Construct: ORF ccsbBroad304_00014

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF010688.1_s304c1, BRDN0000402331
Derived from:
ccsbBroadEn_00014
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Has stop codon in insert

Originally Annotated References:

Gene:
ACTA2 (59)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_00014
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 59 ACTA2 actin alpha 2, smooth muscle NM_001141945.2 100% 100%
2 human 59 ACTA2 actin alpha 2, smooth muscle NM_001320855.1 100% 100%
3 human 59 ACTA2 actin alpha 2, smooth muscle NM_001613.4 100% 100%
4 human 70 ACTC1 actin alpha cardiac muscle 1 NM_005159.5 85.6% 98.4% (many diffs)
5 human 58 ACTA1 actin alpha 1, skeletal muscle NM_001100.4 84.7% 97.8% (many diffs)
6 human 72 ACTG2 actin gamma 2, smooth muscle NM_001615.4 84.7% 99.2% (many diffs)
7 human 71 ACTG1 actin gamma 1 NM_001199954.2 82.3% 93.6% (many diffs)
8 human 71 ACTG1 actin gamma 1 NM_001614.5 82.3% 93.6% (many diffs)
9 human 60 ACTB actin beta NM_001101.5 81.9% 93.3% (many diffs)
10 human 72 ACTG2 actin gamma 2, smooth muscle NM_001199893.2 75.2% 87.7% (many diffs)
11 human 653269 POTEI POTE ankyrin domain family ... XM_017004734.2 46.9% 49.3% (many diffs)
12 human 728378 POTEF POTE ankyrin domain family ... XM_017004835.1 46.8% 49.3% (many diffs)
13 human 653781 POTEJ POTE ankyrin domain family ... XM_017004743.2 46.8% 49% (many diffs)
14 human 653269 POTEI POTE ankyrin domain family ... XM_017004733.1 45.6% 48% (many diffs)
15 human 728378 POTEF POTE ankyrin domain family ... XM_017004834.1 45.6% 48% (many diffs)
16 human 653781 POTEJ POTE ankyrin domain family ... XM_017004742.1 45.5% 47.7% (many diffs)
17 human 445582 POTEE POTE ankyrin domain family ... XM_011511216.2 32.2% 34.1% (many diffs)
18 human 445582 POTEE POTE ankyrin domain family ... XM_017004159.2 31.9% 33.8% (many diffs)
19 human 653781 POTEJ POTE ankyrin domain family ... NM_001277083.2 29.6% 31% (many diffs)
20 human 653781 POTEJ POTE ankyrin domain family ... XM_011511683.2 29.3% 30.7% (many diffs)
21 human 653781 POTEJ POTE ankyrin domain family ... XM_017004741.2 29.3% 30.7% (many diffs)
22 human 445582 POTEE POTE ankyrin domain family ... NM_001083538.2 28.7% 30.4% (many diffs)
23 human 445582 POTEE POTE ankyrin domain family ... XM_017004161.2 28.7% 30.4% (many diffs)
24 human 653269 POTEI POTE ankyrin domain family ... NM_001277406.2 28.6% 30.1% (many diffs)
25 human 653269 POTEI POTE ankyrin domain family ... XM_024453046.1 28.6% 30.1% (many diffs)
26 human 728378 POTEF POTE ankyrin domain family ... NM_001099771.2 28.6% 30.1% (many diffs)
27 human 653269 POTEI POTE ankyrin domain family ... XM_011511670.2 28.4% 29.8% (many diffs)
28 human 653269 POTEI POTE ankyrin domain family ... XM_017004732.2 28.4% 29.8% (many diffs)
29 human 728378 POTEF POTE ankyrin domain family ... XM_017004833.1 28.3% 29.8% (many diffs)
30 human 653269 POTEI POTE ankyrin domain family ... NM_001371926.1 28% 29.5% (many diffs)
31 mouse 11475 Acta2 actin, alpha 2, smooth musc... NM_007392.3 91.4% 100% (many diffs)
32 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_001272041.1 86.2% 97.8% (many diffs)
33 mouse 11459 Acta1 actin, alpha 1, skeletal mu... NM_009606.3 86.2% 97.8% (many diffs)
34 mouse 11468 Actg2 actin, gamma 2, smooth musc... NM_009610.2 84.7% 99.2% (many diffs)
35 mouse 11464 Actc1 actin, alpha, cardiac muscle 1 NM_009608.4 84.3% 98.4% (many diffs)
36 mouse 11461 Actb actin, beta NM_007393.5 82.4% 93.3% (many diffs)
37 mouse 11465 Actg1 actin, gamma, cytoplasmic 1 NM_009609.3 81.4% 93.6% (many diffs)
38 mouse 11475 Acta2 actin, alpha 2, smooth musc... XM_006526606.2 67.1% 72.6% (many diffs)
39 mouse 11465 Actg1 actin, gamma, cytoplasmic 1 NM_001313923.1 55.5% 63.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1197
ORF length:
1131
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtg tgaagaagag gacagcactg ccttggtgtg tgacaatggc tctgggctct
121gtaaggccgg ctttgctggg gacgatgctc ccagggctgt tttcccatcc attgtgggac
181gtcccagaca tcagggggtg atggtgggaa tgggacaaaa agacagctac gtgggtgacg
241aagcacagag caaaagagga atcctgaccc tgaagtaccc gatagaacat ggcatcatca
301ccaactggga cgacatggaa aagatctggc accactcttt ctacaatgag cttcgtgttg
361cccctgaaga gcatcccacc ctgctcacgg aggcacccct gaaccccaag gccaaccggg
421agaaaatgac tcaaattatg tttgagactt tcaatgtccc agccatgtat gtggctatcc
481aggcggtgct gtctctctat gcctctggac gcacaactgg catcgtgctg gactctggag
541atggtgtcac ccacaatgtc cccatctatg agggctatgc cttgccccat gccatcatgc
601gtctggatct ggctggccga gatctcactg actacctcat gaagatcctg actgagcgtg
661gctattcctt cgttactact gctgagcgtg agattgtccg ggacatcaag gagaaactgt
721gttatgtagc tctggacttt gaaaatgaga tggccactgc cgcatcctca tcctcccttg
781agaagagtta cgagttgcct gatgggcaag tgatcaccat cggaaatgaa cgtttccgct
841gcccagagac cctgttccag ccatccttca tcgggatgga gtctgctggc atccatgaaa
901ccacctacaa cagcatcatg aagtgtgata ttgacatcag gaaggacctc tatgctaaca
961atgtcctatc agggggcacc actatgtacc ctggcattgc cgaccgaatg cagaaggaga
1021tcacggccct agcacccagc accatgaaga tcaagatcat tgcccctccg gagcgcaaat
1081actctgtctg gatcggtggc tccatcctgg cctctctgtc caccttccag cagatgtgga
1141tcagcaaaca ggaatacgat gaagccgggc cttccattgt ccaccgcaaa tgcttctaac
1201caactttctt gtacaaagtg gttggtaagc ctatccctaa ccctctcctc ggtctcgatt
1261ctacgtagta atgagctagc gctaaccggt ggcgcgttaa gtcgacaatc aacctctgga
1321tta

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