Construct: ORF TRCN0000470279
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011314.1_s317c1
- Derived from:
- ccsbBroadEn_05763
- DNA Barcode:
- TCAAGCCTGGAACGGGCTCACGTC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ACTB (60)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470279
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 60 | ACTB | actin beta | NM_001101.5 | 99.9% | 99.7% | 289G>C |
| 2 | human | 71 | ACTG1 | actin gamma 1 | NM_001199954.2 | 91.2% | 98.6% | (many diffs) |
| 3 | human | 71 | ACTG1 | actin gamma 1 | NM_001614.5 | 91.2% | 98.6% | (many diffs) |
| 4 | human | 58 | ACTA1 | actin alpha 1, skeletal muscle | NM_001100.4 | 85.7% | 92.5% | (many diffs) |
| 5 | human | 70 | ACTC1 | actin alpha cardiac muscle 1 | NM_005159.5 | 84.7% | 93.1% | (many diffs) |
| 6 | human | 72 | ACTG2 | actin gamma 2, smooth muscle | NM_001615.4 | 84.1% | 92.8% | (many diffs) |
| 7 | human | 59 | ACTA2 | actin alpha 2, smooth muscle | NM_001141945.2 | 82% | 93.1% | (many diffs) |
| 8 | human | 59 | ACTA2 | actin alpha 2, smooth muscle | NM_001320855.1 | 82% | 93.1% | (many diffs) |
| 9 | human | 59 | ACTA2 | actin alpha 2, smooth muscle | NM_001613.4 | 82% | 93.1% | (many diffs) |
| 10 | human | 345651 | ACTBL2 | actin beta like 2 | NM_001017992.4 | 81.5% | 90.9% | (many diffs) |
| 11 | human | 72 | ACTG2 | actin gamma 2, smooth muscle | NM_001199893.2 | 74.5% | 81.6% | (many diffs) |
| 12 | human | 71 | ACTG1 | actin gamma 1 | NR_037688.3 | 57.9% | (many diffs) | |
| 13 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004835.1 | 52.6% | 52% | (many diffs) |
| 14 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004734.2 | 52.6% | 51.9% | (many diffs) |
| 15 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004743.2 | 52.5% | 51.5% | (many diffs) |
| 16 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004834.1 | 51.2% | 50.6% | (many diffs) |
| 17 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004733.1 | 51.2% | 50.5% | (many diffs) |
| 18 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004742.1 | 51.1% | 50.2% | (many diffs) |
| 19 | human | 644936 | LOC644936 | actin beta pseudogene | NR_004845.2 | 41.3% | (many diffs) | |
| 20 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_011511216.2 | 36.2% | 35.9% | (many diffs) |
| 21 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_017004159.2 | 35.9% | 35.5% | (many diffs) |
| 22 | human | 653781 | POTEJ | POTE ankyrin domain family ... | NM_001277083.2 | 33.2% | 32.6% | (many diffs) |
| 23 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_011511683.2 | 32.9% | 32.3% | (many diffs) |
| 24 | human | 653781 | POTEJ | POTE ankyrin domain family ... | XM_017004741.2 | 32.9% | 32.3% | (many diffs) |
| 25 | human | 445582 | POTEE | POTE ankyrin domain family ... | NM_001083538.2 | 32.3% | 32% | (many diffs) |
| 26 | human | 445582 | POTEE | POTE ankyrin domain family ... | XM_017004161.2 | 32.3% | 32% | (many diffs) |
| 27 | human | 728378 | POTEF | POTE ankyrin domain family ... | NM_001099771.2 | 32.1% | 31.8% | (many diffs) |
| 28 | human | 653269 | POTEI | POTE ankyrin domain family ... | NM_001277406.2 | 32.1% | 31.7% | (many diffs) |
