 99.9% 99.7% 289G>C
2 human 71 ACTG1 actin gamma 1 NM_001199954.2 91.2% 98.6% (many diffs)
3 human 71 ACTG1 actin gamma 1 NM_001614.5 91.2% 98.6% (many diffs)
4 human 58 ACTA1 actin alpha 1, skeletal muscle NM_001100.4 85.7% 92.5% (many diffs)
5 human 70 ACTC1 actin alpha cardiac muscle 1 NM_005159.5 84.7% 93.1% (many diffs)
6 human 72 ACTG2 actin gamma 2, smooth muscle NM_001615.4 84.1% 92.8% (many diffs)
7 human 59 ACTA2 actin alpha 2, smooth muscle NM_001141945.2 82% 93.1% (many diffs)
8 human 59 ACTA2 actin alpha 2, smooth muscle NM_001320855.1 82% 93.1% (many diffs)
9 human 59 ACTA2 actin alpha 2, smooth muscle NM_001613.4 82% 93.1% (many diffs)
10 human 345651 ACTBL2 actin beta like 2 NM_001017992.4 81.5% 90.9% (many diffs)
11 human 72 ACTG2 actin gamma 2, smooth muscle NM_001199893.2 74.5% 81.6% (many diffs)
12 human 71 ACTG1 actin gamma 1 NR_037688.3 57.9% (many diffs)
13 human 728378 POTEF POTE ankyrin domain family ... XM_017004835.1 52.6% 52% (many diffs)
14 human 653269 POTEI POTE ankyrin domain family ... XM_017004734.2 52.6% 51.9% (many diffs)
15 human 653781 POTEJ POTE ankyrin domain family ... XM_017004743.2 52.5% 51.5% (many diffs)
16 human 728378 POTEF POTE ankyrin domain family ... XM_017004834.1 51.2% 50.6% (many diffs)
17 human 653269 POTEI POTE ankyrin domain family ... XM_017004733.1 51.2% 50.5% (many diffs)
18 human 653781 POTEJ POTE ankyrin domain family ... XM_017004742.1 51.1% 50.2% (many diffs)
19 human 644936 LOC644936 actin beta pseudogene NR_004845.2 41.3% (many diffs)
20 human 445582 POTEE POTE ankyrin domain family ... XM_011511216.2 36.2% 35.9% (many diffs)
21 human 445582 POTEE POTE ankyrin domain family ... XM_017004159.2 35.9% 35.5% (many diffs)
22 human 653781 POTEJ POTE ankyrin domain family ... NM_001277083.2 33.2% 32.6% (many diffs)
23 human 653781 POTEJ POTE ankyrin domain family ... XM_011511683.2 32.9% 32.3% (many diffs)
24 human 653781 POTEJ POTE ankyrin domain family ... XM_017004741.2 32.9% 32.3% (many diffs)
25 human 445582 POTEE POTE ankyrin domain family ... NM_001083538.2 32.3% 32% (many diffs)
26 human 445582 POTEE POTE ankyrin domain family ... XM_017004161.2 32.3% 32% (many diffs)
27 human 728378 POTEF POTE ankyrin domain family ... NM_001099771.2 32.1% 31.8% (many diffs)
28 human 653269 POTEI POTE ankyrin domain family ... NM_001277406.2 32.1% 31.7% (many diffs)
29 human 653269 POTEI POTE ankyrin domain family ... XM_024453046.1 32.1% 31.7% (many diffs)
30 human 728378 POTEF POTE ankyrin domain family ... XM_017004833.1 31.8% 31.5% (many diffs)
31 human 653269 POTEI POTE ankyrin domain family ... XM_011511670.2 31.8% 31.4% (many diffs)
32 human 653269 POTEI POTE ankyrin domain family ... XM_017004732.2 31.8% 31.4% (many diffs)
33 human 653269 POTEI POTE ankyrin domain family ... NM_001371926.1 31.5% 31% (many diffs)
34 mouse 11461 Actb actin, beta NM_007393.5 91.8% 99.7% (many diffs)
35 mouse 11465 Actg1 actin, gamma, cytoplasmic 1 NM_009609.3 89.1% 98.6% (many diffs)
36 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_001272041.1 84.3% 92.5% (many diffs)
37 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_009606.3 84.3% 92.5% (many diffs)
38 mouse 11468 Actg2 actin, gamma 2, smooth musc... NM_009610.2 83% 92.8% (many diffs)
39 mouse 11464 Actc1 actin, alpha, cardiac muscle 1 NM_009608.4 82.7% 93.1% (many diffs)
40 mouse 11475 Acta2 actin, alpha 2, smooth musc... NM_007392.3 82.3% 93.1% (many diffs)
41 mouse 11465 Actg1 actin, gamma, cytoplasmic 1 NM_001313923.1 60.3% 67.4% (many diffs)
42 mouse 11475 Acta2 actin, alpha 2, smooth musc... XM_006526606.2 60% 67.1% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1191
ORF length:
1125
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga tgatgatatc gccgcgctcg tcgtcgacaa cggctccggc atgtgcaagg
121ccggcttcgc gggcgacgat gccccccggg ccgtcttccc ctccatcgtg gggcgcccca
181ggcaccaggg cgtgatggtg ggcatgggtc agaaggattc ctatgtgggc gacgaggccc
241agagcaagag aggcatcctc accctgaagt accccatcga gcacggcatc gtcaccaact
301gggacgacat ggagaaaatc tggcaccaca ccttctacaa tgagctgcgt gtgcctcccg
361aggagcaccc cgtgctgctg accgaggccc ccctgaaccc caaggccaac cgcgagaaga
421tgacccagat catgtttgag accttcaaca ccccagccat gtacgttgct atccaggctg
481tgctatccct gtacgcctct ggccgtacca ctggcatcgt gatggactcc ggtgacgggg
541tcacccacac tgtgcccatc tacgaggggt atgccctccc ccatgccatc ctGCGTCTGG
601ACCTGGCTGG CCGGGACCTG ACTGACTACC TCATGAAGAT CCTCACCGAG CGCGGCTACA
661GCTTCACCAC CACGGCCGAG CGGGAAATCG TGCGTGACAT TAAGGAGAAG CTGTGCTACG
721TCGCCCTGGA CTTCGAGCAA GAGATGGCCA CGGCTGCTTC CAGCTCCTCC CTGGAGAAGA
781GCTACGAGCT GCCTGACGGC CAGGTCATCA CCATTGGCAA TGAGCGGTTC CGCTGCCCTG
841AGGCACTCTT CCAGCCTTCC TTCCTGGGCA TGGAGTCCTG TGGCATCCAC GAAACTACCT
901TCAACTCCAT CATGAAGTGT GACGTGGACA TCCGCAAAGA CCTGTACGCC AACACAGTGC
961TGTCTGGCGG CACCACCATG TACCCTGGCA TTGCCGACAG GATGCAGAAG GAGATCACTG
1021CCCTGGCACC CAGCACAATG AAGATCAAGA TCATTGCTCC TCCTGAGCGC AAGTACTCCG
1081TGTGGATCGG CGGCTCCATC CTGGCCTCGC TGTCCACCTT CCAGCAGATG TGGATCAGCA
1141AGCAGGAGTA TGACGAGTCC GGCCCCTCCA TCGTCCACCG CAAATGCTTC TACCCAACTT
1201TCTTGTACaa agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt
1261agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)