Transcript: Human XM_017026991.1

PREDICTED: Homo sapiens mitogen-activated protein kinase kinase 2 (MAP2K2), transcript variant X4, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MAP2K2 (5605)
Length:
1364
CDS:
310..1068

Additional Resources:

NCBI RefSeq record:
XM_017026991.1
NBCI Gene record:
MAP2K2 (5605)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017026991.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199414 CGAATGCAACTCGCCGTACAT pLKO.1 678 CDS 100% 4.950 3.465 N MAP2K2 n/a
2 TRCN0000342310 CGAATGCAACTCGCCGTACAT pLKO_005 678 CDS 100% 4.950 3.465 N MAP2K2 n/a
3 TRCN0000196991 GCATTTGCATGGAACACATGG pLKO.1 740 CDS 100% 4.050 2.835 N MAP2K2 n/a
4 TRCN0000007005 CCAACATCCTCGTGAACTCTA pLKO.1 902 CDS 100% 4.950 2.475 Y MAP2K2 n/a
5 TRCN0000195427 CGAACTCAAAGACGATGACTT pLKO.1 504 CDS 100% 4.950 2.475 Y MAP2K2 n/a
6 TRCN0000342309 CGAACTCAAAGACGATGACTT pLKO_005 504 CDS 100% 4.950 2.475 Y MAP2K2 n/a
7 TRCN0000011062 CAACATCCTCGTGAACTCTAG pLKO.1 903 CDS 100% 4.050 2.025 Y MAP2K2 n/a
8 TRCN0000342311 CAACATCCTCGTGAACTCTAG pLKO_005 903 CDS 100% 4.050 2.025 Y MAP2K2 n/a
9 TRCN0000055065 CCTCCGAGAGAAGCACCAGAT pLKO.1 858 CDS 100% 1.350 0.675 Y Map2k2 n/a
10 TRCN0000288078 CCTCCGAGAGAAGCACCAGAT pLKO_005 858 CDS 100% 1.350 0.675 Y Map2k2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017026991.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 TRCN0000487698 GCGCTTTTCCTGCACCTTGACTTA pLX_317 23.3% 78.8% 74.2% V5 (not translated due to prior stop codon) (many diffs) n/a
2 ccsbBroadEn_10634 pDONR223 100% 62.3% 60.3% None (many diffs) n/a
3 ccsbBroad304_10634 pLX_304 0% 62.3% 60.3% V5 (many diffs) n/a
4 TRCN0000477744 AAGCGGTCACCAGAATGAGGGTTC pLX_317 27.6% 62.3% 60.3% V5 (many diffs) n/a
5 TRCN0000488054 AAGGTTTGCCGATAAATCTTAGCG pLX_317 23.1% 61.6% 60.2% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000487985 AGCCCTCCCGCTAGCATAACCTAG pLX_317 26.4% 61.6% 60% V5 (many diffs) n/a
7 ccsbBroadEn_14808 pDONR223 0% 61.5% 60.2% None (many diffs) n/a
8 ccsbBroad304_14808 pLX_304 0% 61.5% 60.2% V5 (many diffs) n/a
9 TRCN0000465915 AACCGTCGGGCCCACCATGAAATT pLX_317 17.9% 61.5% 60.2% V5 (many diffs) n/a
10 TRCN0000465689 TGACCGAAAAAAAATTTTTCGTTT pLX_317 17.9% 61.3% 77.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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