| 29 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_024453046.1 | 32.1% | 31.7% | (many diffs) |
| 30 | human | 728378 | POTEF | POTE ankyrin domain family ... | XM_017004833.1 | 31.8% | 31.5% | (many diffs) |
| 31 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_011511670.2 | 31.8% | 31.4% | (many diffs) |
| 32 | human | 653269 | POTEI | POTE ankyrin domain family ... | XM_017004732.2 | 31.8% | 31.4% | (many diffs) |
| 33 | human | 653269 | POTEI | POTE ankyrin domain family ... | NM_001371926.1 | 31.5% | 31% | (many diffs) |
| 34 | mouse | 11461 | Actb | actin, beta | NM_007393.5 | 91.8% | 99.7% | (many diffs) |
| 35 | mouse | 11465 | Actg1 | actin, gamma, cytoplasmic 1 | NM_009609.3 | 89.1% | 98.6% | (many diffs) |
| 36 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_001272041.1 | 84.3% | 92.5% | (many diffs) |
| 37 | mouse | 11459 | Acta1 | actin, alpha 1, skeletal mu... | NM_009606.3 | 84.3% | 92.5% | (many diffs) |
| 38 | mouse | 11468 | Actg2 | actin, gamma 2, smooth musc... | NM_009610.2 | 83% | 92.8% | (many diffs) |
| 39 | mouse | 11464 | Actc1 | actin, alpha, cardiac muscle 1 | NM_009608.4 | 82.7% | 93.1% | (many diffs) |
| 40 | mouse | 11475 | Acta2 | actin, alpha 2, smooth musc... | NM_007392.3 | 82.3% | 93.1% | (many diffs) |
| 41 | mouse | 11465 | Actg1 | actin, gamma, cytoplasmic 1 | NM_001313923.1 | 60.3% | 67.4% | (many diffs) |
| 42 | mouse | 11475 | Acta2 | actin, alpha 2, smooth musc... | XM_006526606.2 | 60% | 67.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1191
- ORF length:
- 1125
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tgatgatatc gccgcgctcg tcgtcgacaa cggctccggc atgtgcaagg 121 ccggcttcgc gggcgacgat gccccccggg ccgtcttccc ctccatcgtg gggcgcccca 181 ggcaccaggg cgtgatggtg ggcatgggtc agaaggattc ctatgtgggc gacgaggccc 241 agagcaagag aggcatcctc accctgaagt accccatcga gcacggcatc gtcaccaact 301 gggacgacat ggagaaaatc tggcaccaca ccttctacaa tgagctgcgt gtgcctcccg 361 aggagcaccc cgtgctgctg accgaggccc ccctgaaccc caaggccaac cgcgagaaga 421 tgacccagat catgtttgag accttcaaca ccccagccat gtacgttgct atccaggctg 481 tgctatccct gtacgcctct ggccgtacca ctggcatcgt gatggactcc ggtgacgggg 541 tcacccacac tgtgcccatc tacgaggggt atgccctccc ccatgccatc ctgcgtctgg 601 acctggctgg ccgggacctg actgactacc tcatgaagat cctcaccgag cgcggctaca 661 gcttcaccac cacggccgag cgggaaatcg tgcgtgacat taaggagaag ctgtgctacg 721 tcgccctgga cttcgagcaa gagatggcca cggctgcttc cagctcctcc ctggagaaga 781 gctacgagct gcctgacggc caggtcatca ccattggcaa tgagcggttc cgctgcccTG 841 AGGCACTCTT CCAGCCTTCC TTCCTGGGCA TGGAGTCCTG TGGCATCCAC GAAACTACCT 901 TCAACTCCAT CATGAAGTGT GACGTGGACA TCCGCAAAGA CCTGTACGCC AACACAGTGC 961 TGTCTGGCGG CACCACCATG TACCCTGGCA TTGCCGACAG GATGCAGAAG GAGATCACTG 1021 CCCTGGCACC CAGCACAATG AAGATCAAGA TCATTGCTCC TCCTGAGCGC AAGTACTCCG 1081 TGTGGATCGG CGGCTCCATC CTGGCCTCGC TGTCCACCTT CCAGCAGATG TGGATCAGCA 1141 AGCAGGAGTA TGACGAGTCC GGCCCCTCCA TCGTCCACCG CAAATGCTTC TACCCAACTT 1201 TCTTGTACAA AGTGGTTGAT ATCGGTAAGC CTATCCCTAA CCCTCTCCTC GGTCTCGATT 1261 CTACGTAGTA ATGAACTAGT CCGTAACTTG AAAGTATTTC GATTTCTTGG CTTTATATAT 1321 CTTGTGGAAA GGACGATCAA GCCTGGAACG GGCTCACGTC ACGCGTTAAG TCgacaatca 1381 acctctggat tacaaaattt gtgaaagatt